The DOI matches its Crossref record; 5 of the 6 references it cites were found in a registry or an index.
Protein quantification and its tolerance for different interfering reagents using the BCA-method with regard to 2D SDS PAGE
Abstract
Measuring the protein content of a sample is a mandatory and frequently practiced procedure in the lab. Although the procedure is quite simple and convenient to perform with commercially available kits, incompatible reagents in the lysate can cause problems in the quality of measurement. Unfortunately these reagents are cornerstones of high efficiency lysing buffers, e.g. high amounts of urea or beta-mercaptoethanol. In this study we addressed the tolerance of the well-known BCA-assay (bicinchoninic acid) to various reagents in different concentrations, with special regard to a subsequent 2D-gelelectrophoresis. As a result, the kit is incompatible with the recipes of regular 2D-buffers. Also, when mixing two different reagents interfering effects will occur in a non-predictable way. Therefore we established a new method to quantify protein content in lysates ready for 2D-gelelectrophoresis: by mixing an aliquot with SDS, an equilibration is performed to that the sample can be run on a regular 1D SDS PAGE. Image analysis following fluorescence staining (SYPRO Ruby) reveals the absolute protein content in comparison to a BSA dilution curve processed accordingly.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The methodology was validated considering the tolerance criteria described by Krieg et al. [16].”
From Functional and Bioactive Characterization of Hemp Cake Proteins and Polyphenols from Non-Psychoactive Cannabis sativa (2025) · cites this paper as Krieg et al. [16]
Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 316,394
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Measurement of protein using bicinchoninic acid
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) · Analytical Biochemistry · doi:10.1016/0003-2697(85)90442-7 · Cited by 18,482
Leak, L. V., Liotta, L. A., Krutzsch, H. C., Jones, M., Fusaroa, V. A., Ross, S., Zhao, Y., & Petricoin, E. F. (2004) · PROTEOMICS · doi:10.1002/pmic.200300573 · Cited by 80
Dilution of protein gel stain results in retention of staining capacity
Krieg, R. C., Paweletz, C. P., Liotta, L. A., & Petricoin, E. F. (2003) · BioTechniques · doi:10.2144/03352pt01 · Cited by 16
Self-citation
Cited by
Sorted by citations
An overview of technical considerations for Western blotting applications to physiological research
Bass, J. J., Wilkinson, D. J., Rankin, D., Phillips, B. E., Szewczyk, N. J., Smith, K., & Atherton, P. J. (2016) · Scandinavian Journal of Medicine and Science in Sports · doi:10.1111/sms.12702 · Cited by 433
Erkekoğlu, P., Rachidi, W., Gürsoy-Yüzügüllü, Ö., Giray, B., Favier, A. E., Öztürk, M., & Hıncal, F. (2010) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2010.07.016 · Cited by 207
Cortés‐Ríos, J., Zárate, A. M., Figueroa, J. D., Medina, J., Fuentes-Lemus, E., Rodriguez-Fernández, M., Aliaga, M. E., & López‐Alarcón, C. (2020) · Analytical Biochemistry · doi:10.1016/j.ab.2020.113904 · Cited by 181
