Evaluation of a novel real-time RT-PCR using TOCE technology compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses
Open accessAbstract
Background Various kinds of commercial molecular systems have been developed for fast and more accurate detection of respiratory viruses. Anyplex™ II RV16 [RV16] was designed for simultaneous detection of 16 respiratory viruses using multiplex PCR coupled with TOCE™ technology. Objectives To compare the performance of RV16 with those of culture and Seeplex(®) RV15 ACE [RV15] by determining their sensitivity and specificity. Study design Seven hundred and thirty respiratory samples were tested by modified shell vial culture method, RV16, and RV15. For molecular tests, automated nucleic acid extraction and liquid handling system using MICROLAB Nimbus IVD (Hamilton, USA) was adopted to maximize the workflow and accuracy. Performance of each assay was determined against a composite reference standard. Results Two hundred and one samples (28%) out of 730 samples were positive by culture, while additional 281 (39%) were positive by RV16 or RV15. Sensitivities of RV16, RV15, and culture for virus tested were as follows: 100/93/63% for influenza A, 90/80/69% for influenza B, 98/94/63% for RSV, 98/52/23% for adenovirus, and 100/75/46% for PIV. For viruses not covered by culture, sensitivities of RV16 and RV15 were as follows: 99/81% for rhinovirus, 92/100% for coronavirus OC43, 100/56% for coronavirus 229E/NL63, 92/88% for metapneumovirus, 100/62% for bocavirus, and 91/91% for enterovirus. Overall, the specificities of culture, RV16, and RV15 (Seegene) were 100/99.9/99.9%. Conclusions RV16 assay was superior to culture method and RV15 and will be a promising tool for patient management and public health epidemiology.
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These HPV51 subtypes were likely to be suboptimally detected by H28, whose sensitivity relies on type-specific primers and probes strictly adapted to published sequences.
“They therefore were likely to be suboptimally detected by H28, whose sensitivity relies on type-specific primers and probes strictly adapted to published sequences (25).”
From Comparison of Seegene Anyplex II HPV28 with the PGMY-CHUV Assay for Human Papillomavirus Genotyping (2014) · cites this paper as [25]
The Discussion considers possible probe–target mismatches in influenza B assays, not detection of HPV51 subtypes by H28. The Analytical methods section describes respiratory-virus assays. Searches for HPV51, H28, and the claimed type-specific primers and probes found no passage addressing the claim. (LLM, conf=0.78)
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Comparison of Seegene Anyplex II HPV28 with the PGMY-CHUV Assay for Human Papillomavirus Genotyping
Estrade, C., & Sahli, R. (2014) · Journal of Clinical Microbiology · doi:10.1128/jcm.02749-13 · Cited by 57 · see citing works
- Authors
- Cho, C. H., Chulten, B., Lee, C. K., Nam, M. H., Yoon, S. Y., Lim, C. S., Cho, Y., & Kim, Y. K.
- Affiliations
- Korea University (KR)
- Corresponding author
- Lee, Chang Kyu
- Venue
- Journal of Clinical Virology
- Publisher
- Elsevier BV
- Published
- 2013-08
- Volume
- 57
- Issue
- 4
- Pages
- 338-342
- ISSN
- 1386-6532 (ISSN-L)
- PMC
- PMC7108272
- OpenAlex
- W2167345993
- Cited by
- 49
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 6 · 2018: 6 · 2017: 3 · 2016: 8 · 2015: 9 · 2014: 4 · 2013: 4
- FWCI
- 6.08
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Clinical Virology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Korea University
- Korea University Anam Hospital
- Topics
- Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Keywords
- respiratory viruses, multiplex RT-PCR, viral culture, influenza A virus, influenza B, respiratory syncytial virus, adenovirus, parainfluenza virus, rhinovirus, HCoV-OC43, HCoV-229E, coronavirus NL63, metapneumovirus, bocavirus, enterovirus, sensitivity and specificity
- MeSH terms
- Cells, Cultured, Humans, Respiratory Tract Infections, Sensitivity and Specificity, Viruses, Reverse Transcriptase Polymerase Chain Reaction, Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction
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