Gelatin functionalised porous titanium alloy implants for orthopaedic applications
Abstract
In the present work, we studied the immobilisation of the biopolymer gelatin onto the surface of three dimensional (3D) regular Ti6Al4V porous implants to improve their surface bio-activity. The successful immobilisation of the gelatin coating was made possible by a polydopamine interlayer, a polymer coating inspired by the adhesive nature of mussels. The presence of both coatings was first optimised on two dimensional titanium (2D Ti) substrates and confirmed by different techniques including X-ray photelectron spectroscopy, contact angle measurements, atomic force microscopy and fluorescence microscopy. Results showed homogeneous coatings that are stable for at least 24h in phosphate buffer at 37°C. In a next step, the coating procedure was successfully transferred to 3D Ti6Al4V porous implants, which indicates the versatility of the applied coating procedure with regard to complex surface morphologies. Furthermore, the bio-activity of these stable gelatin coatings was enhanced by applying a third and final coating using the cell-attractive protein fibronectin. The reproducible immobilisation process allowed for a controlled biomolecule presentation to the surrounding tissue. This newly developed coating procedure outperformed the previously reported silanisation procedure for immobilising gelatin. In vitro cell adhesion and culture studies with human periosteum-derived cells showed that the investigated coatings did not compromise the biocompatible nature of Ti6Al4V porous…
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For a polydopamine-coated surface of poly-L-lactic acid (PLLA) biosubstitute, the polydopamine coating layer enhances the immunogenicity and biocompatibility of the biosubstitute material.
“For a PDA-coated surface of poly-L-lactic acid (PLLA) biosubstitute, the PDA coating layer reduced the blood toxicity of CdSe quantum dots while it enhanced the immunogenicity and the biocompatibility of biosubstitute material [80].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [80]
For a polydopamine-coated surface of poly-L-lactic acid (PLLA) biosubstitute, the polydopamine coating layer reduces the blood toxicity of CdSe quantum dots.
“For a PDA-coated surface of poly-L-lactic acid (PLLA) biosubstitute, the PDA coating layer reduced the blood toxicity of CdSe quantum dots while it enhanced the immunogenicity and the biocompatibility of biosubstitute material [80].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [80]
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Polydopamine-Assisted Surface Modification for Bone Biosubstitutes
Huang, S., Liang, N., Hu, Y., Zhou, X., & Abidi, N. (2016) · BioMed Research International · doi:10.1155/2016/2389895 · Cited by 97 · see citing works
“Compared to traditional immobilization approaches, PDA-assisted immobilization exhibits a merit to significantly improve the anchoring performance of biomolecules [79].”
Background
- Authors
- Vanderleyden, E., Van Bael, S., Chai, Y. C., Kruth, J.-P., Schrooten, J., & Dubruel, P.
- Affiliations
- Ghent University (BE)
- KU Leuven (BE)
- Corresponding author
- Dubruel, P.
- Venue
- Materials Science and Engineering: C
- Publisher
- Elsevier BV
- Published
- 2014-09
- Volume
- 42
- Pages
- 396-404
- ISSN
- 0928-4931 (ISSN-L)
- OpenAlex
- W2067848709
- Cited by
- 45
- References
- 0
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 2 · 2023: 4 · 2022: 6 · 2021: 5 · 2020: 3 · 2019: 5 · 2018: 4 · 2017: 3 · 2016: 3 · 2015: 4
- FWCI
- 1.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Materials Science and Engineering C (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Lirias (submittedVersion)
- Funders
- Fonds Wetenschappelijk Onderzoek
- KU Leuven
- Universiteit Gent
- Vlaamse regering
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Materials Chemistry › Titanium Alloys Microstructure and Properties
- Physical Sciences › Engineering › Mechanical Engineering › Surface Treatment and Coatings
- Keywords
- porous titanium implants, Ti-6Al-4V, polydopamine coating, fibronectin coating, surface bioactivity, orthopedic implants, periosteum-derived cells, cell adhesion
- MeSH terms
- Adolescent, Alloys, Cell Differentiation, Cell Survival, Cells, Cultured, Child, Gelatin, Humans, Indoles, Materials Testing, Periosteum, Polymers, Surface Properties, Titanium, Porosity, Orthopedic Procedures, Prostheses and Implants, Coated Materials, Biocompatible
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