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Cited by 110Viral gastroenteritis research and epidemiologyBacterial Identification and Susceptibility TestingParasitic Infections and Diagnostics

The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.

Systematic application of multiplex PCR enhances the detection of bacteria, parasites, and viruses in stool samples

McAuliffe, Gary N., Anderson, Trevor P., Stevens, Mary, Adams, Jacqui, Coleman, Robyn, Mahagamasekera, Patalee, Young, Sheryl, Henderson, Tom, Hofmann, Maria, Jennings, Lance C., Murdoch, David R.·2013·Journal of Infection
DOI 10.1016/j.jinf.2013.04.009
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Abstract

Objectives To determine whether systematic testing of faecal samples with a broad range multiplex PCR increases the diagnostic yield in patients with diarrhoea compared with conventional methods and a clinician initiated testing strategy.Methods 1758 faecal samples from 1516 patients with diarrhoea submitted to two diagnostic laboratories were tested for viral, bacterial, and parasitic pathogens by Fast-Track Diagnostics multiplex real-time PCR kits and conventional diagnostic tests.Results Multiplex PCR detected pathogens in 530 samples (30%): adenovirus (51, 3%), astrovirus (95, 5%), norovirus (172, 10%), rotavirus (3, 0.2%), Campylobacter jejuni/coli (85, 5%), Salmonella spp. (22, 1%), Clostridium difficile (72, 4%), entero-haemorrhagic Escherichia coli (21, 1%), Cryptosporidium spp. (3, 0.2%), Entamoeba histolytica (1, 0.1%), and Giardia lamblia (59, 3%). In contrast, conventional testing detected a pathogen in 324 (18%) samples.Conclusions Using a systematic approach to the diagnosis of gastroenteritis improved diagnostic yield. This enhanced detection with PCR was achieved by a combination of improved detection of individual pathogens and detection of pathogens not requested or unable to be tested by conventional tests. This approach also allowed earlier identification for most pathogens and created a workflow which is likely to adapt well for many diagnostic laboratories.

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Crossref · 10.1016/j.jinf.2013.04.009

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Metadata

Authors
McAuliffe, G. N., Anderson, T. P., Stevens, M., Adams, J., Coleman, R., Mahagamasekera, P., Young, S., Henderson, T., Hofmann, M., Jennings, L. C., & Murdoch, D. R.
Affiliations
Canterbury Health Laboratories (NZ)
Corresponding author
McAuliffe, Gary N.
ORCID
Venue
Journal of Infection
Publisher
Elsevier BV
Published
2013-08
Volume
67
Issue
2
Pages
122-129
ISSN
0163-4453 (ISSN-L)
DOI
10.1016/j.jinf.2013.04.009
OpenAlex
W2063754512
Cited by
110
References
32
Citations by year
2026: 3 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 10 · 2021: 4 · 2020: 10 · 2019: 8 · 2018: 10 · 2017: 16 · 2016: 10 · 2015: 15 · 2014: 11 · 2013: 5
FWCI
9.95
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Journal of Infection (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
  • Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
Keywords
multiplex PCR, diarrhea, fecal samples, pathogen detection, gastroenteritis, norovirus, rotavirus, adenovirus, astrovirus, Campylobacter, Salmonella, Clostridium difficile, Cryptosporidium, Entamoeba histolytica, Giardia lamblia, diagnostic yield, real-time PCR
MeSH terms
Adolescent, Adult, Aged, Aged, 80 and over, Animals, Bacteria, Child, Child, Preschool, Diarrhea, Feces, Female, Gastroenteritis, Humans, Infant, Infant, Newborn, Male, Middle Aged, Parasites, Sensitivity and Specificity, Time Factors, Viruses, Molecular Diagnostic Techniques, Young Adult, Multiplex Polymerase Chain Reaction

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