Cited by 382Nanoparticle-Based Drug DeliveryImmunotherapy and Immune ResponsesRNA Interference and Gene Delivery

The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.

PEGylated liposomes elicit an anti-PEG IgM response in a T cell-independent manner

Ishida, Tatsuhiro, Wang, XinYu, Shimizu, Taro, Nawata, Kosuke, Kiwada, Hiroshi·2007·Journal of Controlled Release

DOI 10.1016/j.jconrel.2007.05.015

Open this paper in CoChat — free account

Abstract

We recently reported that intravenous injections of "empty" PEGylated liposomes without encapsulated or surface coupled proteins elicit a PEG-specific IgM response in rats. In the present study, simultaneous weak anti-PEG IgG and strong anti-PEG IgM responses were detected following intravenous injections of "empty" PEGylated liposomes. The pattern of immune response appears to differ from the classic primary response against T cell-dependent (TD) antigens. The anti-PEG IgM response was detected in T-cell deficient nude BALB/c mice following intravenous injection of "empty" PEGylated liposomes, suggesting that "empty" PEGylated liposomes initiate the immune response against PEG in a T cell-independent manner. In vitro splenic lymphocytes-proliferation assay indicated that TNP-LPS, a typical type 1 T cell-independent (TI) antigen (TI-1 antigen), significantly primed the proliferation, while TNP-Ficoll, a typical type 2 TI antigen (TI-2 antigen), and "empty" PEGylated liposomes did not prime any proliferation under these experimental conditions. In addition, in splenic marginal zone (MZ) B-cell-depleted rats, the anti-PEG IgM response was diminished, while the immune reactions against TNP-BSA (a TD antigen) and TNP-LPS (TI-1 antigen) were not diminished. These results demonstrate that "empty" PEGylated liposomes may promote the immune response against PEG as a result of priming the activation of MZ B cells, as TI-2 antigen promotes a specific IgM response. In conclusion, although the mechanistic details behind the immune reaction against "empty" PEGylated liposomes are not yet clear, the liposomes elicit an anti-PEG IgM response in a T cell-independent manner and appear to be a TI-2 antigen, and splenic MZ B cells may be essential for the immune response against "empty" PEGylated liposomes.

