Improved rat liver decellularization by arterial perfusion under oscillating pressure conditions: Arterial rat liver decellularization
Abstract
One approach of regenerative medicine to generate functional hepatic tissue in vitro is decellularization and recellularization, and several protocols for the decellularization of livers of different species have been published. This appears to be the first report on rat liver decellularization by perfusion under oscillating pressure conditions, intending to optimize microperfusion and minimize damage to the ECM. Four decellularization protocols were compared: perfusion via the portal vein (PV) or the hepatic artery (HA), with (+P) or without (-P) oscillating pressure conditions. All rat livers (n = 24) were perfused with 1% Triton X-100 and 1% sodium dodecyl sulphate, each for 90 min with a perfusion rate of 5 ml/min. Perfusion decellularization was observed macroscopically and the decellularized liver matrices were analysed by histology and biochemical analyses (e.g. levels of DNA, glycosaminoglycans and hepatocyte growth factor). Livers decellularized via the hepatic artery and under oscillating pressure showed a more homogeneous decellularization and less remaining DNA, compared with the livers of the other experimental groups. The novel decellularization method described is effective, quick (3 h) and gentle to the extracellular matrix and thus represents an improvement of existing methodology. Copyright © 2014 John Wiley & Sons, Ltd.
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The efficiency of decellularization via perfusion depends on the route of perfusion, perfusion direction, perfusion conditions, duration, type and concentration of decellularizing agents, and the size of the liver.
“Importantly, the efficiency of decellularization via perfusion depends on various factors, including the route of perfusion (hepatic artery or portal vein or inferior vena cava), perfusion direction (antegrade or retrograde), perfusion conditions (controlled pressure and flow rate), duration of the perfusion, type and concentration of decellularizing agents, and the size of the liver [7,26,170].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [170]
The biliary network tree is confirmed to be intact after decellularization by scan electron microscopy, corrosion cast, dye perfusion, and contrast radiography; only reconstruction by bile duct cells is necessary.
“Fortunately, the biliary network tree is confirmed to be intact after decellularization by scan electron microscopy, corrosion cast, dye perfusion, and contrast radiography; only reconstruction by bile duct cells is necessary [12,110,170,198].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [170]
Cites
1 retracted
RETRACTED: Engineered whole organs and complex tissues
Badylak, S. F., Weiss, D. J., Caplan, A., & Macchiarini, P. (2012) · The Lancet · doi:10.1016/s0140-6736(12)60073-7 · Cited by 417 · see citing works
RetractedAn overview of tissue and whole organ decellularization processes
Crapo, P. M., Gilbert, T. W., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.01.057 · Cited by 3,591 · see citing works
Farndale, R. W., Buttle, D. J., & Barrett, A. J. (1986) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(86)90306-5 · Cited by 3,313 · see citing works
Uygun, B. E., Soto-Gutierrez, A., Yagi, H., Izamis, M.-L., Guzzardi, M. A., Shulman, C., Milwid, J., Kobayashi, N., Tilles, A., Berthiaume, F., et al. (2010) · Nature Medicine · doi:10.1038/nm.2170 · Cited by 1,370 · see citing works
Badylak, S. F., Taylor, D., & Uygun, K. (2011) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071910-124743 · Cited by 1,041 · see citing works
Autoimmunity and the Clearance of Dead Cells
Nagata, S., Hanayama, R., & Kawane, K. (2010) · Cell · doi:10.1016/j.cell.2010.02.014 · Cited by 877 · see citing works
The use of whole organ decellularization for the generation of a vascularized liver organoid
Baptista, P. M., Siddiqui, M. M., Lozier, G., Rodriguez, S. R., Atala, A., & Soker, S. (2011) · Hepatology · doi:10.1002/hep.24067 · Cited by 681 · see citing works
Extracellular Matrix Molecules: Potential Targets in Pharmacotherapy
Järveläinen, H., Sainio, A., Koulu, M., Wight, T. N., & Penttinen, R. P. K. (2009) · Pharmacological Reviews · doi:10.1124/pr.109.001289 · Cited by 566 · see citing works
Zheng, M., Chen, J., Kirilak, Y., Willers, C., Xu, J., & Wood, D. (2005) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.30170 · Cited by 440 · see citing works
Organ transplantation—how much of the promise has been realized?
