Osteoprotective effect of extract from Achyranthes bidentata in ovariectomized rats
Abstract
Aim of the study A Chinese herbal drug, root of Achyranthes bidentata showed a potent inhibitory activity on bone resorption induced by parathyroid hormone (PTH) in a bone organ culture using neonatal mouse parietal bones. The present study is to clarify the fractions responsible for the activity and further explore the osteoprotective effect of the fraction in vivo.Materials and methods The hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH) and water soluble fractions of methanol extract of the root of Achyranthes bidentata were prepared and screened for their anti-bone resorption activity using the bone organ culture system. The n-BuOH soluble fraction was further administered orally at doses of 25, 50 and 100mg/(kgday) to ovariectomized (OVX) rats. The analyses of the rat body weight, serum estradiol (E2), total cholesterol and triglyceride levels, uteri weight and measurement of bone mineral density (BMD) were conducted.Results The EtOAc and n-BuOH fractions showed the most potent inhibitory activity on PTH-induced bone resorption. Further research using OVX rat model revealed that the n-BuOH fraction significantly prevented BMD loss due to OVX operation. While, the uteri weight and serum estradiol (E2), total cholesterol and triglyceride levels displayed no differences compared with those of control group (OVX rats), suggesting the n-BuOH fraction should have no estrogen-like side effects.Conclusions The results reveal that the n-BuOH soluble fraction of the root of Achyranthes bidentata is effective at preventing bone loss in OVX rats and has a great potential as an alternative tool for the treatment of osteoporosis.
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- Authors
- He, C.-C., Hui, R.-R., Tezuka, Y., Kadota, S., & Li, J.-X.
- Affiliations
- Nanjing University (CN)
- University of Toyama (JP)
- Corresponding author
- Li, Jian-Xin
- Venue
- Journal of Ethnopharmacology
- Publisher
- Elsevier BV
- Published
- 2010-02
- Volume
- 127
- Issue
- 2
- Pages
- 229-234
- ISSN
- 0378-8741 (ISSN-L)
- OpenAlex
- W2041564990
- Cited by
- 67
- References
- 28
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 5 · 2023: 7 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 6 · 2017: 5 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 10
- FWCI
- 4.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Ethnopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Science Fund for Creative Research Groups
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- Achyranthes bidentata, osteoprotective effect, ovariectomy, bone resorption, parathyroid hormone, n-butanol fraction, ethyl acetate fraction, bone mineral density, osteoporosis, Chinese herbal medicine, root extract
- MeSH terms
- Animals, Animals, Newborn, Bone Resorption, Dose-Response Relationship, Drug, Female, Humans, Osteoporosis, Ovariectomy, Plant Extracts, Bone Density, Rats, Wistar, Plant Roots, Achyranthes, Mice, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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