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Protease activation in glycerol-based deep eutectic solvents
Open accessAbstract
Deep eutectic solvents (DESs) consisting of mixtures of a choline salt (chloride or acetate form) and glycerol are prepared as easily accessible, biodegradable, and inexpensive alternatives to conventional aprotic cation-anion paired ionic liquids. These DES systems display excellent fluidity coupled with thermal stability to nearly 200 °C. In this work, the transesterification activities of cross-linked proteases (subtilisin and α-chymotrypsin), immobilized on chitosan, were individually examined in these novel DESs. In the 1:2 molar ratio mixture of choline chloride/glycerol containing 3% (v/v) water, cross-linked subtilisin exhibited an excellent activity (2.9 μmo l min−1 g−1) in conjunction with a selectivity of 98% in the transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol. These highly encouraging results advocate more extensive exploration of DESs in protease-mediated biotransformations of additional polar substrates and use of DESs in biocatalysis more generally.
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Cited by
Deep Eutectic Solvents (DESs) and Their Applications
Smith, E. L., Abbott, A. P., & Ryder, K. S. (2014) · Chemical Reviews · doi:10.1021/cr300162p · Cited by 7,378
Deep eutectic solvents: syntheses, properties and applications
Zhang, Q., De Oliveira Vigier, K., Royer, S., & Jérôme, F. (2012) · Chemical Society Reviews · doi:10.1039/c2cs35178a · Cited by 4,899
Deep eutectic solvents: A review of fundamentals and applications
Hansen, B. B., Spittle, S., Chen, B., Poe, D., Zhang, Y., Klein, J. M., Horton, A., Adhikari, L., Zelovich, T., Doherty, B. W., et al. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00385 · Cited by 3,422
Self-citationDeep Eutectic Solvents: Physicochemical Properties and Gas Separation Applications
García, G., Aparicio, S., Ullah, R., & Atilhan, M. (2015) · Energy & Fuels · doi:10.1021/ef5028873 · Cited by 1,112
Gu, Y., & Jérôme, F. (2013) · Chemical Society Reviews · doi:10.1039/c3cs60241a · Cited by 668
Metadata
- Authors
- Zhao, H., Baker, G. A., & Holmes, S.
- Venue
- Journal of Molecular Catalysis B: Enzymatic
- Publisher
- Elsevier BV
- Published
- 2011-11
- Volume
- 72
- Issue
- 3-4
- Pages
- 163-167
- ISSN
- 1381-1177 (ISSN-L)
- OpenAlex
- W2084637695
- Cited by
- 221
- References
- 3
- FWCI
- 4.93
- Type
- journal article
- Language
- en
- Open access
- green
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
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- Zhao, H., Baker, G. A., & Holmes, S. (2011). Protease activation in glycerol-based deep eutectic solvents. Journal of Molecular Catalysis B: Enzymatic. https://doi.org/10.1016/j.molcatb.2011.05.015
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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