Development of an LC-MS/MS method for quantitative analysis of Chlorogenic acid in human plasma and its application to a pharmacokinetic study in Chinese patients with advanced solid tumor
Abstract
A simple and specific, rapid resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for determination of chlorogenic acid in human plasma using neochlorogenic acid as the internal standard. Plasma samples were precipitated with methanol and separated on a Zorbax C18 column (50 × 2.1 mm, i.d. 1.8 μm) at a flow rate of 0.4 mL/min using a gradient mobile phase of methanol-water containing 0.1% formic acid (v/v). The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring in negative ESI mode. The method was fully validated over the concentration range of 10-2000 ng/mL. The indicators of inter- and intra-day precision (RSD%) were all within 10.7%, and the accuracy (RE%) was ranged from -3.0% to 10.6%. Moreover, we evaluated this bioanalytical method by re-analysis of incurred samples as an additional measure of assay reproducibility. This method was successfully applied to pharmacokinetic study of CGA in Chinese subjects with advanced solid tumor after intramuscular injection administration of Chlorogenic acid for injection (CAFI).
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Yuan, Q., Liu, C., Zhang, Z., Chen, F., Xiao, Q., Zhang, L., Pan, X., He, F., & Xiao, M. (2025) · Frontiers in Pharmacology · doi:10.3389/fphar.2025.1613048 · Cited by 27 · see citing works
- Authors
- Yang, F., Gong, J. F., Shen, L., Zhang, C., Kou, F. R., Gao, J., Li, Y., & bing Xu, G.
- Affiliations
- Peking University Cancer Hospital (CN)
- Corresponding author
- Gong, Ji fang
- Venue
- Journal of Pharmaceutical and Biomedical Analysis
- Publisher
- Elsevier BV
- Published
- 2020-01
- Volume
- 177
- Pages
- 112809
- ISSN
- 0731-7085 (ISSN-L)
- OpenAlex
- W2968387255
- Cited by
- 25
- References
- 0
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 4 · 2023: 3 · 2022: 2 · 2021: 4 · 2020: 1
- FWCI
- 0.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmaceutical and Biomedical Analysis (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- National Major Science and Technology Projects of China
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Life Sciences › Immunology and Microbiology › Immunology › Biosimilars and Bioanalytical Methods
- Keywords
- chlorogenic acid, pharmacokinetics, human plasma, LC-MS/MS method development, advanced solid tumors, intramuscular injection, Chinese patients, bioanalytical method validation
- MeSH terms
- China, Chlorogenic Acid, Chromatography, High Pressure Liquid, Dose-Response Relationship, Drug, Drug Administration Schedule, Drug Stability, Humans, Injections, Intramuscular, Neoplasms, Quinic Acid, Sensitivity and Specificity, Reproducibility of Results, Anticarcinogenic Agents, Area Under Curve, Tandem Mass Spectrometry, High-Throughput Screening Assays
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