Dorz1, a novel gene expressed in differentiating cerebellar granule neurons, is down-regulated in Zic1-deficient mouse
Abstract
The Zic1 gene encodes a zinc finger protein that controls vertebrate neural development. A previous study showed that Zic1 is expressed in developing and mature cerebellar granule neurons, and that Zic1-deficient cerebellum is hypoplastic and lacks a lobule of the anterior lobe. In the present study, we searched for genes de-regulated in the cerebellum to understand the molecular basis of cerebellar development. A novel gene, Dorz1, was identified and characterized as one of the most significantly down-regulated genes in Zic1-deficient cerebellum by the DNA microarray analysis. The expression of Dorz1 in the developing cerebellum peaked at embryonic day 17.5, and at that stage Dorz1 transcripts were detected in cerebellar granule neuron precursors where Zic1 expression is enhanced. In addition, Dorz1 expression was up-regulated in cultured cells overexpressing Zic1. These results suggest that Dorz1 expression is positively regulated by Zic1 during cerebellar development.
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We find a deleterious HHMC in ABHD14A, which is a target of ZIC1.
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From A catalog of single nucleotide changes distinguishing modern humans from archaic hominins (2019) · cites this paper as [88]
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- Authors
- Hoshino, J., Aruga, J., Ishiguro, A., & Mikoshiba, K.
- Affiliations
- RIKEN Center for Brain Science (JP)
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 2003-12
- Volume
- 120
- Issue
- 1
- Pages
- 57-64
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W2003674662
- Cited by
- 18
- References
- 21
- Citations by year
- 2026: 2 · 2025: 3 · 2023: 1 · 2019: 1 · 2018: 1 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Fetal and Pediatric Neurological Disorders
- Keywords
- ZIC1, cerebellar granule cells, cerebellar development, gene downregulation, granule neuron precursors, neural development, zinc finger proteins, gene expression regulation
- MeSH terms
- Aging, Amino Acid Sequence, Animals, Animals, Newborn, Brain, Cells, Cultured, Cerebellum, Cloning, Molecular, DNA-Binding Proteins, Embryo, Mammalian, Hydrolases, Immunohistochemistry, Indoles, Membrane Proteins, Mice, Neurologic Mutants, Neurons, Proteins, RNA, Messenger, Transcription Factors, Transfection, Blotting, Northern, Blotting, Western, Down-Regulation, Sequence Alignment, In Situ Hybridization, Gene Expression Regulation, Developmental, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, NIH 3T3 Cells, Mice
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