In vitro screening on amyloid precursor protein modulation of plants used in Ayurvedic and Traditional Chinese medicine for memory improvement
Abstract
Ethnopharmacological relevance The 15 herbs for the screening have been traditionally used in Ayurvedic medicine or in Traditional Chinese medicine (TCM) for the treatment of cognitive disorders clinically.Aim of the study Fifteen plant species were investigated for the inhibition of amyloid peptide (Aβ) production and modulation of amyloid precursor protein (APP) processing.Materials and methods The selected plants were extracted successively with 70% ethyl alcohol and absolute alcohol concentrated with rotary evaporation then lyophilized. Using a mouse neuroblastoma cells expressing Swedish APP (N2a-SweAPP), MTT assay was performed to determine the toxicity concentration of each herbal extract. In order to evaluate the activity of ethanol extracts on Aβ inhibition, the N2a-SweAPP cells were treated with a high and low dosage of different extracts for 24h, Aβs levels in the supernatant of conditioned media were assessed by ELISA. The most active extracts were then subjected to test the effect on APP modulation in N2a-SweAPP cells by determining their effect on sAPPα and sAPPβ through western blot analysis.Results Among the screened herbal extracts, only Polygonum multiflorum Thunb. (root) and Convolvulus pluricaulis Choisy. (leaves) showed profound inhibition of Aβ production. MTT assay demonstrated that the anti-Aβ effect of these extracts was not a sequential consequence of their cytotoxicity. The extract of Polygonum multiflorum Thunb. (root) could reduce Aβ production only through APP modulation, which was exhibited together with the up-regulation of sAPPα and down-regulation of sAPPβ.Conclusion The results show that extract of Polygonum multiflorum Thunb. (root) can lower Aβ generation by modulating APP processing in the N2a-SwedAPP cell line. These results corroborate the traditional use of Polygonum multiflorum Thunb. (root) for the treatment of cognitive disorders including Alzheimer's disease (AD).
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Alcoholic extract of C. pluricaulis Choisy (leaves) showed Aβ production inhibition in-vitro.
“Alcoholic extract of C. pluricaulis Choisy (leaves) showed Aβ production inhibition in-vitro [243].”
From Indian Medicinal Herbs and Formulations for Alzheimer’s Disease, from Traditional Knowledge to Scientific Assessment (2020) · cites this paper as [243]
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References 43 · Cited by 29
- Authors
- Liu, L.-F., Durairajan, S. S. K., Lu, J.-H., Koo, I., & Li, M.
- Affiliations
- Hong Kong Baptist University (HK)
- Corresponding author
- Li, Min
- Venue
- Journal of Ethnopharmacology
- Publisher
- Elsevier BV
- Published
- 2012-06
- Volume
- 141
- Issue
- 2
- Pages
- 754-760
- ISSN
- 0378-8741 (ISSN-L)
- OpenAlex
- W2071308173
- Cited by
- 29
- References
- 43
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2021: 1 · 2020: 4 · 2018: 3 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 4.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Ethnopharmacology (publishedVersion)
- PubMed (publishedVersion)
- HKBU Institutional Repository (Hong Kong Baptist University) (submittedVersion)
- Funders
- Hong Kong Baptist University
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
- Keywords
- amyloid precursor protein, Alzheimer's disease, Polygonum multiflorum, Convolvulus pluricaulis, plant extracts, APP processing, sAPPα, sAPPβ, cognitive disorders, Ayurveda, traditional Chinese medicine, memory enhancement
- MeSH terms
- Ethanol, Animals, Cell Survival, Dose-Response Relationship, Drug, Drugs, Chinese Herbal, Enzyme-Linked Immunosorbent Assay, Medicine, Ayurvedic, Medicine, Chinese Traditional, Memory, Mutation, Neurons, Plants, Medicinal, Protein Processing, Post-Translational, Solvents, Transfection, Blotting, Western, Amyloid beta-Peptides, Amyloid beta-Protein Precursor, Plant Leaves, Plant Roots, Nootropic Agents, Polygonum, Convolvulus, Withania, Cell Line, Tumor, Mice
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