Chitosan–hyaluronic acid hybrid film as a novel wound dressing: in vitro and in vivo studies
Hai-tang, X., Ma, L., Shi, H., Gao, C., & Han, C. (2007) · Polymers for Advanced Technologies · doi:10.1002/pat.906 · Cited by 164
Protein extraction from activated sludge: An analytical approach
Ras, M., Girbal‐Neuhauser, E., Paul, E., Spérandio, M., & Cavaillé-Lefebvre, D. (2007) · Water Research · doi:10.1016/j.watres.2007.11.011 · Cited by 154
Lü, J., Jun-Ye, M. I. A. O., Su, T., Liu, Y., & He, R. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2013.04.028 · Cited by 132
An improved method to directly estimate cellulase adsorption on biomass solids
Kumar, R., & Wyman, C. E. (2008) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2007.12.005 · Cited by 123
Wang, X., Wang, X., Zhao, J., Song, J., Wang, J., Ma, R., & Ma, J. (2017) · Chemical Engineering Journal · doi:10.1016/j.cej.2017.03.062 · Cited by 114
Zuriaga-Agustí, E., Bes-Piá, A., Mendoza‐Roca, J. A., & Alonso, J. L. (2013) · Separation and Purification Technology · doi:10.1016/j.seppur.2013.03.048 · Cited by 105
Zhang, J., Ren, A., Chen, H., Zhao, M., Shi, L., Chen, M., Wang, H., & Feng, Z. (2015) · PLoS ONE · doi:10.1371/journal.pone.0123025 · Cited by 83
Li, W., Wang, Y., Wei, H., Zhang, Y., Guo, Z., Qiu, Y., Wen, L., & Xie, Z. (2020) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.10613 · Cited by 74
Wang, L., Wang, L., Su, C., Wen, C., Gong, Y., You, Y., Zhao, J., Han, Y., Song, S., & Xiao, H. (2020) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2020.116812 · Cited by 73
Erkekoğlu, P., Rachidi, W., Rosa, V. D., Giray, B., Favier, A. E., & Hıncal, F. (2010) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2010.04.038 · Cited by 73
Rosa, V. D., Erkekoğlu, P., Forestier, A., Favier, A. E., Hıncal, F., Diamond, A. M., Douki, T., & Rachidi, W. (2011) · Free Radical Research · doi:10.3109/10715762.2011.647009 · Cited by 65
Yun, S., Choi, C.-W., Kwon, S., Park, G. W., Cho, K., Kwon, K., Kim, J. Y., Yoo, J. S., Lee, J. C., Choi, J., et al. (2010) · Journal of Proteome Research · doi:10.1021/pr101012s · Cited by 57
Zhao, D., Dai, W., Tao, H., Zhuang, W., Qu, M., & Chang, Y. (2020) · Journal of Food Science · doi:10.1111/1750-3841.15319 · Cited by 57
Yun, S., Choi, C.-W., Park, S.-H., Lee, J. C., Leem, S., Choi, J., Kim, S., & Kim, S. I. (2008) · The Journal of Microbiology · doi:10.1007/s12275-008-0202-3 · Cited by 54
Isolation and Detection Technologies of Extracellular Vesicles and Application on Cancer Diagnostic
Ma, C., Jiang, F., Ma, Y., Wang, J., Li, H., & Zhang, J. (2019) · Dose-Response · doi:10.1177/1559325819891004 · Cited by 58
Huang, Y., He, T.-B., Cuan, X.-D., Wang, X., Hu, J., & Sheng, J. (2018) · Molecules · doi:10.3390/molecules23102658 · Cited by 52
Xu, X., Quan, L., & Shen, M. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2015.03.013 · Cited by 46
Akbarzadeh-Khiavi, M., Safary, A., Barar, J., Farzi-Khajeh, H., Barzegari, A., Mousavi, R., Somi, M. H., & Omidi, Y. (2019) · Bioimpacts · doi:10.15171/bi.2020.04 · Cited by 43
Yuan, H., Qian, B., Zhang, W., & Lan, M. (2015) · Applied Surface Science · doi:10.1016/j.apsusc.2015.12.072 · Cited by 40
