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Semi-automated selection of cryo-EM particles in RELION-1.3

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Scheres, Sjors H.W.·2015·Journal of Structural Biology
DOI 10.1016/j.jsb.2014.11.010
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Abstract

The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives. With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection. Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach.

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Research works printed but not linked: 39

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Metadata

Authors
Scheres, S. H. W.
Venue
Journal of Structural Biology
Publisher
Elsevier BV
Published
2015-02
Volume
189
Issue
2
Pages
114-122
ISSN
1047-8477 (ISSN-L)
DOI
10.1016/j.jsb.2014.11.010
OpenAlex
W1982295411
Cited by
377
References
0
FWCI
18.60
Type
journal article
Language
en
Open access
hybrid
Licence
Funders
UK Medical Research Council (MC_UP_A025_1013)

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CoChat. (2026). Citation check: Semi-automated selection of cryo-EM particles in RELION-1.3. https://app.cochat.ai/papers/10.1016/j.jsb.2014.11.010
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Scheres, S. H. W. (2015). Semi-automated selection of cryo-EM particles in RELION-1.3. Journal of Structural Biology. https://doi.org/10.1016/j.jsb.2014.11.010

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