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Cited by 354Fungal Biology and ApplicationsTraditional Chinese Medicine AnalysisAgricultural and Biological Research

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Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum

Chen, Yi, Xie, Ming-Yong, Gong, Xiao-Feng·2007·Journal of Food Engineering
DOI 10.1016/j.jfoodeng.2006.10.018
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Abstract

In this paper, a method of microwave-assisted extraction (MAE) was for the first time used to extract total triterpenoid saponins from Ganoderma atrum, which have been reported to have several medicinal properties and uses. The extracts were directly determined by colorimetric method without any further treatment. Compared with shaking extraction method, heat reflux extraction, supercritical fluid carbon dioxide extraction (SFE) and normal ultrasound-assisted extraction (UAE), MAE only need 5 min to give the highest yield of triterpenoid saponins at 0.968%, while the other extraction methods need several hours or even more than 10 h and give lower yield. Several factors affecting the MAE extraction rate were also discussed, such as extraction time, temperature, ratio of solvent to material and the kind of solvents. Optimal conditions of MAE can be concluded as follows: 5 min at 90 °C, the ratio of solvent to material is 25 by using 95% ethanol as the solvent.

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Metadata

Authors
Chen, Y., Xie, M.-Y., & Gong, X.-F.
Affiliations
Nanchang University (CN)
ORCID
Chen, Yi
Venue
Journal of Food Engineering
Publisher
Elsevier BV
Published
2007-07
Volume
81
Issue
1
Pages
162-170
ISSN
0260-8774 (ISSN-L)
DOI
10.1016/j.jfoodeng.2006.10.018
OpenAlex
W2088133007
Cited by
354
References
0
Citations by year
2026: 12 · 2025: 21 · 2024: 17 · 2023: 20 · 2022: 19 · 2021: 20 · 2020: 22 · 2019: 23 · 2018: 24 · 2017: 24 · 2016: 16 · 2015: 25 · 2014: 17 · 2013: 18 · 2012: 21
FWCI
8.21
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
Journal of Food Engineering (publishedVersion)
Funders
  • Department of Science and Technology, Ministry of Science and Technology, India
  • Jiangxi Provincial Department of Science and Technology
Topics
  • Health Sciences › Medicine › Pharmacology (medical) › Fungal Biology and Applications
  • Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
Keywords
microwave-assisted extraction, triterpenoid saponins, extraction yield, extraction optimization, ethanol solvent, supercritical CO2 extraction, heat reflux extraction

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CoChat. (2026). Citation check: Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum. https://app.cochat.ai/papers/10.1016/j.jfoodeng.2006.10.018
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Chen, Y., Xie, M.-Y., & Gong, X.-F. (2007). Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum. Journal of Food Engineering. https://doi.org/10.1016/j.jfoodeng.2006.10.018