The role of nicotinic receptors in B-lymphocyte development and activation
Abstract
We studied the binding of [(3)H]-epibatidine and [(125)I-]alpha-bungarotoxin, as well as subunit-specific antibodies with purified B lymphocytes of C57Bl/6J mice and found that these cells contained 12,200+/-3200 of alpha4(alpha5)beta2 and 3130+/-750 of alpha7(alpha5beta4) nicotinic acetylcholine receptors per cell. According to flow cytometry data, the highest expression of alpha4(alpha5)beta2 receptors was observed in immature newly generated B lymphocytes of the bone marrow, while the number of alpha7(alpha5beta4) receptors grew up along with the B cell maturation in the spleen. By using alpha4, beta2 or alpha7 knockout and chimera mice, it was shown that both receptor subtypes supported the survival of B cell precursors and increased the size of B-lymphocyte population in the bone marrow. In contrast, propagation of mature B lymphocytes in the spleen was controlled by alpha7-containing subtype only. Moreover, mature B lymphocytes became sensitive to nicotine only in the absence of beta2-containing receptors. Knockout mice had less serum IgG, IgG-producing cells and natural IgG antibodies than their wild-type counterparts, while the absence of beta2-containing receptors resulted in increased B-lymphocyte activation and antibody immune response. The data obtained indicate that nicotinic receptors are involved in regulating B-lymphocyte development and activation, possibly, by affecting expression and/or signaling of CD40, the two subtypes playing different roles.
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SLURP1 is expressed in the periphery, not in neurons, is not GPI anchored, but is secreted out of the cell.
“SLURP1 differs from lynx1 in that SLURP1 is expressed in the periphery, not in neurons, and is not GPI anchored, but is secreted out of the cell (121,122).”
From Nicotine is a selective pharmacological chaperone of acetylcholine receptor number and stoichiometry. Implications for drug discovery (2009) · cites this paper as [122]
Cites
Nicotinic acetylcholine receptor α7 subunit is an essential regulator of inflammation
Wang, H., Yu, M., Ochani, M., Amella, C. A., Tanovic, M., Susarla, S., Li, J. H., Wang, H., Yang, H., Ulloa, L., et al. (2003) · Nature · doi:10.1038/nature01339 · Cited by 3,228 · see citing works
Neuronal nicotinic receptors in the human brain
Paterson, D., & Nordberg, A. K. (2000) · Progress in Neurobiology · doi:10.1016/s0301-0082(99)00045-3 · Cited by 782 · see citing works
Gershwin, E. M., Selmi, C., Worman, H. J., Gold, E. B., Watnik, M., Utts, J., Lindor, K. D., Kaplan, M. M., & Vierling, J. M. (2005) · Hepatology · doi:10.1002/hep.20907 · Cited by 621 · see citing works
Extraneuronal cholinergic system in lymphocytes
Kawashima, K. (2000) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(99)00071-6 · Cited by 384 · see citing works
Biological Functions of Keratinocyte Cholinergic Receptors
Grando, S. A. (1997) · Journal of Investigative Dermatology Symposium Proceedings · doi:10.1038/jidsymp.1997.10 · Cited by 243 · see citing works
Nicotine administration to rats: Methodological considerations
Murrin, L. C., Ferrer, J. R., Zeng, W., & Haley, N. J. (1987) · Life Sciences · doi:10.1016/0024-3205(87)90020-8 · Cited by 237 · see citing works
Neuronal nicotinic receptors in non-neuronal cells: new mediators of tobacco toxicity?