Lechler, R. I., Sykes, M., Thomson, A. W., & Turka, L. A. (2005) · Nature Medicine · doi:10.1038/nm1251 · Cited by 390 · see citing works
Cortiella, J., Niles, J. A., Cantu, A., Brettler, A. C., Pham, A. N., Vargas, G., Winston, S., Wang, J. P., Walls, S., & Nichols, J. E. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0730 · Cited by 381 · see citing works
Embryonic Stem Cells Proliferate and Differentiate when Seeded into Kidney Scaffolds
Ross, E. A., Williams, M. J., Hamazaki, T., Terada, N., Clapp, W. L., Adin, C. A., Ellison, G. W., Jorgensen, M. J., & Batich, C. D. (2009) · Journal of the American Society of Nephrology · doi:10.1681/asn.2008111196 · Cited by 380 · see citing works
A Whole-Organ Regenerative Medicine Approach for Liver Replacement
Soto-Gutierrez, A., Zhang, L., Medberry, C., Fukumitsu, K., Faulk, D., Jiang, H., Reing, J., Gramignoli, R., Komori, J., Ross, M., et al. (2011) · Tissue Engineering Part C: Methods · doi:10.1089/ten.tec.2010.0698 · Cited by 311 · see citing works
Maintenance of Human Hepatocyte Function In Vitro by Liver-Derived Extracellular Matrix Gels
Sellaro, T. L., Ranade, A. R., Faulk, D. M., McCabe, G. P., Dorko, K., Badylak, S. F., & Strom, S. C. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0587 · Cited by 276 · see citing works
Liver Transplantation After Organ Preservation With Normothermic Extracorporeal Perfusion
Schön, M. R., Kollmar, O., Wolf, S., Schrem, H., Matthes, M., Akkoc, N., Schnoy, N., & Neuhaus, P. (2001) · Annals of Surgery · doi:10.1097/00000658-200101000-00017 · Cited by 265 · see citing works
Self-citationWang, Y., Cui, C.-B., Yamauchi, M., Miguez, P., Roach, M., Malavarca, R., Costello, J. M., Cardinale, V., Wauthier, E., Barbier, C., et al. (2011) · Hepatology · doi:10.1002/hep.24012 · Cited by 220 · see citing works
Use of Decellularized Porcine Liver for Engineering Humanized Liver Organ
Barakat, O., Abbasi, S., Rodriguez, G., Rios, J., Wood, R. P., Ozaki, C., Holley, L. S., & Gauthier, P. K. (2012) · Journal of Surgical Research · doi:10.1016/j.jss.2011.09.033 · Cited by 208 · see citing works
Dynamic reciprocity: how do extracellular matrix and hormones direct gene expression?
