Membrane protein hMYADM preferentially expressed in myeloid cells is up-regulated during differentiation of stem cells and myeloid leukemia cells
Abstract
We report here the molecular cloning and characterization of a novel human gene (hMYADM) derived from a human bone marrow stromal cell (BMSC) cDNA library, which shares high homology with mouse myeloid-associated differentiation marker (MYADM). hMYADM is also closely related to many other eukaryotic proteins, which together form a novel and highly conserved MYADM-like family. hMYADM with 322-residue protein contains eight putative transmembrane segments and confocal microscopic analysis confirmed its membrane localization by using anti-hMYADM monoclonal antibody. hMYADM mRNA was selectively expressed in human monocytes, dendritic cells, promyeloid or monocytic leukemia cell lines, but not in CD4+, CD8+, CD19+ cells, nor in T cell leukemia or lymphocytic leukemia cell lines. hMYADM expression was also found in normal human bone marrow enriched for CD34+ stem cells, and the expression was up-regulated when these cells were induced to differentiate toward myeloid cells. The mRNA expression level of hMYADM significantly increased in acute promyelocytic leukemia HL-60 and chronic myelogenous leukemia K562 cell line after phorbol myristate acetate (PMA)-induced differentiation. Our study suggests that hMYADM is selectively expressed in myeloid cells, and involved in the myeloid differentiation process, indicating that hMYADM may be one useful membrane marker to monitor stem cell differentiation or myeloid leukemia differentiation.
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Multiple genes expressed in differentiated myeloid cells, including CSF2RA, ITGAM (Mac1), CEBPE and ELANE, were significantly upregulated (FDR<0.05) upon vitC treatment.
“Further examination revealed significant upregulation (false discovery rate <0.05) of multiple genes expressed in differentiated myeloid cells, including CSF2RA,23 ITGAM (Mac1), CEBPE24 and ELANE,25 and increased expression of genes encoding cell surface markers that define mature myeloid cells (Supplementary Figure S2b, upper).”
From Vitamin C-induced epigenomic remodelling in IDH1 mutant acute myeloid leukaemia (2018) · cites this paper as [25]
Cites
2 retracted
Wang, X., Li, N., Liu, B., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m405147200 · Cited by 93 · see citing works
Self-citationRetractedYang, L., Li, N., Wang, C., Yu, Y., Yuan, L., Zhang, M., & Cao, X. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m312895200 · Cited by 88 · see citing works
Self-citationRetractedMicroRNAs Modulate Hematopoietic Lineage Differentiation
Chen, C.-Z., Li, L., Lodish, H. F., & Bartel, D. P. (2004) · Science · doi:10.1126/science.1091903 · Cited by 3,257 · see citing works
Identification of Flt3+ Lympho-Myeloid Stem Cells Lacking Erythro-Megakaryocytic Potential
Adolfsson, J., Månsson, R., Buza-Vidas, N., Hultquist, A., Liuba, K., Jensen, C. T., Bryder, D., Yang, L., Borge, O. J., Thorén, L., et al. (2005) · Cell · doi:10.1016/j.cell.2005.02.013 · Cited by 1,229 · see citing works
Developmental potential and dynamic behavior of hematopoietic stem cells
Lemischka, I. R., Raulet, D. H., & Mulligan, R. C. (1986) · Cell · doi:10.1016/0092-8674(86)90566-0 · Cited by 880 · see citing works
Prospective isolation of human clonogenic common myeloid progenitors
Manz, M. G., Miyamoto, T., Akashi, K., & Weissman, I. L. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.172384399 · Cited by 529 · see citing works
Extensive Amplification and Self-Renewal of Human Primitive Hematopoietic Stem Cells From Cord Blood
Piacibello, W., Sanavio, F., Garetto, L., Severino, A., Bergandi, D., Ferrario, J., Fagioli, F., Berger, M., & Aglietta, M. (1997) · Blood · doi:10.1182/blood.v89.8.2644 · Cited by 431 · see citing works
MARVEL: a conserved domain involved in membrane apposition events
Sánchez‐Pulido, L., Martín‐Belmonte, F., Valencia, A., & Alonso, M. A. (2002) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(02)02229-6 · Cited by 236 · see citing works
Salmon, P., Kindler, V., Ducrey, O., Chapuis, B., Zubler, R. H., & Trono, D. (2000) · Blood · doi:10.1182/blood.v96.10.3392 · Cited by 232 · see citing works
Ye, M., Iwasaki, H., Laiosa, C. V., Stadtfeld, M., Xie, H., Heck, S., Clausen, B. E., Akashi, K., & Graf, T. (2003) · Immunity · doi:10.1016/s1074-7613(03)00299-1 · Cited by 194 · see citing works
Kato, Y., Iwama, A., Tadokoro, Y., Shimoda, K., Minoguchi, M., Akira, S., Tanaka, M., Miyajima, A., Kitamura, T., & Nakauchi, H. (2005) · The Journal of Experimental Medicine · doi:10.1084/jem.20042541 · Cited by 178 · see citing works
