Protective effects of Asiaticoside on acute liver injury induced by lipopolysaccharide/D-galactosamine in mice
Abstract
Asiaticoside (AS), a triterpenoid product isolated from Centella asiatica, has been described to exhibit anti-in fl ammatory activities in several inflammatory models. However, the effects of AS on liver injury are poorly understood. The present study was undertaken to investigate whether AS is efficacious against Lipopolysaccharide (LPS) /D-galactosamine (D-GalN)-induced acute liver injury in mice and its potential mechanisms. AS (5, 10 and 20 mg/kg/d) was pretreated orally once daily for 3 days before LPS/D-GalN injected in mice. The mortality, hepatic tissue histology, plasma levels of Tumor necrosis factor-alpha (TNF-alpha) and alanine aminotransferase (ALT) and aspartate aminotransferase (AST), hepatic tissue TNF-alpha and caspase-3 activity were measured. Besides, western blotting analysis of phospho-p38 mitogen-activated protein kinase (phospho-p38 MAPK), phospho-c-jun N-terminal kinase (phospho-JNK) and phospho-extracellular signal regulated kinase (phospho-ERK) were determined. As a result, AS showed significant protection as evidenced by the decrease of elevated aminotransferases, hepatocytes apoptosis and caspase-3, alleviation of mortality and improvement of liver pathological injury in a dose-dependent manner. Further, we found that AS dose-dependently reduced the elevation of phospho-p38 MAPK, phospho-JNK, phospho-ERK protein and TNF-alpha mRNA expression in liver tissues and plasma TNF-alpha. These results suggest that AS has remarkable hepatoprotective effects…
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Asiaticoside and madecassoside are promising hepatoprotective agents for acute liver injury or liver failure.
“Thus asiaticoside and madecassoside are promising hepatoprotective agents for acute liver injury or liver failure.”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [105]
Asiaticoside and madecassoside show significant protection against LPS/D-GalN-induced acute liver injury.
“Asiaticoside and madecassoside have shown significant protection against lipopolysaccharide (LPS)/D‐galactosamine (D‐GaIN)‐induced acute liver injury in two separate studies.”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [105]
Asiaticoside dose-dependently reduces elevated aminotransferases, hepatocyte apoptosis and caspase-3 in LPS/D-GalN-induced acute liver injury.
“dose‐dependently reduced elevated aminotransferases, hepatocytes apoptosis and caspase‐3”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [105]
Asiaticoside has anti-inflammatory effects in LPS/D-GalN-induced acute liver injury.
“Asiaticoside presented anti‐inflammatory effects”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [105]
Asiaticoside inhibits expression of TNF-alpha and MAPKs to protect against hepatic damage.
“Furthermore, asiaticoside inhibited the expression of TNF‐alpha and MAPKs to protect against hepatic damage.”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [105]
Asiaticoside improves mortality and liver pathological injury in LPS/D-GalN-induced acute liver injury.
“as well as improved mortality and liver pathological injury.”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [105]
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Biosynthesis and bioactivities of triterpenoids from Centella asiatica: Challenges and opportunities
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- Authors
- Zhang, L., Li, H.-Z., Gong, X., Luo, F.-L., Wang, B., Hu, N., Wang, C.-D., Zhang, Z., & Wan, J.-Y.
- Affiliations
- Chongqing Medical University (CN)
- The Affiliated Yongchuan Hospital of Chongqing Medical University (CN)
- Venue
- Phytomedicine
- Publisher
- Elsevier BV
- Published
- 2010-08
- Volume
- 17
- Issue
- 10
- Pages
- 811-819
- ISSN
- 0944-7113 (ISSN-L)
- OpenAlex
- W2076944566
- Cited by
- 79
- References
- 0
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 1 · 2023: 5 · 2022: 5 · 2021: 7 · 2020: 4 · 2018: 2 · 2017: 7 · 2016: 9 · 2015: 7 · 2014: 7 · 2013: 5 · 2012: 3
- FWCI
- 5.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Phytomedicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal plant effects and applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Keywords
- asiaticoside, Centella asiatica, triterpenoids, hepatoprotection, acute liver injury, TNF-α, MAPK signaling, p38 MAPK, JNK, ERK, hepatocyte apoptosis, caspase-3, inflammation
- MeSH terms
- Animals, Base Sequence, Galactosamine, Lipopolysaccharides, Mice, Inbred BALB C, Triterpenes, Blotting, Western, Polymerase Chain Reaction, DNA Primers, MAP Kinase Signaling System, Mice, Chemical and Drug Induced Liver Injury
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