Wang, S., Mou, Z.-Z., Ma, Y., Li, J., Li, J., Ji, X., Wu, K., Li, L., Lu, W., & Zhou, T. (2015) · Biochemical Pharmacology · doi:10.1016/j.bcp.2015.03.013 · Cited by 39
Structural Characteristics and Properties of Silk Fibroin/Poly(lactic acid) Blend Films
Zhu, H., Feng, X., Zhang, H.-P., Guo, Y., Zhang, J., & Chen, J. (2009) · Journal of Biomaterials Science Polymer Edition · doi:10.1163/156856209x452980 · Cited by 36
Vilcacundo, R., Barrio, D. A., Carpio, C., García‐Ruiz, A., Ruales, J., Hernández‐Ledesma, B., & Carrillo, W. (2017) · Plant Foods for Human Nutrition · doi:10.1007/s11130-017-0626-1 · Cited by 39
Qiao, M., Tu, M., Chen, H., Mao, F., Yu, C., & Du, M. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19072100 · Cited by 34
Li, Y., Deng, B., Ouyang, N., Yuan, P., Zheng, L., & Wang, W. (2017) · Journal of Assisted Reproduction and Genetics · doi:10.1007/s10815-017-0929-z · Cited by 32
The Effects of Polymer Coating of Gold Nanoparticles on Oxidative Stress and DNA Damage
Sen, G. T., Özkemahlı, G., Shahbazi, R., Erkekoğlu, P., Ulubayram, K., & Kocer-Gumusel, B. (2020) · International Journal of Toxicology · doi:10.1177/1091581820927646 · Cited by 31
Effect of manganese on the resistance of tomato to Pseudocercospora fuligena
Heine, G., Max, J. F. J., Führs, H., Puente, D. W. M., Heintz, D., & Horst, W. J. (2011) · Journal of Plant Nutrition and Soil Science · doi:10.1002/jpln.201000440 · Cited by 30
Proteins involved in responses to biofilm and planktonic modes in Cronobacter sakazakii
Ye, Y., Jiao, R., Gao, J., Li, H., Ling, N., Wu, Q., Zhang, J., & Xu, X. (2015) · LWT · doi:10.1016/j.lwt.2015.09.039 · Cited by 30
Human salivary protein extraction from RNAPro·SAL™, Pure·SAL™, and passive drooling method
Khurshid, Z., Moin, S. F., Khan, R. S., Agwan, M. A. S., Alwadaani, A. H., & Zafar, M. S. (2017) · European Journal of Dentistry · doi:10.4103/ejd.ejd_183_17 · Cited by 30
Coptisine suppresses tumor growth and progression by down-regulating MFG-E8 in colorectal cancer
Cao, Q., Hong, S., Li, Y., Chen, H., Shen, Y., Shao, K., Lu, M., Dai, H., Ma, S., & Dai, G. (2018) · RSC Advances · doi:10.1039/c8ra05806g · Cited by 30
Hydrolytic Enzyme Protease in Sludge: Recovery and Its Application
Karn, S. K., & Kumar, A. (2015) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-015-0161-6 · Cited by 27
Li, J., Jin, H., Razzak, M. A., Kim, E. J., & Choi, S. S. (2022) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-021-0243-6 · Cited by 28
Ye, Y., Gao, J., Jiao, R., Li, H., Wu, Q., Zhang, J., & Zhong, X. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.01238 · Cited by 27
Reduction of nitric oxide catalyzed by hydroxylamine oxidoreductase from an anammox bacterium
Irisa, T., Hira, D., Furukawa, K., & Fujii, T. (2014) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2014.05.018 · Cited by 27
Characterization of sericin protein recovered from silk wastewaters
Çapar, G., & Aygün, S. S. (2015) · Turkish Bulletin of Hygiene and Experimental Biology · doi:10.5505/turkhijyen.2015.47113 · Cited by 25
Enhancing the potential production of bioethanol with bamboo by γ-valerolactone/water pretreatment
