Cationic charge‐dependent hepatic delivery of amidated serum albumin
Abstract
To obtain a quantitative correlation between the physicochemical properties of amidated bovine serum albumin (BSA) and their tissue distribution characteristics for the development of targeted delivery of proteins, BSA was amidated with hexamethylenediamine (HMD) or ethylenediamine (ED) to obtain cationized BSAs. Their structural changes were examined by spectroscopic and electrophoretic techniques then their tissue distribution was studied in mice. Circular dichroism (CD) and fluorescence measurements showed that spectroscopic changes occurred as the number of free NH2 groups increased. Capillary electrophoresis revealed a linear relationship between the mobility and the increased number of free NH2 groups. 111In-cationized BSAs were rapidly taken up by liver, but HMD-BSA showed a faster uptake than ED-BSA with a similar number of free NH2 groups, suggesting that the diamine reagent with a longer carboxyl side chain results in more efficient hepatic targeting. The hepatic uptake clearance (CL(liver)) of both derivatives increased significantly with a decrease in electrophoretic mobility (mu(ep)) towards the anode and reached a plateau at low electrophoretic mobility. The electrophoretic mobility is an appropriate indicator of the degree of amidation, which was closely correlated with the hepatic uptake clearance. The correlation between the mobility and the clearance shows that a low degree of amidation is sufficient for efficient hepatic targeting of proteins.
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The breakdown of serum albumin is complicated by the fact that after oxidation, glycation, or binding of pro-oxidative substances, serum albumin loses its antioxidant capacity and is more rapidly degraded than reduced unbound serum albumin.
“The breakdown of serum albumin is complicated by the fact that after oxidation, glycation, or binding of pro‐oxidative substances, serum albumin loses its antioxidant capacity and is more rapidly degraded than reduced unbound serum albumin.79, 80, 81, 82, 83”
From Hypoalbuminemia: Pathogenesis and Clinical Significance (2019) · cites this paper as [80]
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- Authors
- Ma, S.-F., Nishikawa, M., Katsumi, H., Yamashita, F., & Hashida, M.
- Affiliations
- Kyoto University (JP)
- Corresponding author
- Hashida, Mitsuru
- Venue
- Journal of Controlled Release
- Publisher
- Elsevier BV
- Published
- 2005-02
- Volume
- 102
- Issue
- 3
- Pages
- 583-594
- ISSN
- 0168-3659 (ISSN-L)
- OpenAlex
- W2036485362
- Cited by
- 66
- References
- 33
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 4 · 2021: 1 · 2020: 5 · 2019: 6 · 2018: 7 · 2017: 7 · 2016: 1 · 2015: 4 · 2014: 3 · 2013: 2 · 2012: 3
- FWCI
- 1.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Controlled Release (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- hepatic targeting, protein delivery, hexamethylenediamine, ethylenediamine, electrophoretic mobility, tissue distribution
- MeSH terms
- Amides, Animals, Cations, Electrophoresis, Liver, Male, Serum Albumin, Drug Delivery Systems, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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