CoChat
Sign Up
Cited by 97Viral Infectious Diseases and Gene Expression in InsectsMicrobial Metabolic Engineering and BioproductionMetabolomics and Mass Spectrometry Studies

Metabolomics-driven approach for the improvement of Chinese hamster ovary cell growth: overexpression of malate dehydrogenase II

Chong, William P.K., Reddy, Satty G., Yusufi, Faraaz N.K., Lee, Dong-Yup, Wong, Niki S.C., Heng, Chew Kiat, Yap, Miranda G.S., Ho, Ying Swan·2010·Journal of Biotechnology
DOI 10.1016/j.jbiotec.2010.03.018
Open this paper in CoChat — free account

Abstract

We have established a liquid chromatography-mass spectrometry based metabolomics platform to identify extracellular metabolites in the medium of recombinant Chinese hamster ovary (CHO) fed-batch reactor cultures. Amongst the extracellular metabolites identified, malate accumulation was the most significant. The contributing factors to malate efflux were found to be the supply of aspartate from the medium, and an enzymatic bottleneck at malate dehydrogenase II (MDH II) in the tricarboxylic acid cycle. Subsequent metabolic engineering to overexpress MDH II in CHO resulted in increases in intracellular ATP and NADH, and up to 1.9-fold improvement in integral viable cell number.

Integrity signals

Registry

Crossref · 10.1016/j.jbiotec.2010.03.018

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • A bottleneck at the malate dehydrogenase II (MDHII) level was characterized for the tricarboxylic acid (TCA) cycle in CHO cells and pyruvate metabolism was shown to vary between high producing and low producing anti-CD20 CHO clones.

    “A bottleneck at the malate dehydrogenase II (MDHII) level was characterized for the tricarboxylic acid (TCA) cycle in CHO cells and pyruvate metabolism was shown to vary between high producing and low producing anti-CD20 CHO clones (Chong et al., 2010;Ghorbaniaghdam et al., 2014).”

    From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Chong et al., 2010

Cited by

Sorted by citations

Authors
Chong, W. P. K., Reddy, S. G., Yusufi, F. N. K., Lee, D.-Y., Wong, N. S. C., Heng, C. K., Yap, M. G. S., & Ho, Y. S.
Affiliations
  • Agency for Science, Technology and Research (SG)
  • National University of Singapore (SG)
Corresponding author
Chong, William P.K.
ORCID
Venue
Journal of Biotechnology
Publisher
Elsevier BV
Published
2010-05-17
Volume
147
Issue
2
Pages
116-121
ISSN
0168-1656 (ISSN-L)
DOI
10.1016/j.jbiotec.2010.03.018
OpenAlex
W2092015535
Cited by
97
References
0
Citations by year
2026: 4 · 2025: 6 · 2024: 5 · 2023: 4 · 2022: 1 · 2021: 5 · 2020: 5 · 2019: 5 · 2018: 6 · 2017: 7 · 2016: 4 · 2015: 10 · 2014: 9 · 2013: 9 · 2012: 9
FWCI
3.38
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
  • National University of Singapore (submittedVersion)
Funders
Biomedical Research Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords
CHO cells, metabolic engineering, extracellular metabolites, malate accumulation, TCA cycle, fed-batch fermentation
MeSH terms
Animals, Aspartic Acid, Cell Count, Chromatography, Liquid, Cricetulus, Cricetinae, Malate Dehydrogenase, Malates, Mass Spectrometry, CHO Cells, Cell Culture Techniques, Cell Growth Processes, Metabolic Networks and Pathways, Metabolomics

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review