Zhan, Y., Wang, M., Ma, T., & Li, Z. (2022) · RSC Advances · doi:10.1039/d2ra02421g · Cited by 24
A fluorescence anisotropy method for measuring protein concentration in complex cell culture media
Groza, R. C., Calvet, A., & Ryder, A. G. (2014) · Analytica Chimica Acta · doi:10.1016/j.aca.2014.03.007 · Cited by 24
Ferrer-Polonio, E., Fernández-Navarro, J., Alonso, J. L., Amorós, I., Bes-Piá, A., & Mendoza‐Roca, J. A. (2017) · Journal of Environmental Management · doi:10.1016/j.jenvman.2017.08.009 · Cited by 24
Du, J., Zhu, Z., Yang, J., Wang, J., & Jiang, X. (2021) · Heritage Science · doi:10.1186/s40494-021-00519-y · Cited by 22
Polymeric Nanoparticles as a Self‐Adjuvanting Peptide Vaccine Delivery System: The Role of Shape
Koirala, P., Chen, S. R., Boer, J. C., Khalil, Z. G., Deceneux, C., Goodchild, G., Lu, L., Faruck, M. O., Shalash, A. O., Bashiri, S., et al. (2023) · Advanced Functional Materials · doi:10.1002/adfm.202209304 · Cited by 23
Gu, Y., Chen, K., Xi, B., Xie, J., & Bing, X. (2022) · Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology · doi:10.1016/j.cbpc.2022.109339 · Cited by 23
Targeting neuronal nitric oxide synthase by a cell penetrating peptide Tat-LK15/siRNA bioconjugate
Peng, J., Rao, Y., Yang, X. W., Jia, J., Wu, Y., Jianhua, L., Tao, Y., & Tu, W. (2017) · Neuroscience Letters · doi:10.1016/j.neulet.2017.04.045 · Cited by 21
Sun, Y., Gao, G., & Cai, T. (2021) · Bioengineered · doi:10.1080/21655979.2021.1972781 · Cited by 20
Peng, Y., Jiang, H., Wu, Y., Zhang, J., & Chen, Y. (2022) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2022.104190 · Cited by 20
Ferrer-Polonio, E., White, K., Mendoza‐Roca, J. A., & Bes-Piá, A. (2018) · Journal of Environmental Management · doi:10.1016/j.jenvman.2018.09.036 · Cited by 20
Bachmann, S., Bakry, R. S., Huck, C. W., Polato, F., Corradini, D., & Bonn, G. K. (2011) · Electrophoresis · doi:10.1002/elps.201000653 · Cited by 19
Özkemahlı, G., Erkekoğlu, P., Ercan, A., Zeybek, N. D., Yersal, N., & Kocer-Gumusel, B. (2022) · Environmental Science and Pollution Research · doi:10.1007/s11356-022-22937-6 · Cited by 19
Xie, P., Horio, F., Fujii, I., Zhao, J.-E., Shinohara, M., & Matsukura, M. (2018) · International Journal of Oncology · doi:10.3892/ijo.2018.4297 · Cited by 19
Zang, L., Zhang, Q., Zhou, Y. J., Zhao, Y. P., Lu, L., Zhou, J., Peng, Z., & Zou, S. (2016) · Systems Biology in Reproductive Medicine · doi:10.3109/19396368.2016.1164260 · Cited by 18
Scaffold functionalization to support a tissue biocompatibility
Carmagnola, I., Ranzato, E., & Chiono, V. (2018) · Elsevier eBooks · doi:10.1016/b978-0-08-100979-6.00011-2 · Cited by 17
Cui, L., Bai, Y., Zhang, J., Zhang, S., & Xu, J. (2016) · Clinical and Experimental Hypertension · doi:10.3109/10641963.2016.1163366 · Cited by 18
Naïja, A., Kestemont, P., Chénais, B., Haouas, Z., Blust, R., Helal, A. N., & Marchand, J. (2017) · Chemosphere · doi:10.1016/j.chemosphere.2017.11.118 · Cited by 18
Amaral, R., Concha, T., Vítor, J. M. B., Almeida, A. J., Calado, C. R. C., & Gonçalves, L. (2025) · Pharmaceutics · doi:10.3390/pharmaceutics17010132 · Cited by 17
