Comet assay and analysis of micronucleus formation in patients with rheumatoid arthritis.
Abstract
Oxidants play a significant role in causing oxidative stress, which underlies the pathogenesis of rheumatoid arthritis (RA). Genetic factors that predispose individuals to RA are considered to play an important role in the development of the disease. The aim of this study was to determine, by use of the comet assay and the micronucleus (MN) test, whether DNA damage has an effect on the pathogenesis of RA. Furthermore, our aim was to show if there is an association between oxidative stress and DNA damage in RA. This study was conducted between January and June 2010 in the Erzurum Training and Research Hospital. We analyzed lymphocytes from patients with RA (12 in active and 31 in inactive periods) and 30 healthy controls for effects in the comet assay and the MN test. In addition, the levels of malondialdehyde (MDA) and superoxide dismutase (SOD), the activity of glutathione peroxidase (GSH-Px), the erythrocyte sedimentation rate (ESR) and the high-sensitivity C-reactive protein (hs-CRP) rate were determined in all the subjects. The comet-tail length, the MN frequencies and the MDA levels were significantly higher in patients--both in the active and the inactive period--than in the controls. In contrast, the SOD and GSH-Px levels were significantly lower in both patient groups than in the controls. Our results suggest that an increased plasma MDA level and decreased plasma GSH-Px and SOD levels reflect the higher degree of oxidative stress in RA patients, a situation that may impair genetic stability in those patients. Thus, the results suggest that increased DNA damage may play an important role in the pathogenesis of RA.
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Comet assay tail lengths are elevated in rheumatoid arthritis cells from patients with active and inactive disease, indicating a complex relationship between inflammation and the DNA repair defect.
“Elevated tail length measurements in comet assays of RA cells have been reported in patients with active and inactive disease, suggesting a complicated relationship exists between the inflammatory milieu and the DNA repair defect [75].”
From T-cell aging in rheumatoid arthritis (2014) · cites this paper as [75]
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T-cell aging in rheumatoid arthritis
Weyand, C. M., Yang, Z., & Goronzy, J. J. (2014) · Current Opinion in Rheumatology · doi:10.1097/bor.0000000000000011 · Cited by 164 · see citing works
- Authors
- Karaman, A., Binici, D. N., & Melikoğlu, M. A.
- Affiliations
- Erzurum Regional Training and Research Hospital (TR)
- Corresponding author
- Karaman, Ali
- Venue
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- Publisher
- Elsevier BV
- Published
- 2011-03
- Volume
- 721
- Issue
- 1
- Pages
- 1-5
- ISSN
- 1383-5718 (ISSN-L)
- OpenAlex
- W1976956247
- Cited by
- 39
- References
- 59
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 7 · 2023: 3 · 2022: 5 · 2021: 1 · 2020: 4 · 2018: 4 · 2016: 5 · 2015: 2 · 2013: 2 · 2012: 3
- FWCI
- 1.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- rheumatoid arthritis, oxidative stress, DNA damage, micronucleus formation, malondialdehyde, superoxide dismutase, glutathione peroxidase, lymphocytes, genetic stability, disease activity, C-reactive protein, erythrocyte sedimentation rate
- MeSH terms
- Arthritis, Rheumatoid, Blood Sedimentation, C-Reactive Protein, DNA Damage, Female, Glutathione Peroxidase, Humans, Male, Malondialdehyde, Superoxide Dismutase, Micronucleus Tests, Oxidative Stress, Comet Assay
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