The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Systematic application of multiplex PCR enhances the detection of bacteria, parasites, and viruses in stool samples
Abstract
Objectives To determine whether systematic testing of faecal samples with a broad range multiplex PCR increases the diagnostic yield in patients with diarrhoea compared with conventional methods and a clinician initiated testing strategy.Methods 1758 faecal samples from 1516 patients with diarrhoea submitted to two diagnostic laboratories were tested for viral, bacterial, and parasitic pathogens by Fast-Track Diagnostics multiplex real-time PCR kits and conventional diagnostic tests.Results Multiplex PCR detected pathogens in 530 samples (30%): adenovirus (51, 3%), astrovirus (95, 5%), norovirus (172, 10%), rotavirus (3, 0.2%), Campylobacter jejuni/coli (85, 5%), Salmonella spp. (22, 1%), Clostridium difficile (72, 4%), entero-haemorrhagic Escherichia coli (21, 1%), Cryptosporidium spp. (3, 0.2%), Entamoeba histolytica (1, 0.1%), and Giardia lamblia (59, 3%). In contrast, conventional testing detected a pathogen in 324 (18%) samples.Conclusions Using a systematic approach to the diagnosis of gastroenteritis improved diagnostic yield. This enhanced detection with PCR was achieved by a combination of improved detection of individual pathogens and detection of pathogens not requested or unable to be tested by conventional tests. This approach also allowed earlier identification for most pathogens and created a workflow which is likely to adapt well for many diagnostic laboratories.
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“According to a previous report, when multiplex molecular analysis and conventional diagnostic test were compared for detection of viruses, bacteria, and parasites in 1,758 stool samples collected from 1,516 patients, molecular analysis identified pathogens in 530 samples (30%) whereas conventional test identified pathogens in only 324 samples (18%) [22].”
From Guideline for the Antibiotic Use in Acute Gastroenteritis (2019) · cites this paper as [22]
In the citing paper
“here, although enzyme-based immunological assays take a short amount of time, they have relatively low sensitivity and specificity [22].”
From Guideline for the Antibiotic Use in Acute Gastroenteritis (2019) · cites this paper as [22]
Citations
Cites
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Kosek, M. N., Bern, C., & Guerrant, R. L. (2003) · Europe PMC (PubMed Central) · Cited by 1,617
The Emerging Clinical Importance of Non-O157 Shiga Toxin--Producing Escherichia coli
Hughes, J. M., Wilson, M. E., Johnson, K. E., et al. (2006) · Clinical Infectious Diseases · doi:10.1086/509573 · Cited by 488
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Viruses causing gastroenteritis
Wilhelmi, I., Román, E., & Sánchez-Fauquier, A. (2003) · Clinical Microbiology and Infection · doi:10.1046/j.1469-0691.2003.00560.x · Cited by 401
Taniuchi, M., Verweij, J. J., Noor, Z., Sobuz, S. U., van Lieshout, L., Petri, W. A., Haque, R., & Houpt, E. R. (2011) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2011.10-0461 · Cited by 199
Logan, C., O’Leary, J. J., & O’Sullivan, N. (2006) · Journal of Clinical Microbiology · doi:10.1128/jcm.00915-06 · Cited by 195
Progress in understanding norovirus epidemiology
Koopmans, M. (2008) · Current Opinion in Infectious Diseases · doi:10.1097/qco.0b013e3283108965 · Cited by 161
Improved Detection of Five Major Gastrointestinal Pathogens by Use of a Molecular Screening Approach
de Boer, R. F., Ott, A., Kesztyüs, B., et al. (2010) · Journal of Clinical Microbiology · doi:10.1128/jcm.01124-10 · Cited by 151
Ozaki, E., Kato, H., Kita, H., Karasawa, T., Maegawa, T., Koino, Y., Matsumoto, K., Takada, T., Nomoto, K., Tanaka, R., et al. (2004) · Journal of Medical Microbiology · doi:10.1099/jmm.0.05376-0 · Cited by 144
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ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults
Riddle, M. S., DuPont, H. L., & Connor, B. A. (2016) · American Journal of Gastroenterology · doi:10.1038/ajg.2016.126 · Cited by 435
Guideline for the Antibiotic Use in Acute Gastroenteritis
Kim, Y. J., Park, K.-H., Park, D.-A., Park, J., Bang, B. W., Lee, S. S., Lee, E. J., Lee, H.-J., Hong, S. K., & Kim, Y. R. (2019) · Infection & Chemotherapy · doi:10.3947/ic.2019.51.2.217 · Cited by 46
“, are selectively cultured, and only certain viruses, such as norovirus and rotavirus, are tested in enzyme-based immunological assays; here, although enzyme-based immunological assays take a short amount of time, they have relatively low sensitivity and specificity [22].”
BackgroundMethodAbbott, A. N., & Fang, F. C. (2017) · Clinical Microbiology Newsletter · doi:10.1016/j.clinmicnews.2017.08.004 · Cited by 13
Metadata
- Authors
- McAuliffe, G. N., Anderson, T. P., Stevens, M., Adams, J., Coleman, R., Mahagamasekera, P., Young, S., Henderson, T., Hofmann, M., Jennings, L. C., & Murdoch, D. R.
- Affiliations
- Canterbury Health Laboratories (NZ)
- Corresponding author
- McAuliffe, Gary N.
- Venue
- Journal of Infection
- Publisher
- Elsevier BV
- Published
- 2013-08
- Volume
- 67
- Issue
- 2
- Pages
- 122-129
- ISSN
- 0163-4453 (ISSN-L)
- OpenAlex
- W2063754512
- Cited by
- 110
- References
- 32
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 10 · 2021: 4 · 2020: 10 · 2019: 8 · 2018: 10 · 2017: 16 · 2016: 10 · 2015: 15 · 2014: 11 · 2013: 5
- FWCI
- 9.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Infection (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
- Keywords
- multiplex PCR, diarrhea, fecal samples, pathogen detection, gastroenteritis, norovirus, rotavirus, adenovirus, astrovirus, Campylobacter, Salmonella, Clostridium difficile, Cryptosporidium, Entamoeba histolytica, Giardia lamblia, diagnostic yield, real-time PCR
- MeSH terms
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Bacteria, Child, Child, Preschool, Diarrhea, Feces, Female, Gastroenteritis, Humans, Infant, Infant, Newborn, Male, Middle Aged, Parasites, Sensitivity and Specificity, Time Factors, Viruses, Molecular Diagnostic Techniques, Young Adult, Multiplex Polymerase Chain Reaction
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