Conti‐Fine, B. M., Navaneetham, D., Lei, S., & Maus, A. D. J. (2000) · European Journal of Pharmacology · doi:10.1016/s0014-2999(00)00036-4 · Cited by 173 · see citing works
Bellinger, D. L., Lorton, D., Hamill, R. W., Felten, S. Y., & Felten, D. L. (1993) · Brain Behavior and Immunity · doi:10.1006/brbi.1993.1021 · Cited by 148 · see citing works
Risk factors for functional limitations in patients with long‐standing ankylosing spondylitis
Ward, M. M., Weisman, M. H., Davis, J. C., et al. (2005) · Arthritis Care & Research · doi:10.1002/art.21444 · Cited by 138 · see citing works
Effects of nicotine on the threshold for rewarding brain stimulation in rats
Huston-Lyons, D., & Kornetsky, C. H. (1992) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(92)90223-3 · Cited by 107 · see citing works
Nicotinic receptors regulate B lymphocyte activation and immune response
Skok, M., Grailhe, R., & Changeux, J.-P. (2005) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2005.05.011 · Cited by 88 · see citing works
Self-citationNoradrenergic and cholinergic innervation of the bone marrow
Artico, M., Bosco, S., Cavallotti, C. A. P., Agostinelli, E., Giuliani-Piccari, G., Sciorio, S., Cocco, L., & Vitale, M. (2002) · International Journal of Molecular Medicine · doi:10.3892/ijmm.10.1.77 · Cited by 85 · see citing works
Functional Nicotinic Acetylcholine Receptors Are Expressed in B Lymphocyte-Derived Cell Lines
Skok, M. V., Kalashnik, E. N., Koval, L. N., Tsetlin, V. I., Utkin, Y. N., Changeux, J., & Grailhe, R. (2003) · Molecular Pharmacology · doi:10.1124/mol.64.4.885 · Cited by 85 · see citing works
Self-citationThe role of nicotinic acetylcholine receptors in lymphocyte development
Skok, M., Grailhe, R., Agenès, F., & Changeux, J. (2005) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2005.09.011 · Cited by 71 · see citing works
Self-citationCigarette smoking and systemic lupus erythematosus: A smoking gun?
Costenbader, K. H., & Karlson, E. W. (2005) · Autoimmunity · doi:10.1080/08916930500285758 · Cited by 54 · see citing works
Molecular and Antigenic Structure of Nicotinic Acetylcholine Receptors
Lindstrom, J. M., Peng, X.-D., Kuryatov, A., Lee, E. B., Anand, R., Gerzanich, V., WANG, F., Wells, G. B., & NELSON, M. (1998) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1998.tb10910.x · Cited by 36 · see citing works
Loembé, M. M., Lamoureux, J., Deslauriers, N., et al. (2001) · British Journal of Haematology · doi:10.1046/j.1365-2141.2001.02806.x · Cited by 21 · see citing works
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Singh, M. V., Chapleau, M. W., Harwani, S. C., & Abboud, F. M. (2014) · Immunologic Research · doi:10.1007/s12026-014-8548-6 · Cited by 176 · see citing works
Lester, H. A., Xiao, C., Srinivasan, R., Son, C. D., Miwa, J., Pantoja, R., Banghart, M. R., Dougherty, D. A., Goate, A. M., & Wang, J. C. (2009) · The AAPS Journal · doi:10.1208/s12248-009-9090-7 · Cited by 163 · see citing works
B cells and autoantibodies: Complex roles in CNS injury
Ankeny, D. P., & Popovich, P. G. (2010) · Trends in Immunology · doi:10.1016/j.it.2010.06.006 · Cited by 116 · see citing works
- Authors
- Skok, M. V., Grailhe, R., Agenes, F., & Changeux, J.-P.
- Affiliations
- Palladin Institute of Biochemistry (UA)
- Institut Pasteur Korea (KR)
- Institut Pasteur (FR)
- Corresponding author
- Skok, M.V.
- Venue
- Life Sciences
- Publisher
- Elsevier BV
- Published
- 2007-05
- Volume
- 80
- Issue
- 24-25
- Pages
- 2334-2336
- ISSN
- 0024-3205 (ISSN-L)
- OpenAlex
- W2049866461
- Cited by
- 100
- References
- 17
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 2 · 2023: 3 · 2022: 6 · 2021: 8 · 2020: 4 · 2019: 6 · 2018: 4 · 2017: 6 · 2016: 9 · 2015: 3 · 2014: 4 · 2013: 3 · 2012: 6
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Life Sciences (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Neuroscience › Neurology › Vagus Nerve Stimulation Research
- Keywords
- nicotinic acetylcholine receptors, B cell development, B cell activation, alpha7 nicotinic acetylcholine receptor, nicotine, knockout mice, chimeric mice, B cell precursors, B cell maturation, antibody response, CD40 signaling, epibatidine binding, alpha-bungarotoxin binding
- MeSH terms
- Animals, Antibodies, B-Lymphocytes, Binding, Competitive, Lymphocyte Activation, Receptors, Nicotinic, Protein Subunits, Mice
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