Integrity signals

Registry

Crossref · 10.1016/j.jconrel.2007.05.015

Cites

45 of 45 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes
Klibanov, A. L., Maruyama, K., Torchilin, V. P., & Huang, L. (1990) · FEBS Letters · doi:10.1016/0014-5793(90)81016-h · Cited by 2,030
Sterically stabilized liposomes: improvements in pharmacokinetics and antitumor therapeutic efficacy.
Papahadjopoulos, D., Allen, T. M., Gabizón, A., Mayhew, E. G., Matthay, K. K., Huang, S. K., Lee, K. D., Woodle, M. C., Lasiç, D. D., & Redemann, C. T. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.24.11460 · Cited by 1,621
Liposomes containing synthetic lipid derivatives of poly(ethylene glycol) show prolonged circulation half-lives in vivo
Allen, T. M., Hansen, C., Martin, F., Redemann, C. T., & Yau‐Young, A. O. (1991) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(91)90246-5 · Cited by 1,392
Marginal Zone and B1 B Cells Unite in the Early Response against T-Independent Blood-Borne Particulate Antigens
Martin, F., Oliver, A. M., & Kearney, J. F. (2001) · Immunity · doi:10.1016/s1074-7613(01)00129-7 · Cited by 987
T Cell-Independent Antigens Type 2
Mond, J. J., Lees, A., & Snapper, C. M. (1995) · Annual Review of Immunology · doi:10.1146/annurev.iy.13.040195.003255 · Cited by 833
Accelerated Blood Clearance and Altered Biodistribution of Repeated Injections of Sterically Stabilized Liposomes
Dams, E. T. M., Laverman, P., Oyen, W. J. G., Storm, G., Scherphof, G. L., van der Meer, J. W. M., Corstens, F. H. M., & Boerman, O. C. (2000) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)35391-1 · Cited by 606
Injection of PEGylated liposomes in rats elicits PEG-specific IgM, which is responsible for rapid elimination of a second dose of PEGylated liposomes
Ishida, T., Ichihara, M., Wang, X., Yamamoto, K., Kimura, J., Majima, E., & Kiwada, H. (2006) · Journal of Controlled Release · doi:10.1016/j.jconrel.2006.01.005 · Cited by 558
Self-citation
Sterically stabilized liposomes: a hypothesis on the molecular origin of the extended circulation times
Lasiç, D. D., Martin, F. J., Gabizón, A., Huang, S. K., & Papahadjopoulos, D. (1991) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(91)90162-2 · Cited by 545
Influence of surface hydrophilicity of liposomes on their interaction with plasma protein and clearance from the circulation: Studies with poly(ethylene glycol)-coated vesicles
Senior, J. H., Delgado, C., Fisher, D., Tilcock, C., & Gregoriadis, G. (1991) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(91)90337-8 · Cited by 521
Poly(ethylene glycol) on the liposome surface: on the mechanism of polymer-coated liposome longevity
Torchilin, V. P., Omelyanenko, V. G., Papisov, M. I., Bogdanov, A. A., Jr., Trubetskoy, V. S., Herron, J. N., & Gentry, C. A. (1994) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(94)90003-5 · Cited by 466
Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses
Oliver, A. M., Martin, F., Gartland, G. L., Carter, R. H., & Kearney, J. F. (1997) · European Journal of Immunology · doi:10.1002/eji.1830270935 · Cited by 427
Anti-PEG IgM elicited by injection of liposomes is involved in the enhanced blood clearance of a subsequent dose of PEGylated liposomes
Wang, X., Ishida, T., & Kiwada, H. (2007) · Journal of Controlled Release · doi:10.1016/j.jconrel.2007.02.010 · Cited by 421
Self-citation
T-Independent Immune Response: New Aspects of B Cell Biology
Fagarasan, S., & Honjo, T. (2000) · Science · doi:10.1126/science.290.5489.89 · Cited by 415
Colorimetric Assay Methods for Free and Phosphorylated Glyceric Acids
Bartlett, G. R. (1959) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)70227-5 · Cited by 412
Accelerated blood clearance of PEGylated liposomes following preceding liposome injection: Effects of lipid dose and PEG surface-density and chain length of the first-dose liposomes
Ishida, T., Harada, M., Wang, X., Ichihara, M., Irimura, K., & Kiwada, H. (2005) · Journal of Controlled Release · doi:10.1016/j.jconrel.2005.04.003 · Cited by 365
Self-citation
Positive Selection from Newly Formed to Marginal Zone B Cells Depends on the Rate of Clonal Production, CD19, and btk
Martin, F., & Kearney, J. F. (2000) · Immunity · doi:10.1016/s1074-7613(00)80157-0 · Cited by 351
Accelerated clearance of PEGylated liposomes in rats after repeated injections
Ishida, T., Maeda, R., Ichihara, M., Irimura, K., & Kiwada, H. (2003) · Journal of Controlled Release · doi:10.1016/s0168-3659(02)00462-5 · Cited by 327
Self-citation
Factors Affecting the Accelerated Blood Clearance of Polyethylene Glycol-Liposomes upon Repeated Injection
Laverman, P., Carstens, M. G., Boerman, O. C., Dams, E. T. M., Oyen, W. J. G., van Rooijen, N., Corstens, F. H. M., & Storm, G. (2001) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)29419-2 · Cited by 298
Accelerated blood clearance of PEGylated liposomes upon repeated injections: Effect of doxorubicin-encapsulation and high-dose first injection
Ishida, T., Atobe, K., Wang, X., & Kiwada, H. (2006) · Journal of Controlled Release · doi:10.1016/j.jconrel.2006.08.017 · Cited by 290
Self-citation
T cell independent antigens
Mond, J. J. (1995) · Current Opinion in Immunology · doi:10.1016/0952-7915(95)80109-x · Cited by 285
Spleen plays an important role in the induction of accelerated blood clearance of PEGylated liposomes
Ishida, T., Ichihara, M., Wang, X., & Kiwada, H. (2006) · Journal of Controlled Release · doi:10.1016/j.jconrel.2006.08.001 · Cited by 270
Self-citation
The dual function of the splenic marginal zone: essential for initiation of anti-TI-2 responses but also vital in the general first-line defense against blood-borne antigens
