The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
RNA-RNA Interactomes of ProQ and Hfq Reveal Overlapping and Competing Roles
Open accessAbstract
SUMMARY Base pairing RNAs modulate gene expression in all studied organisms. In many bacteria, the base pairing between most small regulatory RNAs (sRNAs) and their targets is mediated by the Hfq RNA chaperone. However, recent studies have shown FinO-domain proteins also bind sRNAs. To examine the global contribution of the FinO-domain ProQ protein in Escherichia coli, we carried out RIL-seq to identify RNA pairs bound to this protein. The RNA-RNA interactome for ProQ contains hundreds of pairs. Intriguingly, a significant fraction of the ProQ-bound RNA pairs also are found associated with Hfq, indicating overlapping, complementary or competing roles for the two proteins. Characterization of one novel RNA pair bound by both chaperones revealed that while Hfq is required for RNA sponge-mediated down regulation of the sRNA, ProQ can inhibit this regulation. Overall our results uncover increased complexity in RNA regulatory networks involving RNA chaperone proteins, RNases, sRNAs and mRNAs.
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Cites
Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
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Self-citationRetapamulin-Assisted Ribosome Profiling Reveals the Alternative Bacterial Proteome
Meydan, S., Marks, J., Klepacki, D., Sharma, V., Baranov, P. V., Firth, A. E., Margus, T., Kefi, A., Vázquez-Laslop, N., & Mankin, A. S. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.02.017 · Cited by 184
Cited by
Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation
Iosub, I. A., van Nues, R. W., McKellar, S. W., Nieken, K. J., Marchioretto, M., Sy, B., Tree, J. J., Viero, G., & Granneman, S. (2020) · eLife · doi:10.7554/elife.54655 · Cited by 98
Gerovac, M., El Mouali, Y., Kuper, J., Kisker, C., Barquist, L., & Vogel, J. (2020) · RNA · doi:10.1261/rna.076992.120 · Cited by 47
Gerovac, M., Wicke, L., Chihara, K., Schneider, C., Lavigne, R., & Vogel, J. (2021) · mBio · doi:10.1128/mbio.03454-20 · Cited by 40
Stein, E. M., Wang, S., Dailey, K. G., Gravel, C. M., Wang, S., Olejniczak, M., & Berry, K. E. (2023) · RNA · doi:10.1261/rna.079697.123 · Cited by 9
RAPDOR: Using Jensen-Shannon Distance for the computational analysis of complex proteomics datasets
Hemm, L., Rabsch, D., Ropp, H. R., Reimann, V., Gerth, P., Bartel, J., Brenes-Álvarez, M., Maaß, S., Becher, D., Hess, W. R., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-64086-7 · Cited by 5
Metadata
- Authors
- Melamed, S., Adams, P. P., Zhang, A., Zhang, H., & Storz, G.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2020-01
- Volume
- 77
- Issue
- 2
- Pages
- 411-425.e7
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2971833257
- Cited by
- 231
- References
- 3
- FWCI
- 15.17
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Melamed, S., Adams, P. P., Zhang, A., Zhang, H., & Storz, G. (2020). RNA-RNA Interactomes of ProQ and Hfq Reveal Overlapping and Competing Roles. Molecular Cell. https://doi.org/10.1016/j.molcel.2019.10.022
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