The minimal enzyme cocktail concept for biomass processing
Abstract
Efficient generation of a fermentable hydrolysate is a primary necessity in the utilization of fibrous plant biomass as a feedstock in bioethanol processes. Enzyme catalyzed hydrolysis of cellulose and heteroxylans in biomass feedstocks each require multiple enzyme activities to achieve degradation to fermentable monosaccharides. The minimal enzyme cocktail concept concerns identification of the minimal number, the minimal levels, and the optimal combination of the best performing key monoactive enzymatic activities to meet this requirement. Two major hypotheses lie behind this concept: 1. That the native multi-component profiles of crude “wild type” cellulolytic and/or xylan degrading enzyme preparations are not optimal for degradation of cellulose in pre-treated lignocellulosic biomass nor for degradation of heteroxylans in hemicellulose-rich product streams; 2. That it is possible to replace crude multienzyme preparations with designed combinations of the minimal number of required enzyme activities for biomass processing. This paper outlines the current strategies employed and the stage of development of minimal enzyme cocktails for enzymatic hydrolysis of cellulose and arabino-xylan in complex, genuine biomass substrates. The available data demonstrate the feasibility of the concept and illustrate the potential efficacy improvements obtainable by use of designed minimal enzyme cocktails for pre-treated lignocellulosic and hemicellulose-rich biomass substrates.
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Only various crude, commercial enzyme mixtures designed for plant material processing have been used as hydrolysis aids for extraction of brown seaweed biofunctional polysaccharide fractions, compared with the advanced stage of designed enzyme mixtures for complex plant materials or heterogeneous plant cell wall polysaccharides.
“However, as compared to the quite advanced stage of development of designed enzyme mixtures for hydrolysis of complex plant materials or heterogenous plant cell wall polysaccharides, 39,40 only various crude, commercial enzyme mixtures, designed for plant material processing, have been used as hydrolysis aids for extraction of brown seaweed biofunctional polysaccharide fractions.5,41”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [40]
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- Authors
- Meyer, A. S., Rosgaard, L., & Sørensen, H. R.
- Affiliations
- Technical University of Denmark (DK)
- University of Copenhagen (DK)
- Corresponding author
- Meyer, Anne S.
- ORCID
- Meyer, Anne S.
- Venue
- Journal of Cereal Science
- Publisher
- Elsevier BV
- Published
- 2009-11
- Volume
- 50
- Issue
- 3
- Pages
- 337-344
- ISSN
- 0733-5210 (ISSN-L)
- OpenAlex
- W1984595980
- Cited by
- 115
- References
- 49
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 6 · 2022: 3 · 2021: 7 · 2020: 5 · 2019: 3 · 2018: 9 · 2017: 6 · 2016: 10 · 2015: 10 · 2014: 14 · 2013: 7 · 2012: 6
- FWCI
- 5.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cereal Science (publishedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- enzymatic hydrolysis, cellulose degradation, xylan degradation, lignocellulosic biomass, bioethanol feedstock, cellulolytic enzymes
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