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Cited by 95Genetic diversity and population structurePhytochemicals and Medicinal PlantsGenetic Mapping and Diversity in Plants and Animals

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Diversity of the genus Ocimum (Lamiaceae) through morpho-molecular (RAPD) and chemical (GC–MS) analysis

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Chowdhury, Tanmay, Mandal, Amitava, Roy, Subhas Chandra, De Sarker, Dilip·2017·Journal of Genetic Engineering and Biotechnology

Abstract

In this present study, we have described the diversity of nine Ocimum genotypes naturally grown in the Dakshin Dinajpur district of West Bengal, India. Their diversity was determined on the basis of morphological, chemical and randomly amplified polymorphic DNA (RAPD) to determine the level of variation present in the genus Ocimum. Among nine Ocimum genotypes six (O. americanum, O. × africanum, O. basilicum, O. gratissimum, O. kilimandscharicum and O. tenuiflorum) are found to be different Ocimum species and the rest are as varieties. A total of 18 qualitative and 17 quantitative morphological traits and chemical compositions were evaluated. Significant variations were observed in the morphological traits except O. × africanum and O. basilicum species. Cluster generated from the morphological data showed two different groups viz. basilicum group and sanctum group. Chemical analysis did not show much variation between morphologically similar species viz. O. × africanum and O. basilicum. However, RAPD analyses clearly showed that O. × africanum and O. basilicum are different species. Thus the combined analyses of morphological traits, chemical and molecular markers represent the best possible approach to confirm taxonomic delineation. Moreover, we are reporting O. × africanum for the first time from this region as well as from West Bengal, India.

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Crossref · 10.1016/j.jgeb.2016.12.004

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Figures

Fig. 1 Dissimilarity dendrogram generated by Ward’s method showing major clusters among nine Ocimum genotypes based on their morphological traits. OTP-O. tenuiflorum (Krishna tulsi); OTG- O. tenuiflorum (Radha tulsi); OG-O. gratissimum (Ram tulsi); OG1-Ocimum gratissimum (Ajowan tulsi); O×A-O. × africanum (Lebu tulsi); OB-O. basilicum (Babu tulsi); OB1-O. basilicum (Marua); OA-O. americanum (Bon tulsi); OK-O. kilimandscharicum (Karpur tulsi).
Fig. 1 Dissimilarity dendrogram generated by Ward’s method showing major clusters among nine Ocimum genotypes based on their morphological traits. OTP-O. tenuiflorum (Krishna tulsi); OTG- O. tenuiflorum (Radha tulsi); OG-O. gratissimum (Ram tulsi); OG1-Ocimum gratissimum (Ajowan tulsi); O×A-O. × africanum (Lebu tulsi); OB-O. basilicum (Babu tulsi); OB1-O. basilicum (Marua); OA-O. americanum (Bon tulsi); OK-O. kilimandscharicum (Karpur tulsi).CC BY-NC-ND · PubMed Central
Fig. 2 RAPD band profiling was visualized on 1.5% agarose gel electrophoresis after stained with ethidium bromide in nine Ocimum genotypes and photograph for analysis. a-BGM-1, b-BGM-3, c-BGM-4 & d-BGM-7. M-Marker (100-3000 bp ladder); lane 1-Ocimum gratissimum L.; 2-O. × africanum Lour.; 3 -Ocimum basilicum L.; 4 -Ocimum tenuiflorum L. (Purple); 5 -Ocimum basilicum L. (Marua); 6 -Ocimum americanum L. ; 7 -Ocimum tenuiflorum L. (Green); 8 -Ocimum gratissimum L. (Ajowan); 9 -Ocimum kilimandscharicum Guerke.
Fig. 2 RAPD band profiling was visualized on 1.5% agarose gel electrophoresis after stained with ethidium bromide in nine Ocimum genotypes and photograph for analysis. a-BGM-1, b-BGM-3, c-BGM-4 & d-BGM-7. M-Marker (100-3000 bp ladder); lane 1-Ocimum gratissimum L.; 2-O. × africanum Lour.; 3 -Ocimum basilicum L.; 4 -Ocimum tenuiflorum L. (Purple); 5 -Ocimum basilicum L. (Marua); 6 -Ocimum americanum L. ; 7 -Ocimum tenuiflorum L. (Green); 8 -Ocimum gratissimum L. (Ajowan); 9 -Ocimum kilimandscharicum Guerke.CC BY-NC-ND · PubMed Central
Fig. 3 Dendrogram represented inter and intra specific relationship among nine genotypes of the Ocimum based on RAPD similarity matrix.
Fig. 3 Dendrogram represented inter and intra specific relationship among nine genotypes of the Ocimum based on RAPD similarity matrix.CC BY-NC-ND · PubMed Central

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Citations

Cites

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Research works printed but not linked: 19

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Metadata

Authors
Chowdhury, T., Mandal, A., Roy, S. C., & De Sarker, D.
Affiliations
  • Raiganj University (IN)
  • North Bengal University (IN)
Corresponding author
Chowdhury, Tanmay
Venue
Journal of Genetic Engineering and Biotechnology
Publisher
Elsevier BV
Published
2017-06
Volume
15
Issue
1
Pages
275-286
ISSN
1687-157X (ISSN-L)
OpenAlex
W2568652151
Cited by
95
References
33
Citations by year
2026: 5 · 2025: 9 · 2024: 10 · 2023: 20 · 2022: 17 · 2021: 13 · 2020: 9 · 2019: 3 · 2018: 7 · 2017: 1
FWCI
3.63
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Genetic Engineering and Biotechnology (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Raiganj University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
  • Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
Keywords
Ocimum, Lamiaceae, species diversity, Ocimum basilicum, morphological variation, RAPD markers, essential oil composition, West Bengal

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