Comprehensive analysis of microRNA expression profile in malignant glioma tissues
Open accessAbstract
Malignant gliomas represent the most devastating group of brain tumors in adults, among which glioblastoma multiforme (GBM) exhibits the highest malignancy rate. Despite combined modality treatment, GBM recurs and is invariably fatal. A further insight into the molecular background of gliomagenesis is required to improve patient outcomes. The primary aim of this study was to gain broad information on the miRNA expression pattern in malignant gliomas, mainly GBM. We investigated the global miRNA profile of malignant glioma tissues with miRNA microarrays, deep sequencing and meta‐analysis. We selected miRNAs that were most frequently deregulated in glioblastoma tissues, as well as in peritumoral areas, in comparison with normal human brain. We identified candidate miRNAs associated with the progression from glioma grade III to glioma grade IV. The meta‐analysis of miRNA profiling studies in GBM tissues summarizes the past and recent advances in the investigation of the miRNA signature in GBM versus noncancerous human brain and provides a comprehensive overview. We propose a list of 35 miRNAs whose expression is most frequently deregulated in GBM patients and of 30 miRNA candidates recognized as novel GBM biomarkers.
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No miR-21 expression was detected in adjacent non-malignant brain tissue, indicating its elevated expression was localized to tumor cells.
“No miR-21 expression was detected in adjacent non-malignant brain tissue, indicating its elevated expression was localized to tumor cells [28]”
From miR-21 as a diagnostic and prognostic biomarker in glioma: tissue and serum expression analysis in a Pakistani cohort (2026) · cites this paper as [28]
Section 3.1 identifies miR-21 as up-regulated in both tumor and adjacent peritumoral tissue, contrary to the claim that it was undetected in adjacent tissue. Section 4.1 likewise reports miR-21 overexpression among changes in adjacent brain, so the paper does not support localization of its elevated expression to tumor tissue. (LLM, conf=0.91)
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Experimental — accuracy not yet measuredmiR-21 expression level significantly increases with tumor grade.
“miR-21 expression level significantly increased with tumor grade, consistent with previous studies [27], [28].”
From miR-21 as a diagnostic and prognostic biomarker in glioma: tissue and serum expression analysis in a Pakistani cohort (2026) · cites this paper as [28]
Section 4.3 (Meta-analysis; page unavailable) reports that miR-21 is frequently up-regulated in glioblastoma versus noncancerous brain and describes it as up-regulated in high-grade gliomas. Those comparisons do not establish that miR-21 expression increases significantly as tumor grade increases; the grade III–IV comparison in section 3.2 does not identify miR-21 among the differentially expressed miRNAs. (LLM, conf=0.83)
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Cites
Most mammalian mRNAs are conserved targets of microRNAs
Friedman, R. C., Farh, K. K.-H., Burge, C. B., & Bartel, D. P. (2009) · Genome Research · doi:10.1101/gr.082701.108 · Cited by 8,462 · see citing works
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The Somatic Genomic Landscape of Glioblastoma
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Skalsky, R. L., & Cullen, B. R. (2011) · PLoS ONE · doi:10.1371/journal.pone.0024248 · Cited by 170 · see citing works
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Cited by
Sorted by citations
Inhibition of miR-21 in glioma cells using catalytic nucleic acids
Belter, A., Rolle, K., Piwecka, M., Fedoruk-Wyszomirska, A., Naskręt-Barciszewska, M. Z., & Barciszewski, J. (2016) · Scientific Reports · doi:10.1038/srep24516 · Cited by 36 · see citing works
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“Low miR-21 expression was detected in adjacent non-malignant brain tissue, indicating its elevated expression was localized to tumor cells [28].”
- Authors
- Piwecka, M., Rolle, K., Belter, A., Barciszewska, A. M., Żywicki, M., Michalak, M., Nowak, S., Naskręt-Barciszewska, M. Z., & Barciszewski, J.
- Affiliations
- Institute of Bioorganic Chemistry, Polish Academy of Sciences (PL)
- Poznan University of Medical Sciences (PL)
- Adam Mickiewicz University in Poznań (PL)
- Institute of Dendrology (PL)
- Corresponding author
- Barciszewski, Jan
- ORCID
- Venue
- Molecular Oncology
- Publisher
- Wiley
- Published
- 2015-03-25
- Volume
- 9
- Issue
- 7
- Pages
- 1324-1340
- ISSN
- 1574-7891 (ISSN-L)
- 1878-0261
- PMC
- PMC5528820
- OpenAlex
- W2095801710
- Cited by
- 108
- References
- 5
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 9 · 2023: 8 · 2022: 14 · 2021: 28 · 2020: 9 · 2019: 12 · 2018: 5 · 2017: 6 · 2016: 9 · 2015: 4
- FWCI
- 2.64
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Oncology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- European Regional Development Fund (01.03.01-30-050/09)
- National Science Centre (5955/B/P01/2010/38 (KR))
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- miRNA expression profiling, high-grade glioma, glioblastoma, microRNA dysregulation, microRNA biomarkers, gliomagenesis
- MeSH terms
- Glioblastoma, Humans, Biomarkers, Gene Expression Profiling, MicroRNAs, High-Throughput Nucleotide Sequencing
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