Preparation of immunogen-reduced and biocompatible extracellular matrices from porcine liver
Abstract
Decellularized biologic matrices are plausible biomedical materials for the bioengineering in liver transplantation. However, one of the concerns for safe medical application is the lack of objective assessment of the immunogen within the materials and the in vivo immune responses to the matrices. The purpose of this study was the production of immunogen-reduced and biocompatible matrices from porcine liver. In the present study, 0.1% SDS solution was effective for removing DNA fragments and sequences encoding possible immunogenic and viral antigens within the matrices. The PCR analysis showed that galactose-α-1,3 galactose β-1,4-N-acetylglucosamine (1,3 gal), swine leukocyte antigen (SLA), and porcine endogenous retrovirus (PERV) were completely removed in the matrices. Collagen and glycosaminoglycans (GAGs) were preserved over 63%-71%, respectively, compared to those of native liver. The implanted decellularized tissues showed minimal host responses and naturally degraded within 10 weeks. In this study, we produced immunogen-reduced and biocompatible extracellular matrices from porcine liver. Although future investigations would be required to determine the mechanism of the host reaction, this study could provide useful information of porcine liver-derived biologic matrices for liver researches.
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Decellularization has demonstrated efficient capability in removing or reducing the alpha-gal content from porcine liver.
“Decellularization has demonstrated efficient capability in removing or reducing the alpha-gal content from porcine liver [[67], [68], [69]].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [68]
Upon subcutaneous implantation in pigs, Park et al.'s implanted decellularized tissues elicited minimal host responses and naturally degraded within 10 weeks.
“Upon subcutaneous implantation in pigs, the implanted decellularized tissues elicited minimal host responses and naturally degraded within 10 weeks.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [68]
Park et al. reported that 0.1% SDS effectively removed DNA and potential immunogenic and viral antigens from matrices while preserving 63% of collagen and 71% of GAGs compared to native liver.
“They reported that 0.1 % SDS effectively removed DNA and potential immunogenic and viral antigens from matrices while preserving 63 % of collagen and 71 % of GAGs compared to native liver.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [68]
Cites
The use of whole organ decellularization for the generation of a vascularized liver organoid
Baptista, P. M., Siddiqui, M. M., Lozier, G., Rodriguez, S. R., Atala, A., & Soker, S. (2011) · Hepatology · doi:10.1002/hep.24067 · Cited by 681 · see citing works
Assessing Porcine Liver-Derived Biomatrix for Hepatic Tissue Engineering
Lin, P., Chan, W. C. W., Badylak, S. F., & Bhatia, S. N. (2004) · Tissue Engineering · doi:10.1089/ten.2004.10.1046 · Cited by 236 · see citing works
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Biocompatibility evaluation of tissue-engineered decellularized scaffolds for biomedical application
Hussein, K. H., Park, K.-M., Kang, K.-S., & Woo, H.-M. (2016) · Materials Science and Engineering: C · doi:10.1016/j.msec.2016.05.068 · Cited by 218 · see citing works
Self-citationPark, K.-M., Hussein, K. H., Hong, S.-H., Ahn, C., Yang, S.-R., Park, S.-M., Kweon, O.-K., Kim, B.-M., & Woo, H.-M. (2016) · Tissue Engineering Part A · doi:10.1089/ten.tea.2015.0313 · Cited by 113 · see citing works
Self-citationWang, Y., Bao, J., Wu, X., Wu, Q., Li, Y., Zhou, Y., Li, L., & Bu, H. (2016) · Scientific Reports · doi:10.1038/srep24779 · Cited by 101 · see citing works
Struecker, B., Butter, A., Hillebrandt, K., Polenz, D., Reutzel-Selke, A., Tang, P., Lippert, S., Leder, A., Rohn, S., Geisel, D., et al. (2017) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1948 · Cited by 65 · see citing works
Hussein, K. H., Ahmadzada, B., Correa, J. C., Sultan, A., Wilken, S., Amiot, B., & Nyberg, S. L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.06.001 · Cited by 28 · see citing works
“Several studies have reported the difficulty in completely washing out SDS from decellularized tissues via conventional washing methods due to the strong hydrophobic bonds between SDS and ECM proteins [68,100].”
BackgroundMethodInfluential
- Authors
- Park, K.-M., Park, S.-M., Yang, S.-R., Hong, S.-H., & Woo, H.-M.
- Affiliations
- Kangwon National University (KR)
- Brigham and Women's Hospital (US)
- Corresponding author
- Woo, Heung-Myong
- Venue
- Journal of Bioscience and Bioengineering
- Publisher
- Elsevier BV
- Published
- 2013-02
- Volume
- 115
- Issue
- 2
- Pages
- 207-215
- ISSN
- 1347-4421 (ISSN-L)
- 1389-1723
- OpenAlex
- W2063692778
- Cited by
- 38
- References
- 2
- Citations by year
- 2025: 4 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 5 · 2015: 4 · 2014: 4 · 2013: 2
- FWCI
- 3.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Bioscience and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Rural Development Administration (PJ008030, PJ008032)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Surgery › Xenotransplantation and immune response
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- decellularized extracellular matrix, porcine liver, immunogenicity reduction, swine leukocyte antigen, porcine endogenous retroviruses, collagen, glycosaminoglycans, biocompatibility, liver transplantation, xenotransplantation
- MeSH terms
- Animals, Antigens, Histocompatibility Antigens Class II, Biocompatible Materials, Cell Separation, Collagen, DNA, Extracellular Matrix, Glycosaminoglycans, Graft Rejection, Liver, Transplantation, Heterologous, Histocompatibility Antigens Class I, Liver Transplantation, Endogenous Retroviruses, Tissue Engineering, Sus scrofa, Guided Tissue Regeneration, Tissue Scaffolds
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