Zandvoort, A., & Timens, W. (2002) · Clinical & Experimental Immunology · doi:10.1046/j.1365-2249.2002.01953.x · Cited by 225
Thymus-independent antigens: complexity of B-lymphocyte activation revealed
Mosier, D. E., & Subbarao, B. (1982) · Immunology Today · doi:10.1016/0167-5699(82)90095-0 · Cited by 220
Immunogenicity and Rapid Blood Clearance of Liposomes Containing Polyethylene Glycol-Lipid Conjugates and Nucleic Acid
Semple, S. C., Harasym, T. O., Clow, K. A., Ansell, S. M., Klimuk, S. K., & Hope, M. J. (2005) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.104.078113 · Cited by 211
Accelerated clearance of a second injection of PEGylated liposomes in mice
Ishida, T., Masuda, K., Ichikawa, T., Ichihara, M., Irimura, K., & Kiwada, H. (2003) · International Journal of Pharmaceutics · doi:10.1016/s0378-5173(03)00085-1 · Cited by 185
Self-citation
Splenic dependence of the antibody response to thymus‐independent (TI‐2) antigens
Amlot, P. L., Grennan, D., & Humphrey, J. (1985) · European Journal of Immunology · doi:10.1002/eji.1830150516 · Cited by 145
Staphylococcal toxin induced preferential and prolonged in vivo deletion of innate-like B lymphocytes
Goodyear, C. S., & Silverman, G. J. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0404382101 · Cited by 108
Role of T lymphocytes in the response to TNP-AECM-Ficoll.
Mond, J. J., Mongini, P. K. A., Sieckmann, D. G., & Paul, W. E. (1980) · The Journal of Immunology · doi:10.4049/jimmunol.125.3.1066 · Cited by 105
Systemic administration of LPD prepared with CpG oligonucleotides inhibits the growth of established pulmonary metastases by stimulating innate and acquired antitumor immune responses
Whitmore, M., Li, S., Falo, L. D., & Huang, L. (2001) · Cancer Immunology Immunotherapy · doi:10.1007/s002620100227 · Cited by 101
Immune Stimulation by a CpG-Containing Oligodeoxynucleotide Is Enhanced When Encapsulated and Delivered in Lipid Particles
Mui, B. L., Raney, S. G., Semple, S. C., & Hope, M. J. (2001) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)29491-x · Cited by 90
Immunological functions and in vivo cell-cell interactions of T cells in the spleen.
van den Eertwegh, A. J. M., Boersma, W. J. A., & Claassen, E. A. W. (1992) · PubMed · Cited by 66
Spleen Capture of Nanoparticles: Influence of Animal Species and Surface Characteristics
Demoy, M., Andreux, J. P., Weingarten, C., Gouritin, B., Guilloux, V., & Couvreur, P. (1999) · Pharmaceutical Research · doi:10.1023/a:1018858409737 · Cited by 63
T cell dependence and factor reconstitution of in vitro antibody responses to TNP-B. Abortus and TNP-Ficoll: restoration of depleted responses with chromatographed fractions of a T cell-derived factor.
Mond, J. J., Farrar, J., Paul, W. E., Fuller-Farrar, J., Schaefer, M. L., & Howard, M. (1983) · The Journal of Immunology · doi:10.4049/jimmunol.131.2.633 · Cited by 59
Enhanced immune response to T-independent antigen by using CpG oligodeoxynucleotides encapsulated in liposomes
Li, W. M., Bally, M. B., & Schutze-Redelmeier, M.-P. M. (2001) · Vaccine · doi:10.1016/s0264-410x(01)00277-8 · Cited by 53
Immunogenic Properties of Liposomal Model Membranes in Mice
Yasuda, T., Dancey, G. F., & Kinsky, S. C. (1977) · The Journal of Immunology · doi:10.4049/jimmunol.119.6.1863 · Cited by 50
Characterization of immunogenic properties of haptenated liposomal model membranes in mice. I. Thymus independence of the antigen.
van Houte, A. J., Snippe, H., & Willers, J. M. (1979) · PubMed · Cited by 41
Lymphoid bone marrow cultures can reconstitute heterogeneous B and T cell-dependent responses in severe combined immunodeficient mice.
Dorshkind, K. A., Denis, K. A., & Witte, O. N. (1986) · The Journal of Immunology · doi:10.4049/jimmunol.137.11.3457 · Cited by 41
Interaction of anti-cholesterol antibodies with human lipoproteins
Dijkstra, J., Swartz, G. M., Raney, J. J., Aniagolu, J. U., Toro, L. A., Nacy, C. A., & Green, S. J. (1996) · The Journal of Immunology · doi:10.4049/jimmunol.157.5.2006 · Cited by 40
Staphylococcal Protein A Deletes B-1a and Marginal Zone B Lymphocytes Expressing Human Immunoglobulins: An Immune Evasion Mechanism
Viau, M., Longo, N. S., Lipsky, P. E., & Zouali, M. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.11.7719 · Cited by 34
Lipopolysaccharide from Brucella abortus behaves as a T-cell-independent type 1 carrier in murine antigen-specific antibody responses
Betts, M. R., Beining, P. R., Brunswick, M., Inman, J. K., Angus, R. D., Hoffman, T., & Golding, B. (1993) · Infection and Immunity · doi:10.1128/iai.61.5.1722-1729.1993 · Cited by 31
Characterization of immunogenic properties of haptenated liposomal model membranes in mice.
Aj, V. H., Snippe, H., Peulen, G. T., & Willers, J. M. N. (1981) · PubMed · Cited by 26
Selective loss of large lymphocytes from the marginal zone of the white pulp in rat spleens following a single dose of cyclophosphamide. A study using quantitative histological methods.
Kumararatne, D. S., Gagnon, R. F., & Smart, Y. C. (1980) · PubMed · Cited by 23
Immunoregulation in the rat: requirements for in vitro B cell responses to classical TI-1 and TI-2 antigens.
Morisaki, I., Eldridge, J. H., Michalek, S. M., Hamada, S., & McGhee, J. R. (1983) · The Journal of Immunology · doi:10.4049/jimmunol.131.3.1131 · Cited by 11
Humoral and cellular immune responses in gluten-treated suckling or hand-fed rats.
Kozáková, H., Štëpánková, R., Tučková, L., Šinkora, M., Jelinkova, L. P., & Tlaskalová-Hogenová, H. (2000) · Physiological Research · doi:10.33549/physiolres.930000.49.665 · Cited by 7
Theoretical basis for development of liposomes as carriers of vaccines
Alving, C. R. (1998) · Elsevier eBooks · doi:10.1016/b978-044482917-7/50009-0 · Cited by 5

