The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies
Open accessAbstract
Complex protein networks are involved in nearly all cellular processes. To uncover these vast networks of protein interactions, various high-throughput screening technologies have been developed. Over the last decade, bimolecular fluorescence complementation (BiFC) assay has been widely used to detect protein-protein interactions (PPIs) in living cells. This technique is based on the reconstitution of a fluorescent protein in vivo. Easy quantification of the BiFC signals allows effective cell-based high-throughput screenings for protein-binding partners and drugs that modulate PPIs. Recently, with the development of large screening libraries, BiFC has been effectively applied for genome-wide PPI studies and has uncovered novel protein interactions, providing new insight into protein functions. In this review, we describe the development of reagents and methods used for BiFC-based screens in yeast, plants, and mammalian cells. We also discuss the advantages and drawbacks of these methods and highlight the application of BiFC in large-scale studies.
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Cites
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Self-citationThe Genetic Landscape of a Cell
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Müller, M., Gräbnitz, F., Barandun, N., Shen, Y., Wendt, F., Steiner, S. N., Severin, Y., Vetterli, S. U., Mondal, M., Prudent, J. R., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-27280-x · Cited by 101
Mamouei, Z., Zeng, G., Wang, Y., & Wang, Y. (2017) · Molecular Microbiology · doi:10.1111/mmi.13864 · Cited by 49
A Live Cell Protein Complementation Assay for ORFeome-Wide Probing of Human HOX Interactomes
Jia, Y., Reboulet, J., Gillet, B., Hughes, S., Forcet, C., Tribollet, V., Hajj Sleiman, N., Kundlacz, C., Vanacker, J.-M., Bleicher, F., et al. (2023) · Cells · doi:10.3390/cells12010200 · Cited by 10
Metadata
- Authors
- Miller, K. E., Kim, Y., Huh, W.-K., & Park, H.-O.
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 2015-06
- Volume
- 427
- Issue
- 11
- Pages
- 2039-2055
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W2058687710
- Cited by
- 250
- References
- 8
- FWCI
- 11.78
- Type
- journal article
- Language
- en
- Open access
- green
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- Miller, K. E., Kim, Y., Huh, W.-K., & Park, H.-O. (2015). Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies. Journal of Molecular Biology. https://doi.org/10.1016/j.jmb.2015.03.005
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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