Bissell, M. J., & Aggeler, J. (1987) · PubMed · Cited by 192 · see citing works
Human-Scale Whole-Organ Bioengineering for Liver Transplantation: A Regenerative Medicine Approach
Yagi, H., Fukumitsu, K., Fukuda, K., Kitago, M., Shinoda, M., Obara, H., Itano, O., Kawachi, S., Tanabe, M., Coudriet, G. M., et al. (2013) · Cell Transplantation · doi:10.3727/096368912x654939 · Cited by 188 · see citing works
Stern, M. M., Myers, R. L., Hammam, N., Stern, K. A., Eberli, D., Kritchevsky, S. B., Söker, S., & Dyke, M. E. V. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2008.12.069 · Cited by 185 · see citing works
Method for the decellularization of intact rat liver
Shupe, T. D., Williams, M. J., Brown, A. R., Willenberg, B., & Petersen, B. E. (2010) · Organogenesis · doi:10.4161/org.6.2.11546 · Cited by 175 · see citing works
Liver support strategies: cutting-edge technologies
Struecker, B., Raschzok, N., & Sauer, I. M. (2013) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2013.204 · Cited by 165 · see citing works
Self-citationLiver Transplantation: The Current Situation
Adam, R. A., & Hoti, E. (2009) · Seminars in Liver Disease · doi:10.1055/s-0029-1192052 · Cited by 134 · see citing works
Bao, J., Shi, Y., Sun, H., Yin, X., Yang, R., Li, L., Chen, X., & Bu, H. (2011) · Cell Transplantation · doi:10.3727/096368910x536572 · Cited by 133 · see citing works
Ji, R., Zhang, N., You, N., Li, Q., Liu, W., Jiang, N., Liu, J., Zhang, H., Wang, D., Tao, K., et al. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.08.058 · Cited by 129 · see citing works
Ren, H., Shi, X., Tao, L., Xiao, J., Han, B., Zhang, Y., Yuan, X., & Ding, Y. (2013) · Liver International · doi:10.1111/liv.12088 · Cited by 127 · see citing works
Kajbafzadeh, A. M., Javan-Farazmand, N., Monajemzadeh, M., & Baghayee, A. (2012) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2012.0334 · Cited by 127 · see citing works
Immunogenicity of Decellularized Porcine Liver for Bioengineered Hepatic Tissue
Mirmalek-Sani, S.-H., Sullivan, D. C., Zimmerman, C., Shupe, T. D., & Petersen, B. E. (2013) · The American Journal of Pathology · doi:10.1016/j.ajpath.2013.05.002 · Cited by 115 · see citing works
Zhou, P., Lessa, N. C., Estrada, D. K., Severson, E. B., Lingala, S., Zern, M. Α., Nolta, J. A., & Wu, J. (2011) · Liver Transplantation · doi:10.1002/lt.22270 · Cited by 108 · see citing works
Shirakigawa, N., Ijima, H., & Takei, T. (2012) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2012.05.022 · Cited by 84 · see citing works
Kock, J. D., Ceelen, L. M., Spiegelaere, W. D., Casteleyn, C. R., Claes, P., Vanhaecke, T., & Rogiers, V. (2011) · Archives of Toxicology · doi:10.1007/s00204-011-0706-1 · Cited by 72 · see citing works
Wang, X., Cui, J., Zhang, B., Zhang, H., Bi, Y., Kang, Q., Wang, N., Bie, P., Yang, Z., Wang, H., et al. (2013) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.34764 · Cited by 63 · see citing works
An efficient method for decellularization of the rat liver
Pan, M. X., Hu, P. Y., Cheng, Y., Cai, L.-Q., Rao, X. H., Wang, Y., & Gao, Y. (2013) · Journal of the Formosan Medical Association · doi:10.1016/j.jfma.2013.05.003 · Cited by 53 · see citing works
Systemic Decellularization for Multi-organ Scaffolds in Rats
Park, K.-M., & Woo, H. (2012) · Transplantation Proceedings · doi:10.1016/j.transproceed.2012.03.017 · Cited by 50 · see citing works
Preparation of immunogen-reduced and biocompatible extracellular matrices from porcine liver
Park, K.-M., Park, S.-M., Yang, S.-R., Hong, S.-H., & Woo, H.-M. (2013) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2012.08.023 · Cited by 38 · see citing works
Porcine Bioengineered Scaffolds as New Frontiers in Regenerative Medicine
Park, K.-M., & Woo, H. (2012) · Transplantation Proceedings · doi:10.1016/j.transproceed.2012.03.043 · Cited by 35 · see citing works
Liver transplantation in the high MELD era: a fair chance for everyone?
