Histone H2B deacetylation at lysine 11 is required for yeast apoptosis induced by phosphorylation of H2B at serine 10
Open accessAbstract
Chromatin alterations, induced by covalent histone modifications, mediate a wide range of DNA-templated processes, including apoptosis. Apoptotic chromatin condensation has been causally linked to the phosphorylation of histone H2B (serine 14 in human; serine 10 in yeast, H2BS10ph) in human and yeast cells. Here, we extend these studies by demonstrating a unidirectional, crosstalk pathway between H2BS10 phosphorylation and lysine 11 acetylation (H2BK11ac) in yeast. We demonstrate that the H2BK11 acetyl mark, which exists in growing yeast, is removed upon H(2)O(2) treatment but before H2BS10ph occurs, in a unidirectional fashion. H2B K11Q mutants are resistant to cell death elicited by H(2)O(2), while H2B K11R mutants that mimic deacetylation promote cell death. Our results suggest that Hos3 HDAC deacetylates H2BK11ac, which in turn mediates H2BS10ph by Ste20 kinase. Together, these studies underscore a concerted series of enzyme reactions governing histone modifications that promote a switch from cell proliferation to cell death.
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PCD can also occur during meiosis of diploid yeast cells, where it is regulated by histone H2B phosphorylation.
“PCD can also occur during meiosis of diploid yeast cells, where it is regulated by histone H2B phosphorylation (Ahn et al., 2006; Ahn et al., 2005).”
From Programmed cell death in aging (2015) · cites this paper as Ahn et al., 2006
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References 31 · Cited by 89
- Authors
- Ahn, S.-H., Diaz, R. L., Grunstein, M., & Allis, C. D.
- Affiliations
- Rockefeller University (US)
- University of California, Los Angeles (US)
- Corresponding author
- Allis, C. David
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2006-10
- Volume
- 24
- Issue
- 2
- Pages
- 211-220
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W1977088975
- Cited by
- 89
- References
- 31
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 7 · 2015: 5 · 2014: 3 · 2013: 8 · 2012: 4
- FWCI
- 3.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (GM-40922, R01GM040922, GM23674)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Histone Deacetylase Inhibitors Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- apoptosis, chromatin condensation, Ste20 kinase, histone modifications, hydrogen peroxide, Saccharomyces cerevisiae, cell death, histone acetylation
- MeSH terms
- Acetylation, Amino Acid Sequence, Chromatin, Histones, Lysine, Models, Biological, Molecular Sequence Data, Mutation, Phosphorylation, Saccharomyces cerevisiae, Serine, Apoptosis, Protein Serine-Threonine Kinases, MAP Kinase Kinase Kinases, Saccharomyces cerevisiae Proteins, Intracellular Signaling Peptides and Proteins, Cell Proliferation
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