Monoclonal antibodies discriminating netrin-G1 and netrin-G2 neuronal pathways
Abstract
Netrin-G1 and netrin-G2, belonging to a vertebrate-specific subfamily of the netrin family, distribute on axons of distinct neuronal pathways. To add to the array of molecular probes available for labeling unique neuronal circuits, we generated monoclonal antibodies against the netrin-G1 and netrin-G2 proteins. The monoclonal antibody clones 171A18 and 30B15 differentially labeled specific neuronal circuits, the so-called netrin-G1 or netrin-G2 circuits in mice, respectively. Epitope mapping revealed linear epitopes for these monoclonal antibodies, which are common among splicing variants, and suggested that the anti-netrin-G1 monoclonal antibodies are applicable to various species including humans.
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LII EC axons form excitatory synapses on granule cells in the molecular layer of dentate gyrus, then project onto the distal dendrites of CA3 neurons, and finally terminate in a U-shaped area near the distal dendrites of CA2.
“LII EC axons form excitatory synapses on granule cells in the molecular layer of dentate gyrus (DG), then project onto the distal dendrites of CA3 neurons, and finally terminate in a U shaped-area near the distal dendrites of CA2 (Niimi et al., 2007; Nishimura-Akiyoshi et al., 2007; Tamamaki and Nojyo, 1995).”
From Strong CA2 Pyramidal Neuron Synapses Define a Powerful Disynaptic Cortico-Hippocampal Loop (2010) · cites this paper as Niimi et al., 2007
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NGL family PSD-95–interacting adhesion molecules regulate excitatory synapse formation
Kim, S.-H., Burette, A., Chung, H. S., Kwon, S., Woo, J., Lee, H. W., Kim, K., Kim, H., Weinberg, R. J., & Kim, E. (2006) · Nature Neuroscience · doi:10.1038/nn1763 · Cited by 272 · see citing works
Itohara, S., Nakanishi, N., Kanagawa, O., Kubo, R. T., & Tonegawa, S. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.13.5094 · Cited by 252 · see citing works
Self-citationThe netrin-G1 ligand NGL-1 promotes the outgrowth of thalamocortical axons
Lin, J. C., Ho, W.-H., Gurney, A. L., & Rosenthal, A. (2003) · Nature Neuroscience · doi:10.1038/nn1148 · Cited by 182 · see citing works
Nakashiba, T., Ikeda, T., Nishimura, S., et al. (2000) · Journal of Neuroscience · doi:10.1523/jneurosci.20-17-06540.2000 · Cited by 166 · see citing works
Self-citationComplementary expression and neurite outgrowth activity of netrin-G subfamily members
Nakashiba, T., Nishimura, S., Ikeda, T., et al. (2002) · Mechanisms of Development · doi:10.1016/s0925-4773(01)00600-1 · Cited by 145 · see citing works
Self-citationLane, R. D. (1985) · Journal of Immunological Methods · doi:10.1016/0022-1759(85)90207-8 · Cited by 139 · see citing works
Axonal netrin-Gs transneuronally determine lamina-specific subdendritic segments
Nishimura-Akiyoshi, S., Niimi, K., Nakashiba, T., & Itohara, S. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0706919104 · Cited by 132 · see citing works
Self-citationAoki-Suzuki, M., Yamada, K., Meerabux, J., Iwayama‐Shigeno, Y., Ohba, H., Iwamoto, K., Takao, H., Toyota, T., Suto, Y., Nakatani, N., et al. (2005) · Biological Psychiatry · doi:10.1016/j.biopsych.2004.11.022 · Cited by 105 · see citing works
Self-citationDisruption of Netrin G1 by a balanced chromosome translocation in a girl with Rett syndrome
Borg, I., Freude, K., Kübart, S., Hoffmann, K., Menzel, C., Laccone, F., Firth, H., Ferguson-Smith, M. A., Tommerup, N., Ropers, H.-H., et al. (2005) · European Journal of Human Genetics · doi:10.1038/sj.ejhg.5201429 · Cited by 95 · see citing works
Laminets: Laminin- and Netrin-Related Genes Expressed in Distinct Neuronal Subsets
Yin, Y., Miner, J. H., & Sanes, J. R. (2002) · Molecular and Cellular Neuroscience · doi:10.1006/mcne.2001.1089 · Cited by 84 · see citing works
NTNG1 mutations are a rare cause of Rett syndrome
Archer, H. L., Evans, J., Millar, D. S., Thompson, P., Kerr, A., Leonard, H., Christodoulou, J., Ravine, D., Lazarou, L., Grove, L., et al. (2006) · American Journal of Medical Genetics Part A · doi:10.1002/ajmg.a.31133 · Cited by 52 · see citing works
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Strong CA2 Pyramidal Neuron Synapses Define a Powerful Disynaptic Cortico-Hippocampal Loop
Chevaleyre, V., & Siegelbaum, S. A. (2010) · Neuron · doi:10.1016/j.neuron.2010.04.013 · Cited by 324 · see citing works
- Authors
- Niimi, K., Nishimura-Akiyoshi, S., Nakashiba, T., & Itohara, S.
- Affiliations
- RIKEN Center for Brain Science (JP)
- Massachusetts Institute of Technology (US)
- Corresponding author
- Itohara, Shigeyoshi
- Venue
- Journal of Neuroimmunology
- Publisher
- Elsevier BV
- Published
- 2007-12
- Volume
- 192
- Issue
- 1-2
- Pages
- 99-104
- ISSN
- 0165-5728 (ISSN-L)
- OpenAlex
- W2014748954
- Cited by
- 16
- References
- 11
- Citations by year
- 2023: 1 · 2020: 1 · 2019: 3 · 2014: 2 · 2012: 4
- FWCI
- 0.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroimmunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- RIKEN
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Keywords
- monoclonal antibodies, neural circuits, axon guidance, epitope mapping, splice variants
- MeSH terms
- Netrins, Netrin-1, Animals, Antibodies, Monoclonal, Antibody Specificity, Brain, Nerve Growth Factors, Neurons, Mice, Knockout, Epitope Mapping, Tumor Suppressor Proteins, Mice
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