Bahra, M., & Neuhaus, P. (2011) · Langenbeck s Archives of Surgery · doi:10.1007/s00423-011-0766-y · Cited by 20 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Zhang, X., Chen, X., Hong, H., Hu, R., Liu, J., & Liu, C. (2022) · Bioactive Materials · doi:10.1016/j.bioactmat.2021.09.014 · Cited by 680 · see citing works
Decellularization for the retention of tissue niches
Moffat, D., Ye, K., & Jin, S. (2022) · Journal of Tissue Engineering · doi:10.1177/20417314221101151 · Cited by 152 · see citing works
Three-dimensional liver-derived extracellular matrix hydrogel promotes liver organoids function
Saheli, M., Sepantafar, M., Pournasr, B., Farzaneh, Z., Vosough, M., Piryaei, A., & Baharvand, H. (2018) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26622 · Cited by 148 · see citing works
Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering
McInnes, A. D., Moser, M., & Chen, D. (2022) · Journal of Functional Biomaterials · doi:10.3390/jfb13040240 · Cited by 141 · see citing works
Decellularization systems and devices: State-of-the-art
Choudhury, D., Yee, M., Sheng, Z. L. J., Amirul, A., & Naing, M. W. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.07.060 · Cited by 137 · see citing works
Current progress in hepatic tissue regeneration by tissue engineering
Hosseini, V., Maroufi, N. F., Saghati, S., Asadi, N., Darabi, M., Ahmad, S. N. S., Hosseinkhani, H., & Rahbarghazi, R. (2019) · Journal of Translational Medicine · doi:10.1186/s12967-019-02137-6 · Cited by 122 · see citing works
Elomaa, L., Keshi, E., Sauer, I. M., & Weinhart, M. (2020) · Materials Science and Engineering C · doi:10.1016/j.msec.2020.110958 · Cited by 116 · see citing works
Self-citationFunctional acellular matrix for tissue repair
Wang, B., Qinglai, T., Yang, Q., Li, M., Zeng, S., Yang, X., Xiao, Z., Tong, X., Lei, L., & Li, S. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100530 · Cited by 111 · see citing works
Verstegen, M. M. A., Willemse, J., van den Hoek, S., Kremers, G., Luider, T. M., van Huizen, N. A., Willemssen, F. E. J. A., Metselaar, H. J., IJzermans, J. N. M., van der Laan, L. J. W., et al. (2017) · Stem Cells and Development · doi:10.1089/scd.2017.0095 · Cited by 94 · see citing works
Tissue and organ decellularization in regenerative medicine
Damodaran, R. G., & Vermette, P. (2018) · Biotechnology Progress · doi:10.1002/btpr.2699 · Cited by 90 · see citing works
Massaro, M. S., Pálek, R., Rosendorf, J., Červenková, L., Liška, V., & Moulisová, V. (2021) · Materials Science and Engineering: C · doi:10.1016/j.msec.2021.112203 · Cited by 82 · see citing works
Mayorca-Guiliani, A. E., Willacy, O., Madsen, C. D., Rafaeva, M., Elisabeth Heumüller, S., Bock, F., Sengle, G., Koch, M., Imhof, T., Zaucke, F., et al. (2019) · Nature Protocols · doi:10.1038/s41596-019-0225-8 · Cited by 79 · see citing works
Napierala, H., Hillebrandt, K. H., Haep, N., Tang, P., Tintemann, M., Gassner, J. M. G. V., Nösser, M., Everwien, H., Seiffert, N., Kluge, M., et al. (2017) · Scientific Reports · doi:10.1038/srep41777 · Cited by 75 · see citing works
Self-citationStruecker, B., Hillebrandt, K. H., Voitl, R., Butter, A., Schmuck, R. B., Reutzel‐Selke, A., Geisel, D., Joehrens, K., Pickerodt, P. A., Raschzok, N., et al. (2014) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2014.0321 · Cited by 74 · see citing works
