Expression of sweet potato cysteine protease SPCP2 altered developmental characteristics and stress responses in transgenic Arabidopsis plants
Abstract
In this report a full-length cDNA, SPCP2, which encoded a putative papain-like cysteine protease was isolated from senescent leaves of sweet potato (Ipomoea batatas). SPCP2 contained 1101 nucleotides (366 amino acids) in its open reading frame, and exhibited high amino acid sequence identities (ca. 68% to 83%) with plant cysteine proteases, including Actinidia deliciosa, Arabidopsis thaliana, Brassica oleracea, Phaseolus vulgaris, Pisum sativa, Vicia faba, Vicia sativa and Vigna mungo. RT-PCR analysis showed that SPCP2 gene expression was enhanced significantly in natural senescent leaves and in dark-, abscisic acid- (ABA-), jasmonic acid- (JA-) and ethephon-induced senescent leaves, but was almost not detected in mature green leaves, stems, and roots. Transgenic Arabidopsis with constitutive SPCP2 expression exhibited earlier floral transition from vegetative to reproductive growth, higher percentage of incompletely developed siliques per plant, reduced average fresh weight and lower germination percentage of seed, and higher salt and drought stress tolerance compared to those of control. Based on these results we conclude that sweet potato papain-like cysteine protease, SPCP2, is a functional senescence-associated gene, and its expression causes altered developmental characteristics and stress responses in transgenic Arabidopsis plants.
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Sporamin and β-amylase account for about 60% and 5% of total soluble proteins in sweetpotato storage roots, respectively.
“with sporamin and β-amylase accounting for about 60% and 5% of total soluble proteins in sweetpotato SRs, respectively [28,29].”
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- Authors
- Chen, H.-J., Su, C.-T., Lin, C.-H., Huang, G.-J., & Lin, Y.-H.
- Affiliations
- National Sun Yat-sen University (TW)
- Chinese Culture University (TW)
- China Medical University (TW)
- Institute of Plant and Microbial Biology, Academia Sinica (TW)
- Corresponding author
- Chen, Hsien-Jung
- Lin, Yaw-Huei
- Venue
- Journal of Plant Physiology
- Publisher
- Elsevier BV
- Published
- 2010-07
- Volume
- 167
- Issue
- 10
- Pages
- 838-847
- ISSN
- 0176-1617 (ISSN-L)
- OpenAlex
- W2039545369
- Cited by
- 102
- References
- 42
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 10 · 2023: 11 · 2022: 9 · 2021: 7 · 2020: 2 · 2019: 1 · 2018: 6 · 2017: 5 · 2016: 2 · 2015: 5 · 2014: 7 · 2013: 11 · 2012: 8
- FWCI
- 9.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Plant Physiology (publishedVersion)
- PubMed (publishedVersion)
- Chinese Culture University Repository (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Keywords
- papain-like cysteine protease, Ipomoea batatas, leaf senescence, transgenic Arabidopsis, floral induction, seed germination, salt tolerance, drought tolerance, abscisic acid, jasmonic acid, ethephon
- MeSH terms
- Amino Acid Sequence, Base Sequence, Molecular Sequence Data, Stress, Physiological, Gene Expression, Genes, Plant, Arabidopsis, DNA Primers, DNA, Plant, Ipomoea batatas, Plants, Genetically Modified, Salinity, Droughts, Cysteine Proteases
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