Richards, M. K., Liu, F., Iwasaki, H., Akashi, K., & Link, D. C. (2003) · Blood · doi:10.1182/blood-2003-02-0593 · Cited by 177 · see citing works
Liu, S., Yu, Y., Zhang, M., Wang, W., & Cao, X. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.9.5407 · Cited by 165 · see citing works
Self-citationLi, N., Zhang, W., Wan, T. Y., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m100467200 · Cited by 93 · see citing works
Self-citationRegulatory events in early and late B-cell differentiation
Johnson, K., Shelef, M. A., Tunyaplin, C., & Calame, K. L. (2005) · Molecular Immunology · doi:10.1016/j.molimm.2004.06.039 · Cited by 64 · see citing works
Genetic networks that regulate B lymphopoiesis
Medina, K. L., & Singh, H. (2005) · Current Opinion in Hematology · doi:10.1097/01.moh.0000160735.67596.a0 · Cited by 58 · see citing works
Chen, T., Han, Y., Yang, M., et al. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(03)00482-0 · Cited by 50 · see citing works
Molecular pathways regulating the self-renewal of hematopoietic stem cells
Stein, M. I., Zhu, J., & Emerson, S. G. (2004) · Experimental Hematology · doi:10.1016/j.exphem.2004.08.012 · Cited by 45 · see citing works
Manfredini, R., Zini, R., Salati, S., et al. (2005) · Stem Cells · doi:10.1634/stemcells.2004-0265 · Cited by 45 · see citing works
Smad7 alters cell fate decisions of human hematopoietic repopulating cells
Chadwick, K., Shojaei, F., Gallacher, L. M., et al. (2004) · Blood · doi:10.1182/blood-2004-03-0881 · Cited by 42 · see citing works
Hatta, M., Nagai, H., Okino, K., et al. (2004) · Journal of obstetrics and gynaecology research · doi:10.1111/j.1341-8076.2004.00156.x · Cited by 38 · see citing works
The Complex Cartography of Stem Cell Commitment
Akashi, K., Traver, D. J., & Zon, L. I. (2005) · Cell · doi:10.1016/j.cell.2005.04.005 · Cited by 37 · see citing works
Pettersson, M., Dannaeus, K., Nilsson, K., et al. (2000) · Journal of Leukocyte Biology · doi:10.1002/jlb.67.3.423 · Cited by 33 · see citing works
Marazuela, M., Acevedo, A., García-López, M. Á., et al. (2004) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215540405200212 · Cited by 27 · see citing works
Hamacher, M., Pippirs, U., Köhler, A., et al. (2001) · Mammalian Genome · doi:10.1007/s00335-001-3035-5 · Cited by 22 · see citing works
Shuxun, L., Yu, Y., An, H., et al. (2003) · Immunology Letters · doi:10.1016/s0165-2478(03)00004-x · Cited by 16 · see citing works
Cui, W., Yu, L., He, H., Chu, Y., Gao, J., Wan, B., Tang, L., & Zhao, S. (2001) · Molecular Biology Reports · doi:10.1023/a:1015288412047 · Cited by 14 · see citing works
Genesis of Hematopoietic Stem Cells In Vitro and In Vivo: New Insights into Developmental Maturation
Kyba, M. (2005) · International Journal of Hematology · doi:10.1532/ijh97.04192 · Cited by 8 · see citing works
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Vitamin C-induced epigenomic remodelling in IDH1 mutant acute myeloid leukaemia
Mingay, M., Chaturvedi, A., Bilenky, M., Cao, Q., Jackson, L., Hui, T., Moksa, M., Heravi-Moussavi, A., Humphries, R. K., Heuser, M., et al. (2018) · Leukemia · doi:10.1038/leu.2017.171 · Cited by 94 · see citing works
- Authors
- Wang, Q., Li, N., Wang, X., Shen, J., Hong, X., Yu, H., Zhang, Y., Wan, T., Zhang, L., Wang, J., & Cao, X.
- Affiliations
- Zhejiang University (CN)
- Second Military Medical University (CN)
- Corresponding author
- Cao, Xuetao
- Venue
- Life Sciences
- Publisher
- Elsevier BV
- Published
- 2007-01
- Volume
- 80
- Issue
- 5
- Pages
- 420-429
- ISSN
- 0024-3205 (ISSN-L)
- OpenAlex
- W2032058353
- Cited by
- 17
- References
- 28
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 3 · 2017: 2 · 2016: 1 · 2015: 1
- FWCI
- 0.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Life Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Zhejiang Province
- Topics
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- membrane proteins, myeloid differentiation, myeloid leukemia, mesenchymal stem cells, CD34+ cells, monocytes, dendritic cells, acute promyelocytic leukemia, chronic myeloid leukemia, PMA-induced differentiation, cell surface markers
- MeSH terms
- Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigens, Differentiation, Cattle, Cell Differentiation, Cell Membrane, Cloning, Molecular, Dogs, Hematopoietic Stem Cells, Humans, Leukemia, Myeloid, Molecular Sequence Data, Proteins, Transfection, Protein Biosynthesis, Blotting, Northern, Blotting, Western, Up-Regulation, Sequence Alignment, Sequence Homology, Cell Line, Tumor, Mice, Rats, Myelin and Lymphocyte-Associated Proteolipid Proteins
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