The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
Open accessAbstract
The coronavirus disease 2019 (COVID-19) pandemic now has >2,000,000 confirmed cases worldwide. COVID-19 is currently diagnosed using quantitative RT-PCR methods, but the capacity of quantitative RT-PCR methods is limited by their requirement of high-level facilities and instruments. We developed and evaluated reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays to detect genomic RNA of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative virus of COVID-19. RT-LAMP assays reported in this study can detect as low as 100 copies of SARS-CoV-2 RNA. Cross-reactivity of RT-LAMP assays to other human coronaviruses was not observed. A colorimetric detection method was adapted for this RT-LAMP assay to enable higher throughput.
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Cited by
Ortiz-Prado, E., Simbaña-Rivera, K., Gómez- Barreno, L., Rubio-Neira, M., Guaman, L. P., Kyriakidis, N. C., Muslin, C., Jaramillo, A. M. G., Barba-Ostria, C., Cevallos-Robalino, D., et al. (2020) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2020.115094 · Cited by 458
Metadata
- Authors
- Park, G.-S., Ku, K., Baek, S.-H., Kim, S.-J., Kim, S. I., Kim, B.-T., & Maeng, J.-S.
- Venue
- The Journal of Molecular Diagnostics
- Publisher
- Elsevier BV
- Published
- 2020-06
- Volume
- 22
- Issue
- 6
- Pages
- 729-735
- ISSN
- 1525-1578 (ISSN-L)
- OpenAlex
- W3015040045
- Cited by
- 404
- References
- 18
- FWCI
- 10.77
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Park, G.-S., Ku, K., Baek, S.-H., Kim, S.-J., Kim, S. I., Kim, B.-T., & Maeng, J.-S. (2020). Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The Journal of Molecular Diagnostics. https://doi.org/10.1016/j.jmoldx.2020.03.006
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