GnRH agonist reduces estrogen receptor dimerization in GT1-7 cells: Evidence for cross-talk between membrane-initiated estrogen and GnRH signaling
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17β-estradiol (E2), a key participant on the initiation of the LH surge, exerts both positive and negative feedback on GnRH neurons. We sought to investigate potential interactions between estrogen receptors alpha (ERα) and beta (ERβ) and gonadotropin releasing hormone receptor (GnRH-R) in GT1-7 cells. Radioligand binding studies demonstrated a significant decrease in saturation E2 binding in cells treated with GnRH agonist. Conversely, there was a significant reduction in GnRH binding in GT1-7 cells treated with E2. In BRET(1) experiments, ERα-ERα dimerization was suppressed in GT1-7 cells treated with GnRH agonist (p < 0.05). There was no evidence of direct interaction between ERs and GnRH-R. This study provides the first evidence of reduced ERα homodimerization by GnRH agonist. Collectively, these findings demonstrate significant cross-talk between membrane-initiated GnRH and E2 signaling in GT1-7 cells.
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GnRH and GnRHR have been detected in the placenta and decidua of several species.
“as GnRH and GnRHR have been detected in the placenta and decidua of several species23–28.”
From Conditional loss of ERK1 and ERK2 results in abnormal placentation and delayed parturition in the mouse (2019) · cites this paper as [26]
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- Authors
- Chason, R. J., Kang, J.-H., Gerkowicz, S. A., Dufau, M. L., Catt, K. J., & Segars, J. H.
- Affiliations
- National Institutes of Health (US)
- University of Miami (US)
- Corresponding author
- Segars, James H.
- Venue
- Molecular and Cellular Endocrinology
- Publisher
- Elsevier BV
- Published
- 2015-03
- Volume
- 404
- Pages
- 67-74
- ISSN
- 0303-7207 (ISSN-L)
- PMC
- PMC4590284
- OpenAlex
- W1965653369
- Cited by
- 14
- References
- 53
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 2 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (Z01-HD 008737, Z01-HD 00184, ZIA-HD 000150-39)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- GnRH agonist, estrogen receptor alpha, estrogen receptor beta, GnRH receptor, GnRH signaling, crosstalk, GT1-7 cells, estradiol, GnRH neurons, luteinizing hormone surge, negative feedback
- MeSH terms
- Binding Sites, Cell Line, Estradiol, Humans, Gonadotropin-Releasing Hormone, Neurons, Radioligand Assay, Signal Transduction, Estrogen Receptor alpha, Estrogen Receptor beta, Protein Multimerization, HEK293 Cells, Bioluminescence Resonance Energy Transfer Techniques
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