Erkekoğlu, P., Chao, M., Ye, W., Ge, J., Trudel, L. J., Skipper, P. L., Giray, B., Engelward, B. P., Wogan, G. N., & Tannenbaum, S. R. (2014) · Food and Chemical Toxicology · doi:10.1016/j.fct.2014.06.031 · Cited by 17
THE COMPOSITION OF SUGARCANE JUICES DERIVED FROM BURNT CANE AND WHOLE GREEN CANE CROP
Thai, C. C. D., & Doherty, W. O. S. (2011) · QUT ePrints (Queensland University of Technology) · Cited by 16
Phenanthrene degradation by the bacterium Pseudomonas stutzeri JP1 under low oxygen condition
Kong, J., Wang, H., Liang, L., Li, L., Xiong, G., & Hu, Z. (2017) · International Biodeterioration & Biodegradation · doi:10.1016/j.ibiod.2017.06.001 · Cited by 16
Chao, M., Erkekoğlu, P., Tseng, C., Ye, W., Trudel, L. J., Skipper, P. L., Tannenbaum, S. R., & Wogan, G. N. (2014) · Journal of Applied Toxicology · doi:10.1002/jat.3046 · Cited by 15
Cadmium exposure exerts neurotoxic effects in peacock blennies Salaria pavo
Naïja, A., Kestemont, P., Chénais, B., Haouas, Z., Blust, R., Helal, A. N., & Marchand, J. (2017) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2017.05.041 · Cited by 15
Gu, P., Wu, H., Zhang, Z., Li, Q., Zhang, W., Zheng, Z., Yang, K., Miao, H., & Xu, J. (2022) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2022.132256 · Cited by 15
Yong, J. M., Hakobyan, K., Xu, J., Mellick, A. S., Whitelock, J. M., & Liang, K. (2023) · Biotechnology Journal · doi:10.1002/biot.202300015 · Cited by 18
Su, H., Xue, Z., Feng, Y., Xie, Y., Deng, B., Yao, Y., Tian, X., An, Q., Yang, L., Yao, Q., et al. (2019) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2019.114789 · Cited by 14
Antioxidant dihydrolipolic acid protects against in vitro aluminum‐induced toxicity
Sanajou, S., Yirün, A., Demirel, G., Çakır, D. A., Şahin, G., Erkekoğlu, P., & Baydar, T. (2023) · Journal of Applied Toxicology · doi:10.1002/jat.4513 · Cited by 14
Corbatón‐Báguena, M., Gugliuzza, A., Cassano, A., Mazzei, R., & Giorno, L. (2015) · Journal of Membrane Science · doi:10.1016/j.memsci.2015.03.061 · Cited by 14
Liao, Z.-C., Guan, W., Chen, F., Hou, D., Wang, C.-X., Lv, Y.-T., Qiao, H., Chen, J., & Han, J. (2014) · Journal of Animal and Feed Sciences · doi:10.22358/jafs/65704/2014 · Cited by 13
Peng, Y., Jiang, H., Zhang, J., Wu, Y., Zhang, J., Zhou, J., & Chen, Y. (2022) · Journal of Food Measurement & Characterization · doi:10.1007/s11694-022-01483-2 · Cited by 12
Cui, W., Xue, H., Cheng, H., Zhang, H., Jin, J., & Wang, Q. (2018) · Electrophoresis · doi:10.1002/elps.201800430 · Cited by 11
Effect of cadmium in the gonads of mussel (Mytilus coruscus): an ionomics and proteomics study
Meng, C., Wang, K., Zhang, X., & Zhu, X. (2023) · Environmental Science and Pollution Research · doi:10.1007/s11356-023-27208-6 · Cited by 11
Cho, K., Kim, M.-U., Jeong, G., Khan, F., Jo, D., & Kim, Y. (2024) · Foods · doi:10.3390/foods13030366 · Cited by 11
Zhong, M., Hu, C., Ren, C., Luo, X., & Cai, Y. (2015) · International Journal of Food Properties · doi:10.1080/10942912.2015.1076456 · Cited by 10
Fan, Y., Zheng, Y., Chen, L., Xu, L., da Silva, J. A. T., Wu, B., & Yu, X. (2023) · Scientia Horticulturae · doi:10.1016/j.scienta.2023.111859 · Cited by 10
