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Cited by 16Radiation Therapy and DosimetryEffects of Radiation ExposureDNA Repair Mechanisms

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Sub-micrometer 20 MeV protons or 45 MeV lithium spot irradiation enhances yields of dicentric chromosomes due to clustering of DNA double-strand breaks

Schmid, T.E., Friedland, W., Greubel, C., Girst, S., Reindl, J., Siebenwirth, C., Ilicic, K., Schmid, E., Multhoff, G., Schmitt, E., Kundrát, P., Dollinger, G.·2015·Mutation Research/Genetic Toxicology and Environmental Mutagenesis
DOI 10.1016/j.mrgentox.2015.07.015
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Abstract

In conventional experiments on biological effects of radiation types of diverse quality, micrometer-scale double-strand break (DSB) clustering is inherently interlinked with clustering of energy deposition events on nanometer scale relevant for DSB induction. Due to this limitation, the role of the micrometer and nanometer scales in diverse biological endpoints cannot be fully separated. To address this issue, hybrid human-hamster AL cells have been irradiated with 45MeV (60keV/μm) lithium ions or 20MeV (2.6keV/μm) protons quasi-homogeneously distributed or focused to 0.5×1μm(2) spots on regular matrix patterns (point distances up to 10.6×10.6μm), with pre-defined particle numbers per spot to provide the same mean dose of 1.7Gy. The yields of dicentrics and their distribution among cells have been scored. In parallel, track-structure based simulations of DSB induction and chromosome aberration formation with PARTRAC have been performed. The results show that the sub-micrometer beam focusing does not enhance DSB yields, but significantly affects the DSB distribution within the nucleus and increases the chance to form DSB pairs in close proximity, which may lead to increased yields of chromosome aberrations. Indeed, the experiments show that focusing 20 lithium ions or 451 protons per spot on a 10.6μm grid induces two or three times more dicentrics, respectively, than a quasi-homogenous irradiation. The simulations reproduce the data in part, but in part suggest more complex be…

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Research works printed but not linked: 27

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Metadata

Authors
Schmid, T. E., Friedland, W., Greubel, C., Girst, S., Reindl, J., Siebenwirth, C., Ilicic, K., Schmid, E., Multhoff, G., Schmitt, E., Kundrát, P., & Dollinger, G.
Affiliations
  • Technical University of Munich (DE)
  • Helmholtz Munich (DE)
  • Universität der Bundeswehr München (DE)
  • Ludwig-Maximilians-Universität München (DE)
Corresponding author
Schmid, T.E.
ORCID
Venue
Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Publisher
Elsevier BV
Published
2015-11
Volume
793
Pages
30-40
ISSN
1383-5718 (ISSN-L)
DOI
10.1016/j.mrgentox.2015.07.015
OpenAlex
W1922813002
Cited by
16
References
0
Citations by year
2024: 3 · 2023: 1 · 2021: 1 · 2019: 1 · 2018: 6 · 2017: 3 · 2016: 1
FWCI
2.64
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Mutation Research/Genetic Toxicology and Environmental Mutagenesis (publishedVersion)
  • PubMed (publishedVersion)
  • mediaTUM (Technical University of Munich) (submittedVersion)
  • Site cant be reached (submittedVersion)
  • PuSH - Publication Server of Helmholtz Zentrum München (submittedVersion)
Topics
  • Physical Sciences › Physics and Astronomy › Radiation › Radiation Therapy and Dosimetry
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords
dicentric chromosomes, lithium ions, protons, high-LET radiation, chromosomal abnormalities, track structure simulation, energy deposition, overkill, saturation, biophysical model
MeSH terms
Animals, Chromosome Aberrations, Chromosomes, Human, Pair 11, Cricetulus, Humans, Lithium, Models, Genetic, Models, Theoretical, Protons, Relative Biological Effectiveness, CHO Cells, Chromosomes, Mammalian, DNA Breaks, Double-Stranded

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