The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
RNA Controls PolyQ Protein Phase Transitions
Open accessAbstract
Summary Compartmentalization in cells is central to the spatial and temporal control of biochemistry. In addition to membrane-bound organelles, membrane-less compartments form partitions in cells. Increasing evidence suggests that these compartments assemble through liquid-liquid phase separation. However the spatiotemporal control of their assembly, and how they maintain distinct functional and physical identities is poorly understood. We have previously shown an RNA-binding protein with a polyQ-expansion called Whi3 is essential for the spatial patterning of cyclin and formin transcripts in cytosol. Here, we show that specific mRNAs that are known physiological targets of Whi3 drive phase separation. mRNA can alter the viscosity of droplets, their propensity to fuse, and the exchange rates of components with bulk solution. Different mRNAs impart distinct biophysical properties of droplets indicating mRNA can bring individuality to assemblies. Our findings suggest that mRNAs can encode not only genetic information, but also the biophysical properties of phase-separated compartments.
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Cites
Genome Regulation by Long Noncoding RNAs
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A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation
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Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation
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Phase transitions in the assembly of multivalent signalling proteins
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Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins
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Highly inclined thin illumination enables clear single-molecule imaging in cells
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Cited by
Alberti, S., Gladfelter, A., & Mittag, T. (2019) · Cell · doi:10.1016/j.cell.2018.12.035 · Cited by 2,856
Self-citationThe nucleolus as a multiphase liquid condensate
Lafontaine, D. L. J., Riback, J. A., Bascetin, R., & Brangwynne, C. P. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0272-6 · Cited by 1,051
Self-citationSpatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets
Shin, Y., Berry, J., Pannucci, N., Haataja, M. P., Toettcher, J. E., & Brangwynne, C. P. (2017) · Cell · doi:10.1016/j.cell.2016.11.054 · Cited by 965
Self-citationα‐Synuclein aggregation nucleates through liquid‐liquid phase separation
Ray, S., Singh, N., Kumar, R., Patel, K., Pandey, S., Datta, D., Mahato, J., Panigrahi, R., Navalkar, A., Mehra, S., et al. (2020) · Nature Chemistry · doi:10.1038/s41557-020-0465-9 · Cited by 863
Distinct stages in stress granule assembly and disassembly
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Metadata
- Authors
- Zhang, H., Elbaum-Garfinkle, S., Langdon, E. M., Taylor, N., Occhipinti, P., Bridges, A. A., Brangwynne, C. P., & Gladfelter, A. S.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2015-10
- Volume
- 60
- Issue
- 2
- Pages
- 220-230
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2102898524
- Cited by
- 709
- References
- 38
- FWCI
- 21.36
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Zhang, H., Elbaum-Garfinkle, S., Langdon, E. M., Taylor, N., Occhipinti, P., Bridges, A. A., Brangwynne, C. P., & Gladfelter, A. S. (2015). RNA Controls PolyQ Protein Phase Transitions. Molecular Cell. https://doi.org/10.1016/j.molcel.2015.09.017
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