Inhibition of miR-21 in glioma cells using catalytic nucleic acids
Open accessAbstract
Despite tremendous efforts worldwide, glioblastoma multiforme (GBM) remains a deadly disease for which no cure is available and prognosis is very bad. Recently, miR-21 has emerged as a key omnipotent player in carcinogenesis, including brain tumors. It is recognized as an indicator of glioma prognosis and a prosperous target for anti-tumor therapy. Here we show that rationally designed hammerhead ribozymes and DNAzymes can target miR-21 and/or its precursors. They decrease miR-21 level and thus silence this oncomiR functions. We demonstrated that anti-miRNA catalytic nucleic acids show a novel terrific arsenal for specific and effective combat against diseases with elevated cellular miR-21 content, such as brain tumors.
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Inhibition of miR-21 via different strategies elevated apoptotic cell death, sensitivity to chemotherapy/radiotherapy, and diminished tumor progression.
“Inhibition of miR-21 via different strategies elevated apoptotic cell death, sensitivity to chemotherapy/radiotherapy, and diminished tumor progression (24–28).”
From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [26]
miR-21 silencing has demonstrated effective results both in vitro and in vivo.
“In addition, miR-21 silencing has demonstrated effective results both in vitro and in vivo (24–28).”
From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [26]
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- Authors
- Belter, A., Rolle, K., Piwecka, M., Fedoruk-Wyszomirska, A., Naskręt-Barciszewska, M. Z., & Barciszewski, J.
- Affiliations
- Institute of Bioorganic Chemistry, Polish Academy of Sciences (PL)
- Corresponding author
- Barciszewski, Jan
- ORCID
- Venue
- Scientific Reports
- Published
- 2016-04-15
- Volume
- 6
- Issue
- 1
- Pages
- 24516-24516
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4832220
- OpenAlex
- W2338940124
- Cited by
- 36
- References
- 89
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 2 · 2019: 5 · 2018: 3 · 2017: 6 · 2016: 1
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- miR-21, glioma, glioblastoma, hammerhead ribozyme, DNAzyme, catalytic nucleic acids, anti-miRNA, microRNA inhibition, oncogenic microRNA, brain tumors, anti-tumor therapy
- MeSH terms
- Brain Neoplasms, Glioma, Humans, Hydrolysis, Gene Expression Regulation, Neoplastic, RNA, Catalytic, DNA, Catalytic, MicroRNAs, Cell Line, Tumor
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