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Cited by 8Redox biology and oxidative stressAdipose Tissue and MetabolismEffects and risks of endocrine disrupting chemicals

Characterization of the mouse white adipose tissue redox environment and associations with perinatal environmental exposures to bisphenol A and high-fat diets

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Neier, Kari, Marchlewicz, Elizabeth M., Bedrosian, Leah D., Dolinoy, Dana C., Harris, Craig·2019·The Journal of Nutritional Biochemistry
DOI 10.1016/j.jnutbio.2019.01.005PMC PMC7003727
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Abstract

White adipose tissue (WAT) plays an important role in obesity pathophysiology. Redox signaling underlies several aspects of WAT physiology; however, the thiol redox environment of WAT has not yet been fully characterized. Dietary and endocrine disrupting chemical (EDC) exposures during development can transiently impact the cellular redox environment, but it is unknown whether these exposures can reprogram the WAT thiol redox environment. To characterize the WAT thiol redox environment, we took a descriptive approach and measured thiol redox parameters using high-performance liquid chromatography in mouse mesenteric (mWAT), gonadal (gWAT) and subinguinal (sWAT) depots. Cysteine (CYSS:CYS) and glutathione (GSSG:GSH) redox potentials (Eh) were more oxidizing in gWAT and sWAT than mWAT. Increased body weight, relative WAT weight and age were associated with oxidizing GSSG:GSH Eh in mWAT in a sex-specific manner. Body weight and relative WAT weight were also positively associated with GSSG:GSH Eh in sWAT. We carried out a second mouse study with perinatal exposures to bisphenol A (BPA) and Mediterranean and Western high-fat diets (HFDs) to determine whether early-life chemical and dietary factors have long-lasting impacts on mWAT redox parameters. Mice exposed to Mediterranean HFD or BPA had more oxidizing GSSG:GSH mWAT Eh than controls, with more pronounced differences in females. These findings suggest an important role for the thiol redox environment in WAT physiology. Observed sex-specific and depot-specific differences in thiol redox parameters are consistent with known WAT physiology. Lastly, mWAT GSSG:GSH Eh may be reprogrammed by developmental exposure to HFDs and EDCs, which may have implications for obesity risk.

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  • Developmental exposure to BPA is linked to enhanced levels of oxidative stress in the adipose of exposed offspring, and this effect was not prevented by a perinatal Mediterranean diet.

    “Developmental exposure to BPA has also been linked to enhanced levels of oxidative stress in the adipose of exposed offspring however, such an effect was not prevented by exposure to a perinatal Mediterranean diet that is known to offer protection against oxidative stress [114].”

    From Developmental programming of offspring adipose tissue biology and obesity risk (2021) · cites this paper as [114]

    The Results (page not provided) report a more oxidizing adipose redox measure in female offspring exposed to BPA, including those exposed to a perinatal Mediterranean high-fat diet. But the finding was sex-specific, and the Discussion notes that BPA’s effect was not statistically significant after adjustment. The claim overstates this measure as a general increase in adipose oxidative stress among exposed offspring. (LLM, conf=0.84)

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Cited by

Sorted by citations

Authors
Neier, K., Marchlewicz, E. M., Bedrosian, L. D., Dolinoy, D. C., & Harris, C.
Affiliations
University of Michigan (US)
Corresponding author
Harris, Craig
ORCID
Venue
The Journal of Nutritional Biochemistry
Publisher
Elsevier BV
Published
2019-04
Volume
66
Pages
86-97
ISSN
0955-2863 (ISSN-L)
DOI
10.1016/j.jnutbio.2019.01.005
PMC
PMC7003727
OpenAlex
W2910782363
Cited by
8
References
3
Citations by year
2024: 2 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 1
FWCI
0.25
Type
journal article
Language
en
Open access
Licence
Locations
  • The Journal of Nutritional Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • University of Michigan (UM) NIEHS/EPA Children's Environmental Health and Disease Prevention Center (P01 ES022844/RD83543601)
  • Michigan Lifestage Environmental Exposures and Disease (M-LEEaD) NIEHS Core Center (P30 ES017885)
  • UM NIEHS Institutional Training (T32 ES007062, T32 HD079342, F31 ES025101)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
Keywords
white adipose tissue, glutathione redox potential, cysteine, bisphenol A, endocrine disruptors, high-fat diet, perinatal exposure, obesity, sex differences, adipose tissue depots, oxidative stress, developmental programming
MeSH terms
Bisphenol A Compounds, Animals, Benzhydryl Compounds, Body Weight, Chromatography, High Pressure Liquid, Cysteine, Environmental Exposure, Female, Glutathione, Longitudinal Studies, Male, Mice, Inbred C57BL, Obesity, Oxidation-Reduction, Phenols, Pregnancy, Prenatal Exposure Delayed Effects, Diet, Mediterranean, Adiposity, Adipose Tissue, White, Diet, High-Fat, Diet, Western

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