CoChat
Sign Up
Cited by 74Bacterial Identification and Susceptibility TestingOral microbiology and periodontitis researchActinomycetales infections and treatment

Isolation and identification methods of Rothia species in oral cavities

Tsuzukibashi, Osamu, Uchibori, Satoshi, Kobayashi, Taira, Umezawa, Koji, Mashimo, Chiho, Nambu, Takayuki, Saito, Masanori, Hashizume-Takizawa, Tomomi +1 more·2017·Journal of Microbiological Methods
DOI 10.1016/j.mimet.2017.01.005
Open this paper in CoChat — free account

Abstract

Rothia dentocariosa and Rothia mucilaginosa which are Gram-positive bacteria are part of the normal flora in the human oral cavity and pharynx. Furthermore, Rothia aeria, which was first isolated from air samples in the Russian space station Mir, is predicted to be an oral inhabitant. Immunocompromised patients are often infected by these organisms, leading to various systemic diseases. The involvement of these organisms in oral infections has attracted little attention, and their distribution in the oral cavity has not been fully clarified because of difficulties in accurately identifying these organisms. A suitable selective medium for oral Rothia species, including R. aeria, is necessary to assess the veritable prevalence of these organisms in the oral cavity. To examine the bacterial population in the oral cavity, a novel selective medium (ORSM) was developed for isolating oral Rothia species in this study. ORSM consists of tryptone, sodium gluconate, Lab-Lemco powder, sodium fluoride, neutral acriflavin, lincomycin, colistin, and agar. The average growth recovery of oral Rothia species on ORSM was 96.7% compared with that on BHI-Y agar. Growth of other representative oral bacteria, i.e. genera Streptococcus, Actinomyces, Neisseria, and Corynebacterium, was remarkably inhibited on the selective medium. PCR primers were designed based on partial sequences of the 16S rDNA genes of oral Rothia species. These primers reacted to each organism and did not react to other non-oral Rothia species or representative oral bacteria. These results indicated that these primers are useful for identifying oral Rothia species. A simple multiplex PCR procedure using these primers was a reliable method of identifying oral Rothia species. The proportion of oral Rothia species in saliva samples collected from 20 subjects was examined by culture method using ORSM. Rothia dentocariosa, Rothia mucilaginosa, and R. aeria accounted for 1.3%, 5.9%, and 0.8% of the total cultivable bacteria number on BHI-Y agar in the oral cavities of all subjects, respectively. It was indicated that among oral Rothia species, R. mucilaginosa is most predominant in the oral cavity of humans. A novel selective medium, ORSM, was useful for isolating each oral Rothia species.

Integrity signals

Registry

Crossref · 10.1016/j.mimet.2017.01.005

Cites

37 of 37 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Tsuzukibashi, O., Uchibori, S., Kobayashi, T., Umezawa, K., Mashimo, C., Nambu, T., Saito, M., Hashizume-Takizawa, T., & Ochiai, T.
Affiliations
  • Nihon University (JP)
  • Osaka Dental University (JP)
Corresponding author
Tsuzukibashi, Osamu
ORCID
Venue
Journal of Microbiological Methods
Publisher
Elsevier BV
Published
2017-03
Volume
134
Pages
21-26
ISSN
0167-7012 (ISSN-L)
DOI
10.1016/j.mimet.2017.01.005
OpenAlex
W2573528553
Cited by
74
References
36
Citations by year
2026: 5 · 2025: 12 · 2024: 7 · 2023: 10 · 2022: 9 · 2021: 13 · 2020: 7 · 2019: 8 · 2018: 3
FWCI
4.10
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Journal of Microbiological Methods (publishedVersion)
  • PubMed (publishedVersion)
Funders
Japan Society for the Promotion of Science (16K11469, 26463145, 16K11877)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Health Sciences › Medicine › Microbiology › Actinomycetales infections and treatment
Keywords
Rothia, Rothia dentocariosa, Rothia mucilaginosa, Rothia aeria, oral cavity, selective culture media, multiplex PCR, saliva, oral microbiota, Gram-positive bacteria, immunocompromised infections
MeSH terms
Agar, Culture Media, Gluconates, Humans, Micrococcaceae, Mouth, Peptones, Saliva, Polymerase Chain Reaction, DNA Primers

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review