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Cited by 50Natural product bioactivities and synthesisHepatitis B Virus StudiesPhytochemical Studies and Bioactivities

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Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpioides Lam

Huang, Quanfang, Zhang, Shijun, Huang, Renbin, Wei, Ling, Chen, Yongxing, Lv, Shujuan, Liang, Chunhong, Tan, Shimei, liang, Shuang, Zhuo, Lang, Lin, Xing·2013·Journal of Ethnopharmacology
DOI 10.1016/j.jep.2013.09.009
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Abstract

Ethnopharmacological relevance Hydrocotyle sibthorpioides (Apiaceae) have been used as a folk remedy for the treatment of fever, edema, detoxication, throat pain, psoriasis and hepatitis B virus infections in China. The aim of this study is to isolate and identify an anti-HBV compound from this herb. Materials and methods A compound (saponin) was isolated from the active ethanol extract using bioassay-guided screening. The structure of the saponin was elucidated by spectroscopic methods and compared with published data. The anti-HBV activity of the saponin was evaluated by detecting the levels of HBV antigens, extracellular HBV DNA, nuclear covalent closed circular DNA (cccDNA) and five HBV promoters in HepG2.2.15 cells. In addition, the levels of serum HBsAg/HBeAg, DHBV DNA, ALT/AST and hepatic pathological changes were analyzed in DHBV-infected ducks. Results The chemical analysis indicated that the saponin isolated from Hydrocotyle sibthorpioides is asiaticoside. The pharmacodynamics experimental studies showed that asiaticoside effectively suppressed the levels of HBsAg/HBeAg, extracellular HBV DNA and intracellular cccDNA in a dose-dependent manner. Furthermore, experiments demonstrated that asiaticoside markedly reduced viral DNA transcription and replication by inhibiting the activities of core, s1, s2, and X gene promoters. In addition, asiaticoside markedly reduced DHBV replication without any obvious signs of toxicity. The levels of serum DHBV DNA, HBsAg/HBeAg were…

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Metadata

Authors
Huang, Q., Zhang, S., Huang, R., Wei, L., Chen, Y., Lv, S., Liang, C., Tan, S., liang, S., Zhuo, L., & Lin, X.
Affiliations
  • The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine (CN)
  • Guangxi Medical University (CN)
  • Institute of Bioengineering and Nanotechnology (SG)
Corresponding author
Lin, Xing
ORCID
Venue
Journal of Ethnopharmacology
Publisher
Elsevier BV
Published
2013-11
Volume
150
Issue
2
Pages
568-575
ISSN
0378-8741 (ISSN-L)
DOI
10.1016/j.jep.2013.09.009
OpenAlex
W1988761020
Cited by
50
References
1
Citations by year
2026: 1 · 2025: 2 · 2023: 17 · 2022: 10 · 2021: 2 · 2020: 6 · 2019: 4 · 2018: 1 · 2016: 5 · 2015: 2
FWCI
1.05
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Ethnopharmacology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (81260674, 81260505)
  • Foundation of Guangxi Key Laboratory for the Prevention & Treatment of Regional High-Incidence Diseases (KFJJ2010-71, KFJJ2011-37)
  • Guangxi Scientific Research & Technology Development Research (10124008-6, 0992003A-2)
  • Guangxi Science and Technology Foundation Platform (12-97-20)
Topics
  • Physical Sciences › Chemistry › Organic Chemistry › Natural product bioactivities and synthesis
  • Health Sciences › Medicine › Epidemiology › Hepatitis B Virus Studies
  • Physical Sciences › Chemistry › Organic Chemistry › Phytochemical Studies and Bioactivities
Keywords
Hydrocotyle sibthorpioides, asiaticoside, anti-HBV activity, hepatitis B virus, hepatitis delta virus, saponins, covalently closed circular DNA, viral DNA replication, liver injury, traditional Chinese medicine, bioassay-guided isolation
MeSH terms
Alanine Transaminase, Animals, Antiviral Agents, Aspartate Aminotransferases, DNA, Viral, Ducks, Hepatitis B e Antigens, Hepatitis B Surface Antigens, Hepatitis B virus, Hepatitis, Viral, Animal, Humans, Liver, Plant Extracts, Saponins, Triterpenes, Virus Replication, Hepatitis B Virus, Duck, Hepadnaviridae Infections, Centella, Hep G2 Cells

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CoChat. (2026). Citation check: Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpioides Lam. https://app.cochat.ai/papers/10.1016/j.jep.2013.09.009
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Huang, Q., Zhang, S., Huang, R., Wei, L., Chen, Y., Lv, S., Liang, C., Tan, S., liang, S., Zhuo, L., & Lin, X. (2013). Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpioides Lam. Journal of Ethnopharmacology. https://doi.org/10.1016/j.jep.2013.09.009