In vitro evaluation of folate-modified PLGA nanoparticles containing paclitaxel for ovarian cancer therapy
Abstract
Ovarian cancer is the most lethal gynecological cancer of female reproductive system. In order to improve the survival rate, some modifications on nanoparticles surfaces have been investigated to promote active targeting of drugs into tumor microenvironment. The aim of this study was the development and characterization of folate-modified (PN-PCX-FA) and unmodified PLGA nanoparticles (PN-PCX) containing paclitaxel for ovarian cancer treatment. Nanocarriers were produced using nanoprecipitation technique and characterized by mean particle diameter (MPD), polydispersity index (PDI), zeta potential (ZP), encapsulation efficiency (EE), DSC, FTIR, in vitro cytotoxicity and cellular uptake. PN-PCX and PN-PCX-FA showed MPD < 150 nm and PDI < 0.2 with high EE (about 90%). Cytotoxicity assays in SKOV-3 cells demonstrated the ability of both formulations to cause cellular damage. PCX encapsulated in PN-PCX-FA at 1 nM showed higher cytotoxicity than PN-PCX. Folate-modified nanoparticles showed a 3.6-fold higher cellular uptake than unmodified nanoparticles. PN-PCX-FA is a promising system to improve safety and efficacy of ovarian cancer treatment. Further in vivo studies are necessary to prove PN-PCX-FA potential.
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Folate receptor is overexpressed on the surface of a variety of cancers including breast, kidney, colorectal, brain, and ovarian cancers.
“The folate receptor (FR) is overexpressed on the surface of a variety of cancers, including breast, kidney, colorectal, brain, and ovarian cancers.263–265”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [263]
Folic acid-modified nanoparticles show 3.6 times higher cellular uptake than unmodified nanoparticles.
“The folic acid-modified nanoparticles constructed by Luiz et al. also showed 3.6 times higher cellular uptake than unmodified nanoparticles.263”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [263]
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References 47 · Cited by 63
- Authors
- Luiz, M. T., Abriata, J. P., Raspantini, G. L., Tofani, L. B., Fumagalli, F., de Melo, S. M. G., Emery, F. D. S., Swiech, K., Marcato, P. D., Lee, R., & Marchetti, J. M.
- Affiliations
- Universidade de São Paulo (BR)
- The Ohio State University (US)
- Corresponding author
- Marchetti, Juliana Maldonado
- ORCID
- Venue
- Materials Science and Engineering: C
- Publisher
- Elsevier BV
- Published
- 2019-12
- Volume
- 105
- Pages
- 110038
- ISSN
- 0928-4931 (ISSN-L)
- OpenAlex
- W2966871100
- Cited by
- 63
- References
- 47
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 8 · 2023: 10 · 2022: 9 · 2021: 19 · 2020: 6 · 2019: 1
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Materials Science and Engineering C (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (132910/2016-0)
- Fundação de Amparo a Pesquisa do Estado de São Paulo (2016/03170-2)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Health Sciences › Medicine › Reproductive Medicine › Ovarian cancer diagnosis and treatment
- Keywords
- ovarian cancer, paclitaxel, PLGA nanoparticles, targeted drug delivery, nanoprecipitation, cellular uptake, cytotoxicity, SKOV3 cells, nanocarriers, encapsulation efficiency
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Boron Compounds, Calorimetry, Differential Scanning, Endocytosis, Female, Folic Acid, Humans, Ovarian Neoplasms, Particle Size, Cell Death, Paclitaxel, Spectroscopy, Fourier Transform Infrared, Cell Line, Tumor, Nanoparticles
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