The DOI matches its Crossref record; 140 of the 140 references it cites were found in a registry or an index.
Structured elements drive extensive circular RNA translation
Open accessAbstract
SUMMARY The human genome encodes tens of thousands circular RNAs (circRNAs) with mostly unknown functions. Circular RNAs requires internal ribosome entry sites (IRES) if they are to undergo translation without 5’ cap. Here, we develop a high-throughput screen to systematically discover RNA sequences that can direct circRNA translation in human cells. We identify over 17,000 endogenous and synthetic sequences as candidate circRNA IRES. 18S rRNA complementarity and a structured RNA element positioned on the IRES are important for driving circRNA translation. Ribosome profiling and peptidomic analyses show extensive IRES-ribosome association, hundreds of circRNA-encoded proteins with tissue -specific distribution, and antigen presentation. We find that circFGFR1p, a protein encoded by circFGFR1 that is down regulated in cancer, functions as a negative regulator of FGFR1 oncoprotein to suppress cell growth during stress. Systematic identification of circRNA IRES elements may provide important links among circRNA regulation, biological function, and disease.
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Circular RNAs are abundant, conserved, and associated with ALU repeats
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Self-citationcircRNA biogenesis competes with pre-mRNA splicing
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Engineering circular RNA for enhanced protein production
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Self-citationBiogenesis and Regulatory Roles of Circular RNAs
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Circular RNA vaccine in disease prevention and treatment
Niu, D., Wu, Y., & Lian, J. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01561-x · Cited by 267
Conn, V. M., Chinnaiyan, A. M., & Conn, S. J. (2024) · Nature Reviews Cancer · doi:10.1038/s41568-024-00721-7 · Cited by 258
Metadata
- Authors
- Chen, C.-K., Cheng, R., Demeter, J., Chen, J., Weingarten-Gabbay, S., Jiang, L., Snyder, M. P., Weissman, J. S., Segal, E., Jackson, P. K., & Chang, H. Y.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2021-10
- Volume
- 81
- Issue
- 20
- Pages
- 4300-4318.e13
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W3195559098
- Cited by
- 316
- References
- 140
- FWCI
- 13.28
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Chen, C.-K., Cheng, R., Demeter, J., Chen, J., Weingarten-Gabbay, S., Jiang, L., Snyder, M. P., Weissman, J. S., Segal, E., Jackson, P. K., & Chang, H. Y. (2021). Structured elements drive extensive circular RNA translation. Molecular Cell. https://doi.org/10.1016/j.molcel.2021.07.042
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