High acetone-butanol-ethanol production in pH-stat co-feeding of acetate and glucose
Abstract
We previously reported the metabolic analysis of butanol and acetone production from exogenous acetate by (13)C tracer experiments (Gao et al., RSC Adv., 5, 8486-8495, 2015). To clarify the influence of acetate on acetone-butanol-ethanol (ABE) production, we first performed an enzyme assay in Clostridium saccharoperbutylacetonicum N1-4. Acetate addition was found to drastically increase the activities of key enzymes involved in the acetate uptake (phosphate acetyltransferase and CoA transferase), acetone formation (acetoacetate decarboxylase), and butanol formation (butanol dehydrogenase) pathways. Subsequently, supplementation of acetate during acidogenesis and early solventogenesis resulted in a significant increase in ABE production. To establish an efficient ABE production system using acetate as a co-substrate, several shot strategies were investigated in batch culture. Batch cultures with two substrate shots without pH control produced 14.20 g/L butanol and 23.27 g/L ABE with a maximum specific butanol production rate of 0.26 g/(g h). Furthermore, pH-controlled (at pH 5.5) batch cultures with two substrate shots resulted in not only improved acetate consumption but also a further increase in ABE production. Finally, we obtained 15.13 g/L butanol and 24.37 g/L ABE at the high specific butanol production rate of 0.34 g/(g h) using pH-stat co-feeding method. Thus, in this study, we established a high ABE production system using glucose and acetate as co-substrates in a pH-stat co-feeding system with C. saccharoperbutylacetonicum N1-4.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Cancer cells can generate acetyl-CoA from cytosolic acetate.
“Cancer cells are also able to generate acetyl-CoA from cytosolic acetate (Comerford et al., 2014; Gao et al., 2016; Kamphorst et al., 2014; Mashimo et al., 2014).”
From Targeting Metabolism for Cancer Therapy (2017) · cites this paper as Gao et al., 2016
Cites
Acetone-butanol fermentation revisited
Jones, D. T., & Woods, D. (1986) · Microbiological Reviews · doi:10.1128/mr.50.4.484-524.1986 · Cited by 1,966 · see citing works
Fermentative butanol production by clostridia
Lee, S. Y., Park, J. H., Jang, S. H., Nielsen, L. K., Kim, J. H., & Jung, K. S. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.22003 · Cited by 1,033 · see citing works
Biobutanol: An attractive biofuel
Dürre, P. (2007) · Biotechnology Journal · doi:10.1002/biot.200700168 · Cited by 935 · see citing works
Ezeji, T. C., Qureshi, N., & Blaschek, H. P. (2007) · Biotechnology and Bioengineering · doi:10.1002/bit.21373 · Cited by 558 · see citing works
The use of high‐solids loadings in biomass pretreatment—a review
Modenbach, A. A., & Nokes, S. E. (2012) · Biotechnology and Bioengineering · doi:10.1002/bit.24464 · Cited by 347 · see citing works
Zverlov, V. V., Berezina, O., Velikodvorskaya, G. A., & Schwarz, W. H. (2006) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0445-z · Cited by 324 · see citing works
Ezeji, T. C., Milne, C. B., Price, N. D., & Blaschek, H. P. (2009) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-009-2390-0 · Cited by 297 · see citing works
Andersch, W., Bahl, H., & Gottschalk, G. (1983) · Applied Microbiology and Biotechnology · doi:10.1007/bf00504740 · Cited by 257 · see citing works
Tashiro, Y., Takeda, K., Kobayashi, G., et al. (2004) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(04)00279-8 · Cited by 194 · see citing works
Self-citationAcetate enhances solvent production and prevents degeneration in Clostridium beijerinckii BA101
Chen, C.-K., & Blaschek, H. (1999) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051504 · Cited by 185 · see citing works
Performance of batch, fed-batch, and continuous A–B–E fermentation with pH-control
Li, S., Srivastava, R., Suib, S. L., Li, Y., & Parnas, R. S. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.12.078 · Cited by 167 · see citing works
Coenzyme A transferase from Clostridium acetobutylicum ATCC 824 and its role in the uptake of acids
Wiesenborn, D. P., Rudolph, F. B., & Papoutsakis, E. T. (1989) · Applied and Environmental Microbiology · doi:10.1128/aem.55.2.323-329.1989 · Cited by 141 · see citing works
Kinetic modeling and sensitivity analysis of acetone–butanol–ethanol production
Shinto, H., Tashiro, Y., Yamashita, M., et al. (2007) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2007.05.005 · Cited by 134 · see citing works
Self-citationUptake and activation of acetate and butyrate in Clostridium acetobutylicum
Hartmanis, M. G. N., Klason, T., & Gatenbeck, S. (1984) · Applied Microbiology and Biotechnology · doi:10.1007/bf00254648 · Cited by 127 · see citing works