Research works printed but not linked: 1

Li, 2001, Enhanced immune response to T-independent antigen by using CpG oligodeoxynucleotides encapsulated in liposomes, Vaccine, 20, 148, 10.1016/S0264-410X(01)00277-8 · DOI

Sorted by citations

PEGylation as a strategy for improving nanoparticle-based drug and gene delivery
Suk, J. S., Xu, Q., Kim, N., Hanes, J., & Ensign, L. M. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.09.012 · Cited by 4,523
Challenges in Development of Nanoparticle-Based Therapeutics
Desai, N. (2012) · The AAPS Journal · doi:10.1208/s12248-012-9339-4 · Cited by 1,173
Minireview: Nanoparticles and the Immune System
Zolnik, B. S., González-Fernández, A., Sadrieh, N., & Dobrovolskaia, M. A. (2010) · Endocrinology · doi:10.1210/en.2009-1082 · Cited by 914
SERS Nanoparticles in Medicine: From Label-Free Detection to Spectroscopic Tagging
Lane, L. A., Qian, X., & Nie, S. (2015) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00265 · Cited by 881
Curcumin nanoformulations: A review of pharmaceutical properties and preclinical studies and clinical data related to cancer treatment
Naksuriya, O., Okonogi, S., Schiffelers, R. M., & Hennink, W. E. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.12.090 · Cited by 856

See all 382

Authors: Ishida, T., Wang, X., Shimizu, T., Nawata, K., & Kiwada, H.
Affiliations: Tokushima University (JP)
Corresponding author: Kiwada, Hiroshi
ORCID: Ishida, Tatsuhiro
Shimizu, Taro
Venue: Journal of Controlled Release
Publisher: Elsevier BV
Published: 2007-10
Volume: 122
Issue: 3
Pages: 349-355
ISSN: 0168-3659 (ISSN-L)
DOI: 10.1016/j.jconrel.2007.05.015
OpenAlex: W2087167636
Cited by: 382
References: 45
Citations by year: 2026: 13 · 2025: 20 · 2024: 14 · 2023: 28 · 2022: 20 · 2021: 23 · 2020: 25 · 2019: 13 · 2018: 32 · 2017: 22 · 2016: 24 · 2015: 29 · 2014: 24 · 2013: 29 · 2012: 25
FWCI: 4.91
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Journal of Controlled Release (publishedVersion)
PubMed (publishedVersion)
Funders: Kao Foundation for Arts and Sciences
Topics: Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords: PEGylated liposomes, T cell-independent immunity, marginal zone B cells, nude mice, TNP-LPS, TNP-Ficoll, splenocyte proliferation, intravenous injection
MeSH terms: Animals, Antigens, T-Independent, Cyclophosphamide, Immunoglobulin G, Immunoglobulin M, Injections, Intravenous, Liposomes, Male, Mice, Inbred BALB C, Mice, Nude, Polyethylene Glycols, Spleen, T-Lymphocytes, Rats, Wistar, Cell Proliferation, Mice, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

PEGylated liposomes elicit an anti-PEG IgM response in a T cell-independent manner

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks