Cited by 22Advanced Biosensing Techniques and ApplicationsAdvanced Fluorescence Microscopy TechniquesAdvanced biosensing and bioanalysis techniques

Förster Resonance Energy Transfer Methods for Quantification of Protein–Protein Interactions on Microarrays

Schäferling, Michael, Nagl, Stefan·2011·Methods in Molecular Biology

Abstract

Methods based on Förster (or fluorescence) resonance energy transfer (FRET) are widely used in various areas of bioanalysis and molecular biology, such as fluorescence microscopy, quantitative real-time polymerase chain reaction (PCR), immunoassays, or enzyme activity assays, just to name a few. In the last years, these techniques were successfully implemented to multiplex biomolecular screening on microarrays. In this review, some fundamental considerations and practical approaches are outlined and it is demonstrated how this very sensitive (and distance-dependent) method can be utilized for microarray-based high-throughput screening (HTS) with a focus on protein microarrays. The advantages and also the demands of this dual-label technique in miniaturized multiplexed formats are discussed with respect to its potential readout modes, such as intensity, dual wavelength, and time-resolved FRET detection.

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Crossref · 10.1007/978-1-61779-043-0_19

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Authors
Schäferling, M., & Nagl, S.
Affiliations
  • University of Regensburg (DE)
  • Leipzig University (DE)
Corresponding author
Schäferling, Michael
Venue
Methods in Molecular Biology
Publisher
Humana Press
Published
2011
Volume
723
Pages
303-320
ISSN
  • 1064-3745 (ISSN-L)
  • 1940-6029
OpenAlex
W2403990523
Cited by
22
References
73
Citations by year
2026: 1 · 2024: 1 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2015: 2 · 2014: 4 · 2013: 1 · 2012: 5
FWCI
5.81
Type
book chapter
Language
en
Open access
closed
Locations
  • Methods in molecular biology (publishedVersion)
  • PubMed (publishedVersion)
  • Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
Keywords
fluorescence resonance energy transfer, protein-protein interaction, protein microarrays, high-throughput screening, time-resolved FRET, dual-wavelength detection
MeSH terms
Animals, Humans, Time Factors, Protein Interaction Mapping, Fluorescence Resonance Energy Transfer, Protein Array Analysis

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