Chen, C.-K., & Blaschek, H. P. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.2.499-505.1999 · Cited by 126 · see citing works
Ishizaki, A., Michiwaki, S., Crabbe, E., et al. (1999) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(99)80044-9 · Cited by 109 · see citing works
Gottschal, J. C., & Morris, J. G. (1981) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1981.tb07679.x · Cited by 109 · see citing works
Effects of acetic and butyric acids on solvents production by Clostridium acetobutylicum
Martin, J.-R., Petitdemange, H., Ballongue, J., et al. (1983) · Biotechnology Letters · doi:10.1007/bf00132165 · Cited by 88 · see citing works
Continuous butanol fermentation from xylose with high cell density by cell recycling system
Zheng, J. S., Tashiro, Y., Yoshida, T., Gao, M., Wang, Q., & Sonomoto, K. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.11.066 · Cited by 79 · see citing works
Self-citationGu, Y., Hu, S., Chen, J., Shao, L., He, H., Yang, Y., Yang, S., & Jiang, W. (2009) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-009-0604-1 · Cited by 68 · see citing works
Oshiro, M., Hanada, K., Tashiro, Y., et al. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2673-5 · Cited by 66 · see citing works
Self-citationHiu, S. F., Zhu, C.-X., Yan, R.-T., et al. (1987) · Applied and Environmental Microbiology · doi:10.1128/aem.53.4.697-703.1987 · Cited by 60 · see citing works
Han, B., Gopalan, V., & Ezeji, T. C. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3276-5 · Cited by 56 · see citing works
mun, L. T., Ishizaki, A., Yoshino, S., et al. (1995) · Biotechnology Letters · doi:10.1007/bf00129394 · Cited by 49 · see citing works
Production of Acetic Acid by Clostridium thermoaceticum
Cheryan, M., Parekh, S. R., Shah, M. M., & Witjitra, K. (1997) · Advances in applied microbiology · doi:10.1016/s0065-2164(08)70221-1 · Cited by 47 · see citing works
Berzin, V., Kiriukhin, M. Y., & Tyurin, M. V. (2012) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2012.03272.x · Cited by 42 · see citing works
Effects of butyric and acetic acids on acetone-butanol formation by Clostridium acetobutylicum
Matta-El-Ammouri, G., Janati‐Idrissi, R., Revol‐Junelles, A., et al. (1987) · Biochimie · doi:10.1016/0300-9084(87)90242-2 · Cited by 41 · see citing works
Gao, M., Tashiro, Y., Yoshida, T., Zheng, J. S., Wang, Q., Sakai, K., & Sonomoto, K. (2014) · RSC Advances · doi:10.1039/c4ra09571e · Cited by 34 · see citing works
Self-citationNovel high butanol production from lactic acid and pentose by Clostridium saccharoperbutylacetonicum
Yoshida, T., Tashiro, Y., & Sonomoto, K. (2012) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2012.06.001 · Cited by 34 · see citing works
Self-citationRegulation of acetate kinase and butyrate kinase by acids inClostridium acetobutylicum
Ballongue, J., Amine, J., Petitdemange, H., et al. (1986) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1986.tb01546.x · Cited by 22 · see citing works
Boonsombuti, A., Komolpis, K., Luengnaruemitchai, A., & Wongkasemjit, S. (2013) · Annals of Microbiology · doi:10.1007/s13213-013-0673-2 · Cited by 17 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Targeting Metabolism for Cancer Therapy
Luengo, A., Gui, D. Y., & Vander Heiden, M. G. (2017) · Cell Chemical Biology · doi:10.1016/j.chembiol.2017.08.028 · Cited by 971 · see citing works
- Authors
- Gao, M., Tashiro, Y., Wang, Q., Sakai, K., & Sonomoto, K.
- Affiliations
- Kyushu University (JP)
- University of Science and Technology Beijing (CN)
- Corresponding author
- Sonomoto, Kenji
- Venue
- Journal of Bioscience and Bioengineering
- Publisher
- Elsevier BV
- Published
- 2016-08
- Volume
- 122
- Issue
- 2
- Pages
- 176-182
- ISSN
- 1347-4421 (ISSN-L)
- 1389-1723
- OpenAlex
- W2283043499
- Cited by
- 30
- References
- 32
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 1 · 2017: 3 · 2016: 3
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Bioscience and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- China Scholarship Council (2010646052)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Keywords
- acetone-butanol-ethanol production, Clostridium saccharoperbutylacetonicum, glucose, substrate supplementation, butanol production, acidogenesis, solventogenesis, enzyme activity, phosphate acetyltransferase, CoA transferase, acetoacetate decarboxylase
- MeSH terms
- Acetates, Acetone, Alcohol Oxidoreductases, Ethanol, Carboxy-Lyases, Clostridium, Glucose, Hydrogen-Ion Concentration, Phosphate Acetyltransferase, Coenzyme A-Transferases, Bioreactors, 1-Butanol, Batch Cell Culture Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
