Förster Resonance Energy Transfer Methods for Quantification of Protein–Protein Interactions on Microarrays
Abstract
Methods based on Förster (or fluorescence) resonance energy transfer (FRET) are widely used in various areas of bioanalysis and molecular biology, such as fluorescence microscopy, quantitative real-time polymerase chain reaction (PCR), immunoassays, or enzyme activity assays, just to name a few. In the last years, these techniques were successfully implemented to multiplex biomolecular screening on microarrays. In this review, some fundamental considerations and practical approaches are outlined and it is demonstrated how this very sensitive (and distance-dependent) method can be utilized for microarray-based high-throughput screening (HTS) with a focus on protein microarrays. The advantages and also the demands of this dual-label technique in miniaturized multiplexed formats are discussed with respect to its potential readout modes, such as intensity, dual wavelength, and time-resolved FRET detection.
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- Authors
- Schäferling, M., & Nagl, S.
- Affiliations
- University of Regensburg (DE)
- Leipzig University (DE)
- Corresponding author
- Schäferling, Michael
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2011
- Volume
- 723
- Pages
- 303-320
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W2403990523
- Cited by
- 22
- References
- 73
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2015: 2 · 2014: 4 · 2013: 1 · 2012: 5
- FWCI
- 5.81
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- fluorescence resonance energy transfer, protein-protein interaction, protein microarrays, high-throughput screening, time-resolved FRET, dual-wavelength detection
- MeSH terms
- Animals, Humans, Time Factors, Protein Interaction Mapping, Fluorescence Resonance Energy Transfer, Protein Array Analysis
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