Wang, J., Xi, Y., Sun, B., Deng, J., & Ai, N. (2024) · Food Chemistry X · doi:10.1016/j.fochx.2024.101187 · Cited by 10
Bi, Z., Yang, X., Yu, W., Shu, J., & Zhang, Y. (2014) · PLoS ONE · doi:10.1371/journal.pone.0090435 · Cited by 10
Protein–protein binding affinities by pulse proteolysis: Application to TEM‐1/BLIP protein complexes
Hanes, M. S., Ratcliff, K., Marqusee, S., & Handel, T. M. (2010) · Protein Science · doi:10.1002/pro.467 · Cited by 10
Biomarkers assessment in the peacock blenny Salaria pavo exposed to cadmium
Naïja, A., Marchand, J., Kestemont, P., Haouas, Z., Blust, R., Chénais, B., & Helal, A. N. (2016) · Environmental Science and Pollution Research · doi:10.1007/s11356-016-6754-6 · Cited by 10
Mecozzi, M., Pietroletti, M., & Conti, M. E. (2008) · International Journal of Environment and Pollution · doi:10.1504/ijep.2008.018416 · Cited by 10
Zhang, X., Xue, H., Khan, A., Jia, P., Kong, X., Li, L., & Zhang, Z. (2021) · Industrial Crops and Products · doi:10.1016/j.indcrop.2021.114094 · Cited by 10
Naïja, A., Marchand, J., Kestemont, P., Haouas, Z., Blust, R., Chénais, B., & Helal, A. N. (2016) · Environmental Science and Pollution Research · doi:10.1007/s11356-016-7401-y · Cited by 9
Du, J., Zhu, Z., Dong, J., Lu, Z., Zhao, X., & Yang, J. (2023) · Journal of Cultural Heritage · doi:10.1016/j.culher.2023.05.024 · Cited by 8
Antioxidants and selenocompounds inhibit 3,5-dimethylaminophenol toxicity to human urothelial cells
Erkekoğlu, P., Chao, M., Tseng, C., Engelward, B. P., Köse, Ö., Giray, B., Wogan, G. N., & Tannenbaum, S. R. (2019) · Archives of Industrial Hygiene and Toxicology · doi:10.2478/aiht-2019-70-3159 · Cited by 8
HDAC3 Knockdown Dysregulates Juvenile Hormone and Apoptosis-Related Genes in Helicoverpa armigera
Chang, H., Xu, Z., Li, W., Cai, C., Wang, W., Ge, P., Jia, X., Li, Y., Ding, T., Ma, W., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232314820 · Cited by 8
Sanajou, S., Yirün, A., Demirel, G., Erkekoğlu, P., Şahin, G., & Baydar, T. (2024) · Journal of Applied Toxicology · doi:10.1002/jat.4695 · Cited by 7
Liu, H., Gao, Z., Cui, S., Xia, X., Hou, C., Jiang, Z.-M., Zhao, C., Wang, C.-B., Chen, S.-G., & Qu, X. (2011) · Food Biophysics · doi:10.1007/s11483-011-9214-3 · Cited by 7
Enhanced release of bone morphogenetic proteins from demineralized bone matrix by gamma irradiation
Sung, N., & Choi, J. (2015) · Radiation Physics and Chemistry · doi:10.1016/j.radphyschem.2015.02.012 · Cited by 7
Zeybek, N. D., Sur, Ü., Oflaz, O., Erkekoğlu, P., Balcı, A., Özkemahlı, G., Aşçı, A., Kızılgün, M., Edebal, O. H., & Giray, B. (2020) · Archives of Industrial Hygiene and Toxicology · doi:10.2478/aiht-2020-71-3360 · Cited by 6
Ferrer-Polonio, E., Fernández-Navarro, J., Alonso, J. L., Bes-Piá, A., & Mendoza‐Roca, J. A. (2018) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.5689 · Cited by 6
Deciphering cargo contents in extracellular vesicles of Candida haemulonii var. vulnera
Oliveira, B., Bitencourt, T. A., dos Santos, P. W. D. S., Pagano, A. D., Pessoni, A. M., Rezende, C. P., Piraine, R. E. A., Masson, A. P., Faça, V. M., Campos, V. F., et al. (2025) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2025.04.014 · Cited by 5
