Simultaneous determination of Vitamin A, 25-hydroxyl vitamin D 3 α-tocopherol in small biological fluids by liquid chromatography-tandem mass spectrometry
Abstract
A sensitive method for the simultaneous analysis of vitamin A (VA), 25-hydroxyl vitamin D 3 (25-OH VD 3 ) and α-tocopherol (VE) in children plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated. Sample preparation chose the solid phase extraction. 100 μL of plasma was mixed with 300 μL ethanol contained 4 μL isotope-labelled analytes. After a series operation, the supernatant was applied to the solid phase extraction (SPE) plate (HLB μElution plate). The eluate was evaporated, and reconstituted in 100 μL methanol. And then, 6 μL reconstituted sample was injected into LC-MS/MS. Quantitative analysis was carried out by multiple reaction monitoring (MRM) with a positive mode electrospray (ESi + ). Separations of VA, 25-OH VD 3 and VE were performed on an Acquity UPLC reversed-phase Phenyl-Hexyl analytical column (CSH, 2.1 × 50 mm, 1.7 μm). Gradient elution was used at a flow rate of 0.4 mL/min with a mobile phase consisting of 0.1% formic acid solution and 0.1% formic acid, 5 mM ammonium formate in acetonitrile. The total time of analysis was 10 min. The method had a limit of quantification (LOQ) of 10.03, 1.20, and 0.04 ng/mL for VA, 25-OH VD 3 and VE in methanol, respectively. The linear calibration curves were fitted over the range of 0.14-14.32 μg/mL, 1.80-180.29 ng/mL, and 6.03-602.99 ng/mL for VA, 25-OH VD 3 and VE in methanol. The correlation coefficients were greater than 0.998 for all analytes. The recoveries for all analyt…
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MS/MS is currently the most sensitive method used for making bioanalyses of retinoic acid and is readily coupled with LC separations capable of resolving isomers of retinoic acid.
“MS/MS is currently the most sensitive method used for making bioanalyses of retinoic acid and is readily coupled with LC separations capable of resolving isomers of retinoic acid.”
From Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity (2021) · cites this paper as [298]
MS/MS offers appropriate sensitivity for the detection through background reductions of 100-1000-fold over MS.
“MS/MS offers appropriate sensitivity for the detection through background reductions of 100–1000-fold over MS.”
From Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity (2021) · cites this paper as [298]
GC-MS allows for good sensitivity but requires additional derivatization steps for making retinoid acid analyses, often representing labor-intensive procedures and a potential source of errors.
“GC–MS allows for good sensitivity but requires additional derivatization steps for making retinoid acid analyses, often representing labor-intensive procedures and a potential source of errors.”
From Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity (2021) · cites this paper as [298]
Triple-quadrupole LC-MS/MS facilitates the most effective qualitative-quantitative analysis of retinoic acid with sufficient sensitivity and specificity, yet no requirement for derivatization or definite mass identification.
“Triple-quadrupole LC–MS/MS facilitates the most effective qualitative–quantitative analysis of retinoic acid with sufficient sensitivity and specificity, yet no requirement for derivatization or definite mass identification.”
From Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity (2021) · cites this paper as [298]
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Peersman, N., Elslande, J. V., Lepage, Y., De Amicis, S., Desmet, K., & Vermeersch, P. (2020) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2019-1237 · Cited by 12 · see citing works
- Authors
- Zhang, H., Quan, L., Pei, P., Lin, Y., Feng, C., Guan, H., Wang, F., Zhang, T., Wu, J., & Huo, J.
- Affiliations
- Capital Institute of Pediatrics (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Chinese Center For Disease Control and Prevention (CN)
- Corresponding author
- Wu, Jianxin
- Venue
- Journal of Chromatography B
- Publisher
- Elsevier BV
- Published
- 2018-03
- Volume
- 1079
- Pages
- 1-8
- ISSN
- 1570-0232 (ISSN-L)
- OpenAlex
- W2773462249
- Cited by
- 29
- References
- 2
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 4 · 2021: 4 · 2020: 8 · 2018: 2
- FWCI
- 1.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography B (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81501838)
- Beijing Talents Fund (2014000021469G229)
- Contract Grant Sponsors: Chinese National “973” Program on Population and Health (2013CB945404)
- CAMS Initiative for Innovative Medicine (2016-I2M-1-008)
- Research of Beijing Medical (2016-4)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- vitamin A, 25-hydroxyvitamin D3, alpha-tocopherol, vitamin E, plasma, children, simultaneous determination, solid-phase extraction, isotope-labeled internal standards, vitamin status
- MeSH terms
- Calibration, Child, Cholecalciferol, Chromatography, High Pressure Liquid, Humans, Vitamin A, alpha-Tocopherol, Solid Phase Extraction, Tandem Mass Spectrometry, Limit of Detection
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