Self-citationRecent advances in liver engineering with decellularized scaffold
Dai, Q., Jiang, W., Huang, F., Song, F., Zhang, J., & Zhao, H. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.831477 · Cited by 57 · see citing works
A Hepatic Scaffold from Decellularized Liver Tissue: Food for Thought
Croce, S., Peloso, A., Zoro, T., Avanzini, M. A., & Cobianchi, L. (2019) · Biomolecules · doi:10.3390/biom9120813 · Cited by 58 · see citing works
Decellularized liver as a translucent ex vivo model for vascular embolization evaluation
Gao, Y., Li, Z., Hong, Y., Li, T., Hu, X., Sun, L., Chen, Z., Chen, Z., Luo, Z., Wang, X., et al. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.119855 · Cited by 57 · see citing works
Milan, P. B., Masoumi, F., Biazar, E., Jalise, S. Z., & Mehrabi, A. (2024) · Macromolecular Bioscience · doi:10.1002/mabi.202400322 · Cited by 41 · see citing works
Sharma, A., Rawal, P., Tripathi, D. M., Alodiya, D., Sarin, S. K., Kaur, S., & Ghosh, S. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c00671 · Cited by 37 · see citing works
Recent Advances in Decellularization and Recellularization for Tissue-Engineered Liver Grafts
Wang, Y., Nicolas, C. T., Chen, H. S., Ross, J. J., Lorenzo, S. B. D., & Nyberg, S. L. (2016) · Cells Tissues Organs · doi:10.1159/000452761 · Cited by 36 · see citing works
Biological characteristics of tissue engineered-nerve grafts enhancing peripheral nerve regeneration
Li, X., Xu, H., Li, C., Guan, Y., Liu, Y., Zhang, T., Meng, F., Cheng, H., Song, X., Jia, Z., et al. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03827-9 · Cited by 34 · see citing works
Recent Advances in Decellularization and Recellularization for Tissue-Engineered Liver Grafts
Wang, Y., Nicolas, C. T., Chen, H. S., Ross, J. J., Lorenzo, S. B. D., & Nyberg, S. L. (2017) · Cells Tissues Organs · doi:10.1159/000479597 · Cited by 33 · see citing works
Arterial Decellularized Scaffolds Produced Using an Innovative Automatic System
Pellegata, A. F., Dominioni, T., Ballo, F., Maestroni, S., Asnaghi, M. A., Zerbini, G., Zonta, S., & Mantero, S. (2014) · Cells Tissues Organs · doi:10.1159/000439082 · Cited by 32 · see citing works
da Palma, R. K., Campillo, N., Uriarte, J. J., de Oliveira, L. V. F., Navajas, D., & Farré, R. (2015) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2015.04.024 · Cited by 31 · see citing works
Hussein, K. H., Ahmadzada, B., Correa, J. C., Sultan, A., Wilken, S., Amiot, B., & Nyberg, S. L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.06.001 · Cited by 28 · see citing works
“Fortunately, the biliary network tree is confirmed to be intact after decellularization by scan electron microscopy, corrosion cast, dye perfusion, and contrast radiography; only reconstruction by bile duct cells is necessary [12,110,170,198].”
BackgroundDevelopment and Characterization of a Porcine Liver Scaffold
Ansari, T., Southgate, A., Obiri-Yeboa, I., Jones, L. G., Greco, K. V., Olayanju, A., Mbundi, L., Somasundaram, M., Davidson, B. R., & Sibbons, P. D. (2019) · Stem Cells and Development · doi:10.1089/scd.2019.0069 · Cited by 26 · see citing works
Willemse, J., van der Laan, L. J. W., de Jonge, J., & Verstegen, M. M. A. (2022) · Bioengineering · doi:10.3390/bioengineering9030110 · Cited by 26 · see citing works
Evolution of graft morphology and function after recellularization of decellularized rat livers
