Cited by 36MicroRNA in disease regulationRNA Interference and Gene DeliveryCancer-related molecular mechanisms research

Inhibition of miR-21 in glioma cells using catalytic nucleic acids

Open access
Belter, Agnieszka, Rolle, Katarzyna, Piwecka, Monika, Fedoruk-Wyszomirska, Agnieszka, Naskręt-Barciszewska, Mirosława Z., Barciszewski, Jan·2016·Scientific Reports

Abstract

Despite tremendous efforts worldwide, glioblastoma multiforme (GBM) remains a deadly disease for which no cure is available and prognosis is very bad. Recently, miR-21 has emerged as a key omnipotent player in carcinogenesis, including brain tumors. It is recognized as an indicator of glioma prognosis and a prosperous target for anti-tumor therapy. Here we show that rationally designed hammerhead ribozymes and DNAzymes can target miR-21 and/or its precursors. They decrease miR-21 level and thus silence this oncomiR functions. We demonstrated that anti-miRNA catalytic nucleic acids show a novel terrific arsenal for specific and effective combat against diseases with elevated cellular miR-21 content, such as brain tumors.

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Research works printed but not linked: 4

  1. Uhlenbeck O. C. A small catalytic oligonucleotide. Nature 328, 596– 600 (1987). 2441261
  2. Santoro S. W. & Joyce G. F. Mechanism and utility of an RNA-cleavage enzyme. Biochemistry 37, 13330– 13342 (1998). 9748341
  3. Zhou X.. Downregulation of miR-21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status. Lab. Invest. 90, 144– 155 (2010). 20048743
  4. Wang T. T.. MiR-21 modulates hTERT through a STAT3-dependent manner on glioblastoma cell growth. CNS Neurosci. Ther. 18, 722– 728 (2012). 22709411 PMC6493453

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Authors
Belter, A., Rolle, K., Piwecka, M., Fedoruk-Wyszomirska, A., Naskręt-Barciszewska, M. Z., & Barciszewski, J.
Affiliations
Institute of Bioorganic Chemistry, Polish Academy of Sciences (PL)
Corresponding author
Barciszewski, Jan
Venue
Scientific Reports
Published
2016-04-15
Volume
6
Issue
1
Pages
24516-24516
ISSN
2045-2322 (ISSN-L)
OpenAlex
W2338940124
Cited by
36
References
89
Citations by year
2026: 3 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 2 · 2019: 5 · 2018: 3 · 2017: 6 · 2016: 1
FWCI
2.08
Type
journal article
Language
en
Open access
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
Keywords
miR-21, glioma, glioblastoma, hammerhead ribozyme, DNAzyme, catalytic nucleic acids, anti-miRNA, microRNA inhibition, oncogenic microRNA, brain tumors, anti-tumor therapy
MeSH terms
Brain Neoplasms, Glioma, Humans, Hydrolysis, Gene Expression Regulation, Neoplastic, RNA, Catalytic, DNA, Catalytic, MicroRNAs, Cell Line, Tumor

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