Simultaneous determination of three curcuminoids in Curcuma longa L. by high performance liquid chromatography coupled with electrochemical detection
Open accessAbstract
A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column and detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4–H3PO4 (pH 5.0) (50:50, v/v) was used. Good linearity was obtained in the range of 0.208–41.6, 0.197–39.4, and 0.227–114 μM for curcumin, demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10−8 M, which was lower than that by UV detection. The relative standard deviations (RSDs) ranged from 1.06% to 1.88% for intra-day precision and from 4.30% to 5.79% for inter-day precision, respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3% to 111% were obtained.
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Several natural products, mainly plant extracts, have been reported to be used in traditional medicine for neuroprotective, memory enhancing, and antiageing functions.
“Several natural products, mainly plants extracts, have been reported to be used in traditional medicine for neuroprotective, memory enhancing, and antiageing functions”
From Multi-target neuroprotective effects of herbal medicines for Alzheimer's disease (2022) · cites this paper as Long et al., 2014
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Multi-target neuroprotective effects of herbal medicines for Alzheimer's disease
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- Authors
- Long, Y., Zhang, W., Wang, F., & Chen, Z.
- Affiliations
- Wuhan University (CN)
- Corresponding author
- Chen, Zilin
- Venue
- Journal of Pharmaceutical Analysis
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 4
- Issue
- 5
- Pages
- 325-330
- ISSN
- 2095-1779 (ISSN-L)
- PMC
- PMC5761211
- OpenAlex
- W2024826746
- Cited by
- 53
- References
- 34
- Citations by year
- 2025: 6 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 8 · 2019: 8 · 2018: 6 · 2017: 5 · 2016: 5 · 2015: 5 · 2014: 1
- FWCI
- 1.69
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Pharmaceutical Analysis (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ministry of Education of the People's Republic of China (20110141110024)
- National Natural Science Foundation of China (21375101, 30973672, 90817103)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Curcumin's Biomedical Applications
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Keywords
- curcumin, demethoxycurcumin, bisdemethoxycurcumin, curcuminoids, turmeric, simultaneous determination, electrochemical detection, HPLC-ED
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