Butter, A., Aliyev, K., Hillebrandt, K. H., Raschzok, N., Kluge, M., Seiffert, N., Tang, P., Napierala, H., Muhamma, A. I., Reutzel‐Selke, A., et al. (2016) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2383 · Cited by 24 · see citing works
Self-citationEverwien, H., de Schellenberger, A. A., Haep, N., Tzschätzsch, H., Pratschke, J., Sauer, I. M., Braun, J., Hillebrandt, K. H., & Sack, I. (2020) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2020.103640 · Cited by 22 · see citing works
Self-citationProteomic analysis of decellularized mice liver and kidney extracellular matrices
Diedrich, A.-M., Daneshgar, A., Tang, P., Klein, O., Mohr, A., Onwuegbuchulam, O. A., von Rüden, S., Menck, K., Bleckmann, A., Juratli, M. A., et al. (2024) · Journal of Biological Engineering · doi:10.1186/s13036-024-00413-8 · Cited by 21 · see citing works
Self-citationEffect of pulsatile flow perfusion on decellularization
Park, S. M., Yang, S., Rye, S.-M., & Choi, S. W. (2018) · BioMedical Engineering OnLine · doi:10.1186/s12938-018-0445-0 · Cited by 21 · see citing works
Kasravi, M., Yaghoobi, A., Tayebi, T., Hojabri, M., Taheri, A. T., Shirzad, F., Nooshin, B. J., Mazloomnejad, R., Ahmadi, A., Tehrani, F. A., et al. (2023) · Biomaterials Advances · doi:10.1016/j.bioadv.2023.213710 · Cited by 20 · see citing works
Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device
Hillebrandt, K. H., Polenz, D., Butter, A., Tang, P., Reutzel‐Selke, A., Andreou, A., Napierala, H., Raschzok, N., Pratschke, J., Sauer, I. M., et al. (2015) · Journal of Visualized Experiments · doi:10.3791/53029 · Cited by 20 · see citing works
Self-citationElomaa, L., Gerbeth, L., Almalla, A., Fribiczer, N., Daneshgar, A., Tang, P., Hillebrandt, K. H., Seiffert, S., Sauer, I. M., Siegmund, B., et al. (2023) · Additive manufacturing · doi:10.1016/j.addma.2023.103439 · Cited by 18 · see citing works
Self-citationMußbach, F., Settmacher, U., Dirsch, O., Xie, C., & Dahmen, U. (2016) · European Surgical Research · doi:10.1159/000446211 · Cited by 18 · see citing works
Recent advances in optimization of liver decellularization procedures used for liver regeneration
Khajavi, M., Hashemi, M., & Kalalinia, F. (2021) · Life Sciences · doi:10.1016/j.lfs.2021.119801 · Cited by 14 · see citing works
A Survival Model of In Vivo Partial Liver Lobe Decellularization Towards In Vivo Liver Engineering
Wang, A., Kuriata, O., Xu, F., Nietzsche, S., Gremse, F., Dirsch, O., Settmacher, U., & Dahmen, U. (2019) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2019.0194 · Cited by 13 · see citing works
Yoshida, T., Kobayashi, M., Uomoto, S., Ohshima, K., Hara, E., Katoh, Y., Takahashi, N., Harada, T., Usui, T., Elbadawy, M., et al. (2022) · Journal of Toxicologic Pathology · doi:10.1293/tox.2022-0017 · Cited by 12 · see citing works
Bate, T. S. R., Shanahan, W. R., Casillo, J. P., Grant, R., Forbes, S. J., & Callanan, A. (2022) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.35115 · Cited by 12 · see citing works
Zhang, J.-S., Wang, Z.-B., Lai, Z.-Z., Yang, J.-W., Song, W.-J., Wei, Y.-B., Mei, J., & Wang, J.-G. (2023) · Hepatobiliary & Pancreatic Diseases International · doi:10.1016/j.hbpd.2022.10.007 · Cited by 11 · see citing works
Solid fraction determines stiffness and viscosity in decellularized pancreatic tissues
