The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
Engineering circular RNA for enhanced protein production
Open accessAbstract
Circular RNAs (circRNAs) are stable and prevalent RNAs in eukaryotic cells that arise from back-splicing. Synthetic circRNAs and some endogenous circRNAs can encode proteins, raising the promise of circRNA as a platform for gene expression. In this study, we developed a systematic approach for rapid assembly and testing of features that affect protein production from synthetic circRNAs. To maximize circRNA translation, we optimized five elements: vector topology, 5′ and 3′ untranslated regions, internal ribosome entry sites and synthetic aptamers recruiting translation initiation machinery. Together, these design principles improve circRNA protein yields by several hundred-fold, provide increased translation over messenger RNA in vitro, provide more durable translation in vivo and are generalizable across multiple transgenes.
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Metadata
- Authors
- Chen, R., Wang, S. K., Belk, J. A., Amaya, L., Li, Z., Cardenas, A., Abe, B. T., Chen, C.-K., Wender, P. A., & Chang, H. Y.
- Venue
- Nature Biotechnology
- Published
- 2022-07-18
- Volume
- 41
- Issue
- 2
- Pages
- 262-272
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W4285728587
- Cited by
- 455
- References
- 53
- FWCI
- 22.86
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Chen, R., Wang, S. K., Belk, J. A., Amaya, L., Li, Z., Cardenas, A., Abe, B. T., Chen, C.-K., Wender, P. A., & Chang, H. Y. (2023). Engineering circular RNA for enhanced protein production. Nature Biotechnology. https://doi.org/10.1038/s41587-022-01393-0
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