The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Rapid quantitation of porcine epidemic diarrhea virus (PEDV) by Virus Counter
Abstract
Virus Counter was applied for the quantitation of porcine epidemic diarrhea virus (PEDV); the working range and reproducibility of the quantification were characterized, and the performance of Virus Counter was evaluated using the plaque assay as the gold standard. In this study, the single burst curves of PEDV were first constructed with the Virus Counter or plaque assay respectively, and the results from Virus Counter and plaque assay showed similar high correlation for interval of 12-24 h (r=0.9581, p<0.05). Then three virus batches from independent virus propagations were harvested at interval of 16-20 h and serial-diluted; The correlation between the calculated titer and the results obtained by Virus Counter were better than those obtained by using the plaque assay. The comparison between the plaque assay data and the Virus Counter results revealed a linear relationship (slope=1.155±0.46, R(2)=0.9665) with a significant Pearson correlation (r=0.9809, p<0.0001). The results of this study demonstrated that Virus Counter is a simple and reliable method to quantify the viral particles of PEDV.
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FV repeatability for rAAV2 and -3 is around 5% CV but commonly 10%-30% CV in independent studies on other viruses.
“Repeatability for rAAV2 and -3 is reported around 5% CV but commonly 10%–30% CV in independent studies on other viruses.”
From Analytical methods for process and product characterization of recombinant adeno-associated virus-based gene therapies (2021) · cites this paper as [66]
Citations
Cites
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Gimpel, A. L., Katsikis, G., Sha, S., Maloney, A. J., Hong, M. S., Nguyen, T. N. T., Wolfrum, J., Springs, S. L., Sinskey, A. J., Manalis, S. R., et al. (2021) · Molecular Therapy - Methods & Clinical Development · doi:10.1016/j.omtm.2021.02.010 · Cited by 185
Metadata
- Authors
- Zhang, X., Hao, J., Zhen, J., Yin, L., Li, Q., Xue, C., & Cao, Y.
- Affiliations
- Sun Yat-sen University (CN)
- Corresponding author
- Cao, Yongchang
- Venue
- Journal of Virological Methods
- Publisher
- Elsevier BV
- Published
- 2015-10
- Volume
- 223
- Pages
- 1-4
- ISSN
- 0166-0934 (ISSN-L)
- OpenAlex
- W757245412
- Cited by
- 9
- References
- 14
- Citations by year
- 2026: 1 · 2024: 1 · 2021: 1 · 2018: 4 · 2017: 1 · 2016: 1
- FWCI
- 2.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Virological Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Disease Management and Epidemiology
- Keywords
- porcine epidemic diarrhea virus, virus quantitation, plaque assay, viral titer, viral particle release, rapid virus detection
- MeSH terms
- Animals, Time Factors, Reproducibility of Results, Viral Load, Porcine epidemic diarrhea virus
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