Snellings, J., Keshi, E., Tang, P., Daneshgar, A., Willma, E. C., Haderer, L., Klein, O., Krenzien, F., Malinka, T., Asbach, P., et al. (2022) · Biomaterials Advances · doi:10.1016/j.bioadv.2022.212999 · Cited by 10 · see citing works
Self-citationMir, T. A., Alzhrani, A., Nakamura, M., Iwanaga, S., Wani, S. I., Altuhami, A., Kazmi, S., Arai, K., Shamma, T., Obeid, D. A., et al. (2023) · Bioengineering · doi:10.3390/bioengineering10101126 · Cited by 9 · see citing works
Recent advances in re-engineered liver: de-cellularization and re-cellularization techniques
Wang, L., Lin, Y.-Q., Wang, J., Pan, L., Huang, K., Wan, L., Zhu, G., Liu, W., Braddock, M., & Zheng, M. (2015) · Cytotherapy · doi:10.1016/j.jcyt.2015.04.003 · Cited by 10 · see citing works
Nooshin, B. J., Tayebi, T., Babajani, A., Khani, M., & Niknejad, H. (2023) · PubMed Central · doi:10.22074/cellj.2022.557600.1081 · Cited by 9 · see citing works
Choi, S. J., Han, J., Shin, Y. H., & Kim, J. K. (2024) · Scientific Reports · doi:10.1038/s41598-024-72672-w · Cited by 9 · see citing works
Identification of tissue sections from decellularized liver scaffolds for repopulation experiments
Felgendreff, P., Schindler, C., Mußbach, F., Xie, C., Gremse, F., Settmacher, U., & Dahmen, U. (2021) · Heliyon · doi:10.1016/j.heliyon.2021.e06129 · Cited by 9 · see citing works
Whole-Liver Decellularization: Advances and Insights into Current Understanding
Fathi, I., & Eltawila, A. M. (2017) · InTech eBooks · doi:10.5772/intechopen.69487 · Cited by 7 · see citing works
A new strategy for the decellularization of whole organs by hydrostatic pressure
Morales‐Guerrero, N. A., Varela‐Echavarría, A., Lozano‐Flores, C., Vázquez‐Cuevas, F. G., Velázquez‐Miranda, E., López, J. V. R., García‐Solís, P., Solís‐S, J. C., & Hernández-Montiel, H. L. (2022) · Biotechnology Progress · doi:10.1002/btpr.3248 · Cited by 7 · see citing works
Exosome-loaded decellularized tissue: Opening a new window for regenerative medicine
Rahmati, S., Khazaei, M., Abpeikar, Z., Soleimanizadeh, A., & Rezakhani, L. (2024) · Journal of Tissue Viability · doi:10.1016/j.jtv.2024.04.005 · Cited by 8 · see citing works
Bioengineering liver tissue by repopulation of decellularised scaffolds
Afzal, Z., & Huguet, E. (2023) · World Journal of Hepatology · doi:10.4254/wjh.v15.i2.151 · Cited by 6 · see citing works
The journey of decellularized vessel: from laboratory to operating room
Kang, C., & Yang, H. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2024.1413518 · Cited by 6 · see citing works
Perfusion-Based Recellularization of Rat Livers with Islets of Langerhans
Demko, P., Hillebrandt, K. H., Napierala, H., Haep, N., Tang, P., Gassner, J. M. G. V., Kluge, M., Everwien, H., Polenz, D., Reutzel‐Selke, A., et al. (2022) · Journal of Medical and Biological Engineering · doi:10.1007/s40846-022-00697-5 · Cited by 5 · see citing works
Self-citation„Liver engineering“ als neue Quelle von Spenderorganen
Mußbach, F., Dahmen, U., Dirsch, O., & Settmacher, U. (2015) · Der Chirurg · doi:10.1007/s00104-015-0015-y · Cited by 5 · see citing works
Strategies for decellularization, re-cellularization and crosslinking in liver bioengineering
Wang, J., & Jin, X. (2024) · The International Journal of Artificial Organs · doi:10.1177/03913988231218566 · Cited by 3 · see citing works
Mirosław, P., Kosowska, K., Serafin, W., Piwowar, R., Romanik-Chruścielewska, A., Westphal, K., Wszola, M., & Klak, M. (2025) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1680938 · Cited by 3 · see citing works
