Enhanced innate type 2 immune response in peripheral blood from patients with asthma
Open accessAbstract
Background In mice, group 2 innate lymphoid cells (ILC2s) likely mediate helminth immunity, inflammation, and tissue repair and remodeling. However, the involvement of ILC2s in human diseases, such as asthma, is not well understood.Objectives The goals of this study were to investigate whether peripheral blood specimens can be used to monitor innate type 2 immunity in human subjects and to examine whether ILC2s are involved in human asthma.Methods PBMCs from subjects with allergic asthma (AA), subjects with allergic rhinitis (AR), or healthy control (HC) subjects were cultured in vitro with IL-25 or IL-33. Flow cytometry and cell sorting were used to identify, isolate, and quantitate ILC2s in PBMCs.Results Human PBMCs produced IL-5 and IL-13 when stimulated with IL-33 or IL-25 in the presence of IL-2 without antigens. In addition, IL-7 or thymic stromal lymphopoietin were able to replace IL-2. The cell population with phenotypic ILC2 characteristics, lineage(-)CD127(+)CRTH2(+) cells, responded to IL-33 and produced large quantities of IL-5 and IL-13 but undetectable levels of IL-4. PBMCs from subjects with AA produced significantly larger amounts of IL-5 and IL-13 in response to IL-25 or IL-33 than from subjects with AR or HC. The prevalence of ILC2s in blood was greater in the AA group than in the AR group or the HC group.Conclusions Innate type 2 immune responses are increased in asthma but not in AR, suggesting potential differences in the immunopathogenesis of these diseases. Peripheral blood is useful for evaluating innate type 2 immunity in humans.
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Innate lymphoid cells (ILCs) have been discovered as potential key players in pathogenesis of Th2-type diseases such as AR, CRSwNP, and asthma.
“An interesting recent development was the discovery of innate lymphoid cells (ILCs) as potential key players in the pathogenesis of Th2-type diseases such as AR, CRSwNP, and asthma.328-330”
From International Consensus Statement on Allergy and Rhinology: Allergic Rhinitis (2018) · cites this paper as [330]
The Introduction reports increased ILC2s in allergic asthma and their accumulation in nasal polyps and sinus mucosa in chronic rhinosinusitis, supporting a potential role in asthma and CRSwNP. It also says increased innate type 2 responses were not observed in allergic rhinitis (AR). The Discussion suggests ILC2s may play a more prominent role in asthma than in AR, so the source does not support treating all three diseases alike. (LLM, conf=0.88)
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- Authors
- Bartemes, K. R., Kephart, G. M., Fox, S. J., & Kita, H.
- Affiliations
- Mayo Clinic (US)
- Corresponding author
- Kita, Hirohito
- Venue
- Journal of Allergy and Clinical Immunology
- Publisher
- Elsevier BV
- Published
- 2014-09
- Volume
- 134
- Issue
- 3
- Pages
- 671-678.e4
- ISSN
- 0091-6749 (ISSN-L)
- PMC
- PMC4149890
- OpenAlex
- W1969324457
- Cited by
- 390
- References
- 44
- Citations by year
- 2026: 18 · 2025: 16 · 2024: 22 · 2023: 30 · 2022: 29 · 2021: 40 · 2020: 36 · 2019: 33 · 2018: 30 · 2017: 53 · 2016: 40 · 2015: 38 · 2014: 5
- FWCI
- 20.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Allergy and Clinical Immunology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (AI34486, HL117823)
- Mayo Foundation for Medical Education and Research
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › IL-33, ST2, and ILC Pathways
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Keywords
- ILC2, allergic asthma, allergic rhinitis, IL-33, IL-25, IL-5, IL-13, IL-4, peripheral blood mononuclear cells, CRTH2, CD127, thymic stromal lymphopoietin, IL-7, immunopathogenesis
- MeSH terms
- Asthma, Blood Circulation, Cell Differentiation, Cell Separation, Cells, Cultured, Female, Flow Cytometry, Humans, Immunity, Innate, Interleukins, Lymphocytes, Male, Middle Aged, Receptors, Immunologic, Receptors, Prostaglandin, Th2 Cells, Interleukin-7 Receptor alpha Subunit, Rhinitis, Allergic
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