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Diagnosis of Trichomonas vaginalis in female children and adolescents evaluated for possible sexual abuse: a comparison of the InPouch TV culture method and wet mount microscopy.
Abstract
Study objective To compare the performance of wet mount microscopy and the InPouch TV culture method in diagnosing Trichomonas vaginalis in a cohort of female children and adolescents evaluated for possible sexual abuse.Design A prospective study in which samples for both wet mount and InPouch TV were collected from the vaginal vault of each participant. The wet mount was examined microscopically for the presence of motile trichomonads, and the InPouch TV was analyzed in accordance with manufacturer specifications.Setting A regional, university-affiliated sexual abuse center in Nashville, TN.Participants Females (N = 271) aged 10 to 17 years (inclusive) who met screening criteria for sexually transmitted infection testing in accordance with Centers for Disease Control recommendations, and who exhibited evidence of at least partial hymenal estrogenization.Main outcome measures A Trichomonas vaginalis diagnosis either via detection of motile trichomonads on wet mount or a positive InPouch TV.Results The median age of the 271 participants was 13.6 years, 88% (235/268) were genital Tanner stage 4 or 5, and 66% (173/263) were Caucasian. Trichomonas vaginalis was diagnosed by either wet mount or InPouch TV in 4% (12/274) of the possible sexually transmitted infection exposures. The sensitivity of wet mount was 8/12 = 0.67 (95% confidence interval [CI]: 0.39, 0.86) and specificity was 262/262 = 1.00 (95% CI: 0.99, 1.00). The false negative rate of wet mount was 4/12 = 0.33 (95% CI: 0.14, 0.61).Conclusions A culture-based method such as InPouch TV should be considered for Trichomonas vaginalis diagnosis when evaluating children and adolescents in whom sexual abuse is a concern.
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“Testing for T. vaginalis should not be limited to girls with vaginal discharge if other indications for vaginal testing exist because evidence indicates that asymptomatic sexually abused children might be infected with T. vaginalis and might benefit from treatment (1451,1452).”
From Sexually Transmitted Infections Treatment Guidelines, 2021 (2021) · cites this paper as [1452]
Citations
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A case-control study of anatomic changes resulting from sexual abuse
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Sexually Transmitted Diseases in Victims of Rape
Jenny, C., Hooton, T. M., Bowers, A., Copass, M. K., Krieger, J. N., Hillier, S. L., Kiviat, N. B., Corey, L., Stamm, W. E., & Holmes, K. K. (1990) · New England Journal of Medicine · doi:10.1056/nejm199003153221101 · Cited by 192
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Sexually Transmitted Infections Treatment Guidelines, 2021
Workowski, K. A., Bachmann, L. H., Chan, P. A., Johnston, C. M., Muzny, C. A., Park, I., Reno, H., Zenilman, J. M., & Bolan, G. A. (2021) · MMWR. Recommendations and Reports · doi:10.15585/mmwr.rr7004a1 · Cited by 3,018
Metadata
- Authors
- Gallion, H. R., Dupree, L. J., Scott, T. A., & Arnold, D. H.
- Affiliations
- Meharry Medical College (US)
- Vanderbilt University (US)
- Corresponding author
- Gallion, Hollye R.
- Venue
- Journal of Pediatric and Adolescent Gynecology
- Publisher
- Elsevier BV
- Published
- 2009-10
- Volume
- 22
- Issue
- 5
- Pages
- 300-305
- ISSN
- 0932-8610 (ISSN-L)
- 1083-3188
- OpenAlex
- W2093686297
- Cited by
- 25
- References
- 26
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 1 · 2012: 3
- FWCI
- 2.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pediatric and Adolescent Gynecology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Adolescent Sexual and Reproductive Health
- Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
- Keywords
- Trichomonas vaginalis, trichomoniasis diagnosis, wet mount microscopy, sexually transmitted infections, child sexual abuse, adolescent girls, sensitivity and specificity
- MeSH terms
- Adolescent, Bacteriological Techniques, Child, Child Abuse, Sexual, Female, Humans, Prospective Studies, Sensitivity and Specificity, Trichomonas Vaginitis, Logistic Models
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- Gallion, H. R., Dupree, L. J., Scott, T. A., & Arnold, D. H. (2009). Diagnosis of Trichomonas vaginalis in female children and adolescents evaluated for possible sexual abuse: a comparison of the InPouch TV culture method and wet mount microscopy. Journal of Pediatric and Adolescent Gynecology. https://doi.org/10.1016/j.jpag.2008.12.006