Griffin, L. E., Fausnacht, D. W., Tuzo, J., Addington, A. K., Racine, K. C., Zhang, H., Hughes, M. D., England, K. M., Bruno, R. S., O’Keefe, S. F., et al. (2019) · Nutrition Research · doi:10.1016/j.nutres.2019.03.011 · Cited by 4
GASTROINTESTINAL DIGESTION OF KAHAI PROTEIN CONCENTRATE (CARYODENDRON ORINOCENSE KARST)
Greffa, J., Barrionuevo, A., Vilcacundo, E., & Carrillo, W. (2018) · Asian Journal of Pharmaceutical and Clinical Research · doi:10.22159/ajpcr.2018.v11i6.20374 · Cited by 3
Wu, Z., Wang, Z., Li, Z., Hao, H., Qi, Y., & Feng, D. (2023) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2023.115971 · Cited by 3
Fucoidan alleviates the inhibition of protein digestion by chitosan and its oligosaccharides
Wang, L., Wang, L., Wang, N., Chen, S., Wen, C., Yan, C., & Song, S. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.132072 · Cited by 4
Wang, L., Wang, L., Cao, C., Zhao, J., Song, C., Bao, Z., Yan, C., & Song, S. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.127072 · Cited by 3
Flaherty, R. A., & Lee, S. W. (2016) · Journal of Visualized Experiments · doi:10.3791/54406 · Cited by 3
Functional Properties of Protein and Chitin from Commercial Cricket Flour
Hirsch, A. J. (2019) · Purdue e-Pubs (Purdue University System) · doi:10.25394/pgs.7479296 · Cited by 3
Chen, Z., Li, J., Zhou, Z., Wang, Y., Li, M., Li, L., & Ma, S. (2026) · Food Research International · doi:10.1016/j.foodres.2026.118690 · Cited by 2
Preparation and characterization of high‐molecular‐weight sericin by γ irradiation
Sung, N., Byun, E., Kwon, S., Yoo, Y., Jung, P., Kim, J., Choi, J., Kim, M. R., & Lee, J.-W. (2010) · Journal of Applied Polymer Science · doi:10.1002/app.33332 · Cited by 2
Song, T., Cheng, J., Diao, X., Yang, M., Chen, B., Zhang, H., Elango, J., & Wu, W. (2024) · Fermentation · doi:10.3390/fermentation10090468 · Cited by 2
Kaur, P., Pan, H., Longley, M. J., Copeland, W. C., & Wang, H. (2021) · BIO-PROTOCOL · doi:10.21769/bioprotoc.4139 · Cited by 2
Metadata
- Authors
- Krieg, R. C., Dong, Y., Schwamborn, K., & Knuechel, R.
- Affiliations
- RWTH Aachen University (DE)
- Venue
- Journal of Biochemical and Biophysical Methods
- Publisher
- Elsevier BV
- Published
- 2005-10
- Volume
- 65
- Issue
- 1
- Pages
- 13-19
- ISSN
- 0165-022X (ISSN-L)
- OpenAlex
- W2068769109
- Cited by
- 122
- References
- 6
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 6 · 2023: 9 · 2022: 9 · 2021: 5 · 2020: 6 · 2019: 6 · 2018: 9 · 2017: 11 · 2016: 6 · 2015: 12 · 2014: 7 · 2013: 3 · 2012: 1
- FWCI
- 0.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biochemical and Biophysical Methods (publishedVersion)
- PubMed (publishedVersion)
- RWTH Publications (RWTH Aachen) (submittedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- protein quantification, bicinchoninic acid assay, urea, 2-mercaptoethanol
- MeSH terms
- Proteins, Quinolines, Reagent Kits, Diagnostic, Sensitivity and Specificity, Electrophoresis, Gel, Two-Dimensional, Reproducibility of Results
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review