Cited by 68Medicinal Plant Pharmacodynamics ResearchBone Metabolism and DiseasesBone health and osteoporosis research

Osteoprotective effect of extract from Achyranthes bidentata in ovariectomized rats

He, Cui-Cui, Hui, Rong-Rong, Tezuka, Yasuhiro, Kadota, Shigetoshi, Li, Jian-Xin·2010·Journal of Ethnopharmacology

Abstract

Aim of the study A Chinese herbal drug, root of Achyranthes bidentata showed a potent inhibitory activity on bone resorption induced by parathyroid hormone (PTH) in a bone organ culture using neonatal mouse parietal bones. The present study is to clarify the fractions responsible for the activity and further explore the osteoprotective effect of the fraction in vivo.Materials and methods The hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH) and water soluble fractions of methanol extract of the root of Achyranthes bidentata were prepared and screened for their anti-bone resorption activity using the bone organ culture system. The n-BuOH soluble fraction was further administered orally at doses of 25, 50 and 100mg/(kgday) to ovariectomized (OVX) rats. The analyses of the rat body weight, serum estradiol (E2), total cholesterol and triglyceride levels, uteri weight and measurement of bone mineral density (BMD) were conducted.Results The EtOAc and n-BuOH fractions showed the most potent inhibitory activity on PTH-induced bone resorption. Further research using OVX rat model revealed that the n-BuOH fraction significantly prevented BMD loss due to OVX operation. While, the uteri weight and serum estradiol (E2), total cholesterol and triglyceride levels displayed no differences compared with those of control group (OVX rats), suggesting the n-BuOH fraction should have no estrogen-like side effects.Conclusions The results reveal that the n-BuOH soluble fraction of the root of Achyranthes bidentata is effective at preventing bone loss in OVX rats and has a great potential as an alternative tool for the treatment of osteoporosis.

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Crossref · 10.1016/j.jep.2009.11.016

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Authors
He, C.-C., Hui, R.-R., Tezuka, Y., Kadota, S., & Li, J.-X.
Affiliations
  • Nanjing University (CN)
  • University of Toyama (JP)
Corresponding author
Li, Jian-Xin
Venue
Journal of Ethnopharmacology
Publisher
Elsevier BV
Published
2010-02
Volume
127
Issue
2
Pages
229-234
ISSN
0378-8741 (ISSN-L)
OpenAlex
W2041564990
Cited by
67
References
28
Citations by year
2026: 3 · 2025: 3 · 2024: 5 · 2023: 7 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 6 · 2017: 5 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 10
FWCI
4.75
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Ethnopharmacology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Science Fund for Creative Research Groups
Topics
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
Keywords
Achyranthes bidentata, osteoprotective effect, ovariectomy, bone resorption, parathyroid hormone, n-butanol fraction, ethyl acetate fraction, bone mineral density, osteoporosis, Chinese herbal medicine, root extract
MeSH terms
Animals, Animals, Newborn, Bone Resorption, Dose-Response Relationship, Drug, Female, Humans, Osteoporosis, Ovariectomy, Plant Extracts, Bone Density, Rats, Wistar, Plant Roots, Achyranthes, Mice, Rats

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