The Decellularization of Whole Organs
Huang, Y. H., Yue, H., Lian, Z., & Li, X. (2021) · Springer eBooks · doi:10.1007/978-981-33-6962-7_5 · Cited by 3 · see citing works
Bonciog, D.-D., Lascu, M. R., Mâţiu-Iovan, L., Ionel, R. C., Burian, C. A., & Ordodi, V. L. (2020) · International Symposium on Electronics and Telecommunications (ISETC) · doi:10.1109/isetc50328.2020.9301149 · Cited by 3 · see citing works
Duarte-Ortega, M. F., Enríquez-Sánchez, L. B., Pérez-Ruiz, M. D., Nevárez-Rascón, A., Favila-Pérez, M. A., Castillo-González, A. R., Quiñonez-Flores, C. M., Hinojos-Gallardo, L. C., Ríos-Barrera, V. A., & Arzate-Quintana, C. (2025) · Biomimetics · doi:10.3390/biomimetics10110777 · Cited by 2 · see citing works
Liver Assist Systems for Bridging to Transplantation: Devices and Concepts
Raschzok, N., Hillebrandt, K. H., & Sauer, I. M. (2019) · Springer eBooks · doi:10.1007/978-3-319-92934-7_13 · Cited by 2 · see citing works
Self-citationTissue engineering scaffold subtypes and methods for the production thereof
Pulver, A. Y., Leibovich, B. E., Полтавцева, Р. А., Shevtsov, A. N., & Pulver, N. A. (2019) · CLINICAL AND EXPERIMENTAL MORPHOLOGY · doi:10.31088/cem2019.8.3.21-27
„Liver engineering“ als neue Quelle von Spenderorganen: Eine systematische Übersichtsarbeit
Mußbach, F., Dahmen, U., Dirsch, O., & Settmacher, U. (2016) · Der Chirurg
Li, X., Xu, H., Li, C., Guan, Y., Liu, Y., Zhang, T., Meng, F., Cheng, H., Song, X., Jia, Z., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26750480
Li, X., Xu, H., Li, C., Guan, Y., Liu, Y., Zhang, T., Meng, F., Cheng, H., Song, X., Jia, Z., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26750483
Li, X., Xu, H., Li, C., Guan, Y., Liu, Y., Zhang, T., Meng, F., Cheng, H., Song, X., Jia, Z., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26750486
- Authors
- Struecker, B., Butter, A., Hillebrandt, K., Polenz, D., Reutzel-Selke, A., Tang, P., Lippert, S., Leder, A., Rohn, S., Geisel, D., Denecke, T., Aliyev, K., Jöhrens, K., Raschzok, N., Neuhaus, P., Pratschke, J., & Sauer, I. M.
- Affiliations
- Charité – Universitätsmedizin Berlin (DE)
- General, Visceral, and Transplantation Surgery; Charité - Campus Virchow; Berlin Germany
- Corresponding author
- Sauer, Igor M.
- ORCID
- Venue
- Journal of Tissue Engineering and Regenerative Medicine
- Publisher
- Wiley
- Published
- 2014-09-04
- Volume
- 11
- Issue
- 2
- Pages
- 531-541
- ISSN
- 1932-6254 (ISSN-L)
- OpenAlex
- W1916542938
- Cited by
- 65
- References
- 37
- Citations by year
- 2025: 2 · 2024: 11 · 2023: 5 · 2022: 11 · 2021: 6 · 2020: 5 · 2019: 8 · 2018: 2 · 2017: 5 · 2016: 4 · 2015: 4 · 2014: 2
- FWCI
- 4.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Tissue Engineering and Regenerative Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- rat liver, arterial perfusion, pressure oscillations, extracellular matrix preservation, portal vein perfusion, Triton X-100, sodium dodecyl sulfate, hepatocyte growth factor, glycosaminoglycans, DNA removal, recellularization, bioartificial liver
- MeSH terms
- Animals, DNA, Extracellular Matrix, Glycosaminoglycans, Liver, Male, Oscillometry, Perfusion, Pressure, Rats, Inbred Lew, Hepatocyte Growth Factor, Tissue Engineering, Regenerative Medicine, Tissue Culture Techniques, Rats, Tissue Scaffolds
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