The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Tracking Distinct RNA Populations Using Efficient and Reversible Covalent Chemistry
Open accessAbstract
SUMMARY We describe a chemical method to label and purify 4-thiouridine (s4U)-containing RNA. We demonstrate that methanethiosulfonate (MTS) reagents form disulfide bonds with s4U more efficiently than the commonly used HPDP-biotin, leading to higher yields and less biased enrichment. This increase in efficiency allowed us to use s4U-labeling to study global microRNA (miRNA) turnover in proliferating cultured human cells without perturbing global miRNA levels or the miRNA processing machinery. This improved chemistry will enhance methods that depend on tracking different populations of RNA, such as 4-thiouridine-tagging to study tissue-specific transcription and dynamic transcriptome analysis (DTA) to study RNA turnover.
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Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration
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miRBase: annotating high confidence microRNAs using deep sequencing data
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GENCODE: The reference human genome annotation for The ENCODE Project
Harrow, J., Frankish, A., Gonzalez, J. M., Tapanari, E., Diekhans, M., Kokocinski, F., Aken, B. L., Barrell, D., Zadissa, A., Searle, S., et al. (2012) · Genome Research · doi:10.1101/gr.135350.111 · Cited by 4,585
Self-citationModerated statistical tests for assessing differences in tag abundance
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Metadata
- Authors
- Duffy, E. E., Rutenberg-Schoenberg, M., Stark, C. D., Kitchen, R. R., Gerstein, M. B., & Simon, M. D.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2015-09
- Volume
- 59
- Issue
- 5
- Pages
- 858-866
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W1789593951
- Cited by
- 192
- References
- 5
- FWCI
- 9.26
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Duffy, E. E., Rutenberg-Schoenberg, M., Stark, C. D., Kitchen, R. R., Gerstein, M. B., & Simon, M. D. (2015). Tracking Distinct RNA Populations Using Efficient and Reversible Covalent Chemistry. Molecular Cell. https://doi.org/10.1016/j.molcel.2015.07.023
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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