Regulation of Poly(A) Tail and Translation during the Somatic Cell Cycle
Open accessAbstract
Poly(A) tails are critical for mRNA stability and translation. However, recent studies have challenged this view, showing that poly(A) tail length and translation efficiency are decoupled in non-embryonic cells. Using TAIL-seq and ribosome profiling, we investigate poly(A) tail dynamics and translational control in the somatic cell cycle. We find dramatic changes in poly(A) tail lengths of cell-cycle regulatory genes like CDK1, TOP2A, and FBXO5, explaining their translational repression in M phase. We also find that poly(A) tail length is coupled to translation when the poly(A) tail is <20 nucleotides. However, as most genes have >20 nucleotide poly(A) tails, their translation is regulated mainly via poly(A) tail length-independent mechanisms during the cell cycle. Specifically, we find that terminal oligopyrimidine (TOP) tract-containing transcripts escape global translational suppression in M phase and are actively translated. Our quantitative and comprehensive data provide a revised view of translational control in the somatic cell cycle.
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The length of the poly(A) tail is proportional to translation efficiency.
“The poly(A) tail steadies mRNA and boosts protein translation, and the length of the poly(A) tail is proportional to translation efficiency.”
From Modifications of mRNA vaccine structural elements for improving mRNA stability and translation efficiency (2022) · cites this paper as Park et al. 2016
In “Translational Regulation and Poly(A) Tail Control during the Somatic Cell Cycle” (Figure 3B), the paper reports a relationship between tail length and translation efficiency only for short tails, up to about 20 nt. It says lengthening beyond that does not further influence translation, so the unqualified claim of proportionality overstates the finding. (LLM, conf=0.90)
Experimental — accuracy not yet measuredThe poly(A) tail is a critical factor in determining the longevity of mRNA molecules.
“It is a critical factor in determining the longevity of mRNA molecules (Park et al. 2016; Jalkanen et al. 2014).”
From Modifications of mRNA vaccine structural elements for improving mRNA stability and translation efficiency (2022) · cites this paper as Park et al. 2016
The poly(A) tail steadies mRNA and boosts protein translation.
“The poly(A) tail steadies mRNA and boosts protein translation, and the length of the poly(A) tail is proportional to translation efficiency.”
From Modifications of mRNA vaccine structural elements for improving mRNA stability and translation efficiency (2022) · cites this paper as Park et al. 2016
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Grant, G. D., Brooks, L., Zhang, X., Mahoney, J. M., Martyanov, V., Wood, T. A., Sherlock, G., Cheng, C., & Whitfield, M. L. (2013) · Molecular Biology of the Cell · doi:10.1091/mbc.e13-05-0264 · Cited by 179 · see citing works
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Research works printed but not linked: 5
- Baer, B.W., and Kornberg, R.D. (1980). Repeating structure of cytoplasmic poly(A)-ribonucleoprotein. Proc. Natl. Acad. Sci. USA 77, 1890-1892.
- Celis, J.E., Madsen, P., and Ryazanov, A.G. (1990). Increased phosphorylation of elongation factor 2 during mitosis in transformed human amnion cells corre- lates with a decreased rate of protein synthesis. Proc. Natl. Acad. Sci. USA 87, 4231-4235.
- Merbl, Y., Refour, P., Patel, H., Springer, M., and Kirschner, M.W. (2013). Profiling of ubiquitin-like modifications reveals features of mitotic control. Cell 152, 1160-1172.
- Shuda, M., Vela ´squez, C., Cheng, E., Cordek, D.G., Kwun, H.J., Chang, Y., and Moore, P.S. (2015). CDK1 substitutes for mTOR kinase to activate mitotic cap- dependent protein translation. Proc. Natl. Acad. Sci. USA 112, 5875-5882.
- Tanenbaum, M.E., Stern-Ginossar, N., Weissman, J.S., and Vale, R.D. (2015). Regulation of mRNA translation during mitosis. eLife 4, http://dx.doi.org/10. 7554/eLife.07957. · DOI
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Kim, S. C., Sekhon, S. S., Shin, W.-R., Ahn, G., Cho, B.-K., Ahn, J.-Y., & Kim, Y.-H. (2022) · Molecular & Cellular Toxicology · doi:10.1007/s13273-021-00171-4 · Cited by 255 · see citing works
Self-citationThe ribosome-engaged landscape of alternative splicing
Weatheritt, R. J., Sterne-Weiler, T., & Blencowe, B. J. (2016) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.3317 · Cited by 162 · see citing works
- Authors
- Park, J.-E., Yi, H., Kim, Y., Chang, H., & Kim, V. N.
- Affiliations
- Seoul National University (KR)
- Korea Advanced Institute of Science and Technology (KR)
- Corresponding author
- Kim, V. Narry
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 62
- Issue
- 3
- Pages
- 462-471
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2347006509
- Cited by
- 202
- References
- 1
- Citations by year
- 2026: 13 · 2025: 17 · 2024: 27 · 2023: 12 · 2022: 25 · 2021: 16 · 2020: 35 · 2019: 15 · 2018: 22 · 2017: 16 · 2016: 4
- FWCI
- 8.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- Seoul National University Open Repository (Seoul National University) (submittedVersion)
- Funders
- National Research Foundation
- National Research Foundation of Korea (IBS-R008-D1, NRF-2012)
- Institute for Basic Science (IBS-R008-D1, R008-D1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Keywords
- poly(A) tail length, mRNA stability, translational regulation, cell cycle, M phase, CDK1, TOP2A, ribosome profiling, TAIL-seq
- MeSH terms
- Poly-ADP-Ribose Binding Proteins, Antigens, Neoplasm, DNA Topoisomerases, Type II, DNA-Binding Proteins, Gene Expression Regulation, HeLa Cells, Humans, Mitosis, Oligodeoxyribonucleotides, Poly A, Poly dA-dT, Ribosomes, RNA, Messenger, Time Factors, Protein Biosynthesis, Gene Library, CDC2 Protein Kinase, Cell Cycle Proteins, Cyclin-Dependent Kinases, Gene Expression Profiling, RNA Stability, F-Box Proteins, M Phase Cell Cycle Checkpoints, S Phase Cell Cycle Checkpoints
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