Biodegradable and conventional microplastics exhibit distinct microbiome, functionality, and metabolome changes in soil

Abstract

Environmental concerns with liberal petroleum-based plastic use have led to demand for sustainable biodegradable alternatives. However, the inadequate end-of-life treatment of plastics may emit microplastics, either conventional or biodegradable, to the terrestrial environment. It is essential to evaluate the possible effects of conventional and biodegradable microplastics on the composition and function of soil microbial communities. Therefore, we conducted a soil microcosm experiment with polyethylene (PE), polystyrene (PS), polylactide (PLA), or polybutylene succinate (PBS) microplastics. The soil microbiome and metabolome were evaluated via 16S rRNA gene sequencing, metagenomics, and untargeted metabolomics. We reported that the presence of conventional or biodegradable microplastics can significantly alter soil microbial community composition. Compared to the control soils, the microbiome in PBS and PLA amended soils exhibited higher potential for uptake of exogenous carbohydrates and amino acids, but a reduced capacity for related metabolic function, potentially due to catabolite repression. No differences in soil metabolome can be observed between conventional microplastic treatments and the control. The potential reason may be that the functional diversity was unaffected by PE and PS microplastics, while the biodegradable particles promoted the soil microbial multifunctionality. Our findings systematically shed light on the influence of conventional and biodegradable microplastics on soil microorganisms, facilitating microplastic regulation.